The invention relates to a recomposed 
escherichia coli base 
cell for efficient synthesis of 
terpene chemical compounds as well as a preparation method and application thereof. Particularly, an 
escherichia coli endogenous 2-C-methyl-D-
erythritol-4-
phosphoric acid (MEP) precursor pathway is reconstructed; the reconstructed 
escherichia coli base 
cell is utilized to perform the efficient biological synthesis of the 
terpene chemical compounds; the reconstruction of the precursor pathway mainly comprises the steps of fully digging the MEP precursor pathway 
gene modules of the sources of other natural microorganisms, screening 
gene modules with excellent characteristics to be expressed in the 
escherichia coli, and at the same time, performing the downstream synthesis pathway for integrally assembling the colibacillus chemical compounds in the reconstructed base 
cell, wherein the colibacillus chemical compounds include 
sesquiterpene chemical compounds such as amorphadiene, 
diterpene chemical compounds such as shell 
alkene, tetraterpenes chemical compounds such as 
lycopene, polyterpene chemical compounds, other 
terpene alkaloid chemical compounds and the like. The 
escherichia coli base cell can remarkably facilitate the synthesis of the terpene chemical compounds.