Method for inducing bionic calcification in collagen fibers through polymer polyelectrolyte, and applications thereof

A polyelectrolyte and collagen fiber technology, applied in tissue regeneration, pharmaceutical formulation, medical science, etc., to achieve the effects of enhanced mechanical strength, strong modifiability, and good internal environment

Active Publication Date: 2017-10-03
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the defects or deficiencies of existing bone defect repair materials, the object of the present invention is to provide two types of polymer polyelectrolytes—polycations and polyanions to

Method used

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  • Method for inducing bionic calcification in collagen fibers through polymer polyelectrolyte, and applications thereof
  • Method for inducing bionic calcification in collagen fibers through polymer polyelectrolyte, and applications thereof
  • Method for inducing bionic calcification in collagen fibers through polymer polyelectrolyte, and applications thereof

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Embodiment 1

[0044] (1) Prepare 9mM CaCl with 10mM HEPES buffer as solvent 2 2H 2 O solution and 4.2mM K 2 HPO 4 solution, the above solutions were mixed isometrically to prepare a calcium phosphate solution (final concentration: 4.5mM CaCl 2 2H 2 O / 2.1mM K 2 HPO 4 );

[0045] (2) Polypropylene ammonium chloride (average molecular weight 150kDa) was dissolved in calcium phosphate solution at a ratio of 200ug / mL, and polypropylene ammonium chloride was prepared by utilizing the stabilizing effect of polypropylene ammonium chloride on supersaturated calcium phosphate solution Stable amorphous liquid phase calcium phosphate precursor solution.

[0046] (3) Put the collagen sponge or the completely demineralized bone collagen matrix in the calcium phosphate mineralization solution stabilized by polypropylene ammonium chloride, incubate at a constant temperature of 37°C for 7 days, and replace it with fresh polypropylene ammonium chloride stabilized solution every day. The calcium phosp...

Embodiment 2

[0060] This embodiment differs from Embodiment 1 in that:

[0061](2) Polypropylene ammonium chloride (average molecular weight 150kDa) was dissolved in calcium phosphate solution at a ratio of 400 μg / mL, and polypropylene ammonium chloride was obtained by utilizing the stabilizing effect of polypropylene ammonium chloride on supersaturated calcium phosphate solution Stable amorphous liquid phase calcium phosphate precursor solution.

[0062] (3) Place the completely demineralized bone collagen in calcium phosphate mineralization solution stabilized by polypropylene ammonium chloride, incubate at a constant temperature of 37°C for 14 days, and replace with fresh calcium phosphate mineralization solution stabilized by polypropylene ammonium chloride every day , rinsed repeatedly with distilled water, and dried for later use, to prepare a biomimetic calcified bone collagen scaffold material within collagen fibers.

Embodiment 3

[0064] This embodiment differs from Embodiment 1 in that:

[0065] (2) Dissolve polypropylene ammonium chloride (average molecular weight 150kDa) in calcium phosphate solution at a ratio of 800 μg / mL, and use polypropylene ammonium chloride to stabilize the supersaturated calcium phosphate solution to obtain polypropylene ammonium chloride Stable amorphous liquid phase calcium phosphate precursor solution.

[0066] (3) Place the completely demineralized bone collagen in calcium phosphate mineralization solution stabilized by polypropylene ammonium chloride, incubate at a constant temperature of 37°C for 14 days, and replace with fresh calcium phosphate mineralization solution stabilized by polypropylene ammonium chloride every day , rinsed repeatedly with distilled water, and dried for later use, to prepare a biomimetic calcified bone collagen scaffold material within collagen fibers.

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Abstract

The present invention relates to a method for inducing the bionic calcification in collagen fibers through a polymer polyelectrolyte, and applications thereof. The method comprises: treating different types of collagen scaffolds in a calcium phosphate mineralized solution containing a cationic polyelectrolyte or anionic polyelectrolyte to obtain the bionic calcification collagen scaffold material having the inside-fiber mineralization characteristic, wherein the collagen scaffold is recombinant collagens type I having different sources, completely demineralized bone collagen, completely demineralized dentin collagen, or rat tail collagen, and the mineralization liquid is a calcium phosphate solution added with a cationic polyelectrolyte or anionic polyelectrolyte. According to the present invention, through the stabilizing effect of the polyelectrolyte on the calcium phosphate solution, the novel bionic calcified collagen scaffold material with characteristics of similar natural bone tissue structure, good mechanical property, good biocompatibility and suitable biodegradability is constructed.

Description

technical field [0001] The invention belongs to the field of biomimetic mineralization materials and tissue engineering scaffold materials, and relates to a method for inducing biomimetic calcification in collagen fibers by a polymer polyelectrolyte and an application thereof. Background technique [0002] In today's society, with the increasing number of bone tissue defects caused by aging population, trauma, tumors and other reasons, people's demand for bone graft materials is also increasing. Among the existing bone substitute materials, autologous bone and allogeneic bone are commonly used transplant materials, but they generally have limited sources, complicated surgical operations, immune rejection, long bone healing time, low healing rate, poor tolerance and many complications. Therefore, the synthesis of highly biomimetic artificial bone regeneration repair materials with structure and function is the key to solve this problem. [0003] Natural bone tissue is mainly...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/58A61L27/12
CPCA61L27/12A61L27/24A61L27/58A61L2400/12A61L2430/02A61L2430/12
Inventor 牛丽娜焦凯陈吉华宋群郑智明
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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