Device and method for patterning co-culture of multiple cells

Abstract

The invention provides a device and method for patterning co-culture of multiple cells. Adhesive tape is punched through a punching needle to form an array with micrometer-order holes, the array is used as a template, polydimethylsiloxane is directly poured to the template to obtain a channel with micrometer-order pillar array inside, polydimethylsiloxane is printed on a cell petri dish or culture plate base, the surface of the cell petri dish or culture plate base is not processed, or a rat tail collagen layer is incubated on the surface to form the culture device. The method of cell culture through the device is provided, the device can be manufactured just by the adhesive tape, the punching needle, a nicking tool and polydimethylsiloxane without photolithography, the device can be used for patterning any kind of cells, co-culture of the multiple cells is achieved, and limitation to photolithography by traditional micro-fluidic chip preparation is broken through.

Description

technical field [0001] The present invention relates to cell culture technology, in particular, the present invention relates to a device and method capable of patterning and co-cultivating a variety of cells on an adhesive tape-based microfluidic chip. Background technique [0002] Beginning in the 1970s, patterning techniques for biomolecules and cells began to emerge. Cell patterning technology is mainly divided into two categories: one is to form a patterned area for cell adhesion and growth through surface modification, so that cells can selectively adhere and grow to form a pattern; the other is to restrict cells to The patterned regions grow, forming cell patterns. Based on the above two principles, various cell patterning techniques have emerged and developed. [0003] Currently, cell patterning methods mainly include photolithography and soft etching. Photolithography, originally used in the semiconductor industry, uses ultraviolet or white light to transfer geom...

AI Technical Summary

Problems solved by technology

Since microfluidic patterning uses solutions of different molecules to form patterns, generally speaking, the patterns of each molecule are continuously distributed on the surface, which limits the diversity of pattern shapes.

Template patterning is a method for cell patterning without chemical modification of the substrate, but this method is relatively easy to produce simple patterns such as circles and squares, and patterns with many corners are easily damaged during the peeling process of PDMS

More importantly, molds for making microfluidic chips are difficult to obtain in ordinary biological laboratories, making it difficult to use microfluidic chips for cell migration and cell patterning research

[0005] It can be seen that the disadvantage of the current cell patterning method is that the operation process has high requirements on the environment, equipment and experimenters, and the reliance on photolithography to produce molds limits the application of microfluidic chips for cell patterning experiments in biological laboratories.

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