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Zaralenone degrading bacteria and application thereof

A technology of zearalenone, degrading bacteria, applied in application, bacteria, food science, etc.

Inactive Publication Date: 2016-10-26
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A strain of Bacillus amyloliquefaciens CGMCC NO.8726 disclosed in the invention patent with publication number CN10398113A also has a good degradation effect on ZEN, with a degradation rate of 95.99±0.23%, but this strain can only degrade 5 μg / mL of ZEN

Method used

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  • Zaralenone degrading bacteria and application thereof
  • Zaralenone degrading bacteria and application thereof
  • Zaralenone degrading bacteria and application thereof

Examples

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Effect test

Embodiment 1

[0026] Application of zearalenone-degrading bacteria (CCTCC NO:M2016129) in degrading zearalenone.

Embodiment 2

[0028] Application of zearalenone-degrading bacteria (CCTCC NO: M2016129) in the preparation of microbial preparations for degrading zearalenone.

Embodiment 3

[0030] Microbial preparations, including zearalenone-degrading bacteria (CCTCC NO:M2016129).

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Abstract

The invention discloses zaralenone degrading bacteria and application thereof, and belongs to the technical field of biology. ZEN is used as a unique carbon source and energy to carry out preliminary screening and secondary screening, a bacteria strain H6 (CCTCC NO: M2016129) capable of degrading ZEN effectively is separated out, fermentation liquor of the bacteria H6 and ZEN (with final concentration of 10 (mu)g / mL) are cultured for 72 hours under the conditions of 37 DEG C and 180 r / min, and the degradation rate to ZEN reaches 93.64%. Through cellular morphology and physiological and biochemical identification and 16 S rRNA sequence comparative analysis, the strain of bacteria is identified as the bacillus amyloliquefaciens. The degradation active substances of the bacteria strain H6 mainly exist in fermented supernate, thalli also have partial degradation effect. The bacteria strain H6 can be used for preparing a microorganism preparation for degrading zaralenone or a feed biodegradation additive.

Description

technical field [0001] The invention relates to a zearalenone-degrading bacterium, and also relates to the application of the degrading bacterium, belonging to the field of biotechnology. Background technique [0002] Zearalenone (Zearalenone, ZEN), also known as F-2 toxin, is a phenolic dihydroxybenzoic acid lactone structure similar to estradiol, mainly produced by Fusarium graminearum with estrogen-like properties The fungal toxins that have the effect of zearalenone can cause great harm to the health of humans and animals, and can make the body continuously in estrus and lead to a series of reproductive disorders such as pseudopregnancy, infertility, ovarian deformity and abortion. In addition, studies have confirmed that zearalenone also has Genotoxicity, cytotoxicity, immunotoxicity and carcinogenicity. At present, ZEN pollution exists in grains and agricultural by-products in many countries in the world, causing huge economic losses to livestock farms or food industr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23L5/20C12R1/07
CPCC12N1/205C12R2001/07
Inventor 王彦彬孙向丽魏单平康相涛刘小军孙桂荣
Owner HENAN AGRICULTURAL UNIVERSITY
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