Preservation solution and preparation method and application thereof

Abstract

The invention provides a feces preservation solution, a preparation method and an application thereof and a feces preservation method, and belongs to the technical field of biological agents. The feces preservation solution is prepared from the following components: a chelating agent with concentration of 10-500 mmol / L, a bacteriostat with concentration of 100-600 mL / L, an ionic strength maintainer with concentration of 1-10 g / L and a denaturant with concentration of 1-50 g / L. The feces preservation solution can preserve feces samples at normal temperature, and can effectively inhibit DNA degradation and cell damage in feces samples and maintain cell morphology, and has better effect of inhibiting activity of degrading enzyme and microorganisms, and preservation time of the samples is long.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, and in particular relates to a feces preservation solution, a preparation method and application thereof, and a feces preservation method. Background technique [0002] In recent years, the detection of exfoliated cells in human feces is often used in the primary screening of colorectal cancer and other diseases due to its non-invasive, painless and high specificity characteristics. It can better achieve the purpose of "early detection, early diagnosis and early treatment". [0003] However, the composition of feces is very complex, containing a large amount of digestive residues and various nucleic acid degrading enzymes produced by bacteria such as proteases, DNases (DNases) and RNases (RNases), various PCR inhibitors (such as bile salts, bilirubin, humic substances, etc.) and pigments), making cells and nucleic acids in stool samples extremely vulnerable to damage and degradat...

AI Technical Summary

Problems solved by technology

However, the collection of stool samples is often not carried out in the laboratory, that is to say, the collected stool samples more or less need to be placed at room temperature for a period of time before they can be stored in the refrigerator at -20°C; A small amount of exfoliated cells is further lost, which affects subsequent detection

[0004] The current method of preservation of feces samples has a short storage time, a low pass rate of sample re-use, harsh storage conditions, and high costs.

Therefore, the current stool sample liquid and preservation methods still need to be improved.

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