Cytoskeleton for tissue-engineered tympanic membrane

A cell scaffold and membranous technology, applied in the field of membranous matrix materials and its preparation, can solve the problems of the construction and application of tissue engineered tympanic membranes that have not yet been seen

Inactive Publication Date: 2010-06-09
孙建军 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no research reports on the construction and application of tissue-engineered tympanic membranes in the true sense with cellular components

Method used

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  • Cytoskeleton for tissue-engineered tympanic membrane
  • Cytoskeleton for tissue-engineered tympanic membrane
  • Cytoskeleton for tissue-engineered tympanic membrane

Examples

Experimental program
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Effect test

Embodiment Construction

[0042] 1. Extraction of rat tail collagen: take the tail of an adult rat, remove the skin on the tail, separate the tendon from the muscle and other tissues; soak the tendon in 70% ethanol for 20 minutes, chop it up, and rinse it with deionized water; transfer to dilute In the acetic acid (1:1000) solution, use 250ml acetic acid solution for each rat tail, keep at 4°C for 48h; centrifuge at 4000r / min for 30min, take the supernatant; add 0.1mol / L NaOH, mix according to the volume ratio of 6:1, and neutralize the acetic acid , centrifuge at 1500r / min for 5min to obtain the precipitate.

[0043] 2. Prepare 2% acetic acid solution; respectively make collagen and 75% deacetylated chitosan into 1% dispersion; mix the two dispersions at a ratio of 9:1, stir at a constant speed for 2 hours at 4°C, Stir again with magnetic force for 5 hours; transfer to a centrifuge tube, balance, and centrifuge at 2000 rpm at 4°C for 5 minutes to remove the air bubbles in the dispersion; pour the prep...

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Abstract

The invention discloses a membranous matrix (cytoskeleton) which is prepared by mixing collagen and chitosan and then drying the mixture. The preparation process is simple. The collagen component is collagen I extracted from the rat tails, accounting for 90% of the weight of the mixture. The chitosan component is 75-95% of deacetylated chitosan, accounting for 10% of the weight of the mixture. The invention adopts the composite membrane as the cytoskeleton for constructing the tissue-engineered tympanic membrane and finds that the composite membrane is suitable for grow and proliferation of epithelial cell/ keratinocyte and fibroblast of the tympanic membrane and can maintain good collagen secretion function of the fibroblast. The cytoskeleton further lays the foundation for constructing the tissue-engineered tympanic membrane.

Description

technical field [0001] The present invention relates to a kind of membranous matrix (cell scaffold) material and preparation method thereof for constructing tissue-engineered tympanic membrane, more specifically, the present invention relates to the preparation of a kind of new collagen-chitosan membrane, utilize this material to be able to in vitro Inoculate keratinocytes and fibroblasts to provide cell scaffolds for the construction of tissue-engineered tympanic membranes. Background technique [0002] The tympanic membrane is one of the important sound-transmitting structures in the auditory organ. It increases the sound pressure acting on the tympanic membrane to the oval window membrane by 17 times. In addition, the complete tympanic membrane-ossicle chain sound transmission system can also ensure that the sound waves The one-way sound transmission function of the oval window improves the hearing sensitivity. The tympanic membrane is a membranous structure with a thick...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/20A61F2/82
Inventor 孙建军汤勇
Owner 孙建军
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