A serum-free medium for monkey embryonic stem cells

A technology of serum-free medium and embryonic stem cells, which is applied in the field of stem cell culture to achieve the effects of ensuring safety, eliminating pathogen contamination, and determining components
CN106754657BActive Publication Date: 2022-07-22北京赛斯达生物技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
北京赛斯达生物技术有限公司
Publication Date
2022-07-22

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Abstract

The invention discloses a serum-free medium for monkey embryonic stem cells, comprising a basal medium and additives dissolved in the basal medium, wherein: the basal medium comprises DMEM-F12 medium, and the supplements include L - Glutamine, non-essential amino acids, L-ascorbic acid, sodium selenite, β-mercaptoethanol, human serum albumin, sodium heparin, human transferrin, human insulin, basic fibroblast growth factor, transforming growth factor β1 and epidermal growth factor. The medium can maintain the undifferentiated state and totipotency of monkey embryonic stem cells for a long time, and is suitable for culturing monkey embryonic stem cells in a feeder-free system and for culturing monkey embryonic stem cells in a feeder layer system.
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Description

technical field

[0001] The invention relates to the field of stem cell culture, in particular to a serum-free medium for monkey embryonic stem cells. Background technique

[0002] Embryonic stem cells (ESCs) are highly undifferentiated cells isolated from the inner cell mass (ICM) or primordial germ cells (PGCs) of early embryos and can be cultured in vitro. , which can differentiate into permanent cell lines of various tissues and cell types derived from the 3 germ layers. It is generally believed that embryonic stem cells will have an important impact on in vitro research on animal and human embryogenesis and development, new gene discovery, drug screening and other fields.

[0003] Although human embryonic stem cells (hESCs) have important research and application value in the fields of regenerative medicine, drug screening and developmental biology, the use of human embryonic stem cells and primary embryos mainly involves ethical issues. So far, many countries have ena...

Claims

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