Vector capable of promoting self renewal and proliferation of spermatogonial stem cells of dairy goat and application of vector

A technology of spermatogonial stem cells and cell proliferation, which is applied in the field of self-renewal and proliferation carriers, can solve the problems of adult tissue stem cells, etc., and achieve the effect of increasing self-renewal ability, accelerating cell proliferation, and promoting self-renewal and proliferation
CN104404083AInactive Publication Date: 2015-03-11NORTHWEST A & F UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
NORTHWEST A & F UNIV
Publication Date
2015-03-11
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a vector capable of promoting the self renewal and proliferation of spermatogonial stem cells of a dairy goat and application of the vector. The vector is an eukaryotic expression vector containing a dairy goat gSirt1 gene, wherein the nucleotide sequence of the dairy goat gSirt1 gene is shown as SEQ.ID.NO.1. According to the vector and the application of the vector, over-expression or super expression in the spermatogonial stem cells is realized by the constructed eukaryotic expression vector, and RT-PCR (Reverse Transcription-Polymerase Chain Reaction), Western blot and cell cycle examination show that the gSirt1 gene is helpful for the self renewal, stemness (pluripotency) maintenance and cell proliferation of the spermatogonial stem cells.
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Description

technical field

[0001] The invention belongs to the technical field of spermatogonial stem cells, and relates to a carrier for promoting self-renewal and proliferation of dairy goat spermatogonial stem cells and an application thereof. Background technique

[0002] Spermatogonial stem cells (SSCs), also known as male germline stem cells (mGSCs), are a type of adult stem cells located on the basement membrane of the seminiferous tubules of the testis. SSCs can maintain their own population through self-renewal The number is stable, and can develop and differentiate to form sperm cells. Studies have shown that in the microenvironment of the testis, SSCs, like embryonic stem cells, have the potential to differentiate into all cell types including the three germ layers in vitro and in vivo (Oatley JM, Brinster RL. Regulation of spermatogonial stem cell self-renewal in mammals. Annu Rev Cell Dev Biol 2008;24:263-86.). Spermatogonia stem cells can be used as an ideal in vitro mo...

Claims

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