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Active collagen lyophilized powder and preparation method thereof

A collagen and freeze-dried powder technology, applied in the field of collagen, can solve the problems of reduced collagen stability, reduced collagen efficacy, no collagen biological activity, etc., achieves good application prospects, high dissolved concentration, and promotes skin collagen. synthetic effect

Active Publication Date: 2020-09-18
GUANGDONG ZHONGERJIAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing lyophilized collagen still has the following deficiencies: 1) the special amino acid content and ratio of collagen make it difficult to redissolve the lyophilized collagen. Generally, the dissolved concentration of lyophilized collagen is not higher than 0.2%; 2) is To increase the dissolving ability of natural active collagen, some groups in natural collagen are usually modified by means of acylation and esterification, which reduces the stability of collagen itself and makes the arginine in active collagen -Arginine in glycine-aspartic acid changes the structure, reduces the activity of collagen to promote cell growth, and further leads to the reduction or loss of collagen efficacy; Collagen peptide, does not have the biological activity of collagen

Method used

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  • Active collagen lyophilized powder and preparation method thereof
  • Active collagen lyophilized powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation method of the active collagen freeze-dried powder of the present embodiment comprises the following steps:

[0039] 1) The collagen in the tail tendon of SD rats of SPF grade is extracted by acid-enzyme mixing method;

[0040] 2) Take 3 parts of 100mL collagen extracted in step 1), freeze-dry, and dry at 37°C for 24 hours, weigh the weight m (g) of the collagen after freeze-drying, and calculate the average value That is, the concentration of this batch of collagen;

[0041] 3) Adjust the concentration of the collagen obtained in step 2) to 4g / L with sterilized purified water;

[0042] 4) Take 100mL of 4g / L collagen, add 4g of mannitol, stir and mix until completely dissolved;

[0043] 5) Add 2 g of trehalose to the mixed solution obtained in step 4), stir and mix until completely dissolved;

[0044] 6) Add 0.1 g of citric acid to the mixed solution obtained in step 5), stir and mix until completely dissolved;

[0045] 7) The mixed solution obtained...

Embodiment 2

[0049]The preparation method of the active collagen freeze-dried powder of the present embodiment comprises the following steps:

[0050] 1) The collagen in the tail tendon of SD rats of SPF grade is extracted by acid-enzyme mixing method;

[0051] 2) Take 3 parts of 100mL collagen extracted in step 1), freeze-dry, and dry at 37°C for 24 hours, weigh the weight m (g) of the collagen after freeze-drying, and calculate the average value That is, the concentration of this batch of collagen;

[0052] 3) Adjust the concentration of the collagen obtained in step 2) to 4g / L with sterilized purified water;

[0053] 4) Take 100mL of 4g / L collagen, add 6g of mannitol, stir and mix until completely dissolved;

[0054] 5) Add 2 g of trehalose to the mixed solution obtained in step 4), stir and mix until completely dissolved;

[0055] 6) Add 0.1 g of citric acid to the mixed solution obtained in step 5), stir and mix until completely dissolved;

[0056] 7) The mixed solution obtained ...

Embodiment 3

[0058] The preparation method of the active collagen freeze-dried powder of the present embodiment comprises the following steps:

[0059] 1) The collagen in the tail tendon of SD rats of SPF grade is extracted by acid-enzyme mixing method;

[0060] 2) Take 3 parts of 100mL collagen extracted in step 1), freeze-dry, and dry at 37°C for 24 hours, weigh the weight m (g) of the collagen after freeze-drying, and calculate the average value That is, the concentration of this batch of collagen;

[0061] 3) Adjust the concentration of the collagen obtained in step 2) to 4g / L with sterilized purified water;

[0062] 4) Take 100mL of 4g / L collagen, add 10g of mannitol, stir and mix until completely dissolved;

[0063] 5) Add 2 g of trehalose to the mixed solution obtained in step 4), stir and mix until completely dissolved;

[0064] 6) Add 0.1 g of citric acid to the mixed solution obtained in step 5), stir and mix until completely dissolved;

[0065] 7) The mixed solution obtaine...

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Abstract

The present invention discloses an active collagen lyophilized powder and a preparation method thereof. A rat tail collagen, polyols, mycose and citric acid are compounded, so that the stability of the rat tail collagen in a freeze-drying process may be jointly improved, a prepared lyophilized product may be stored without a preservative for a long time and is good in redissolution property, capable of keeping the natural bioactivity of collagen and remarkable in capability of promoting the synthesis of collagen in the skin.

Description

technical field [0001] The invention relates to the technical field of collagen, in particular to a freeze-dried active collagen powder and a preparation method thereof. Background technique [0002] Collagen is widely found in various vertebrates and is a structural protein that provides mechanical strength to connective tissues such as tendons, bones, cartilage, teeth, skin and blood vessels. Collagen includes various types, from type I to type XII, of which type I collagen is the most common. [0003] Type I collagen is composed of two identical α1(I) peptide chains and a different α2(I) peptide chain, and the three peptide chains are wound into a special triple helical structure, which can be used as a scaffold for cell attachment and migration; and the peptide chain The arginine-glycine-aspartic acid (RGD) in the drug is the binding site of cell adhesion molecules (such as integrins), which can effectively promote cell proliferation and differentiation. [0004] Based...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/65A61K8/02A61K8/34A61K8/365A61K8/60A61Q19/00
CPCA61K8/65A61K8/345A61K8/60A61K8/365A61K8/022A61Q19/00A61K2800/10A61K2800/84
Inventor 唐小江张晓丽胡伟肖凌琥梁炤垣
Owner GUANGDONG ZHONGERJIAN BIOTECH CO LTD
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