Reagent for interfering with expression of hsa-mir-449a and application thereof

A technology of hsa-mir-449a and sh-mir-449a, which is applied in the field of genetic engineering, can solve the problems that have not been clearly reported and the regulatory mechanism of tumor stem cells is unclear, so as to delay the growth of tumors in vivo, inhibit self-renewal, and inhibit proliferation Effect
CN107723289BActive Publication Date: 2020-08-11CHONGQING PRECISION BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHONGQING PRECISION BIOTECH CO LTD
Publication Date
2020-08-11

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Abstract

The invention belongs to the field of genetic engineering, and relates to a reagent for interfering with expression of Hsa-miR-449a and application of the reagent and mainly relates to the applicationof the reagent in preparing medicine for inhibiting self-renewal of liver cancer stem cells. The nucleotide sequence of a cancer gene Hsa-miR-449a for promoting the self-renewal of the liver cancer stem cells is shown in SEQ ID NO:1. The invention discloses the expression of Hsa-miR-449a in the liver cancer stem cells for the first time, and the expression is crucial to maintaining the self-renewal of the liver cancer stem cells. The application of the Hsa-miR-449a gene in inhibiting the self-renewal of the liver cancer stem cells is to inhibit the self-renewal of the liver cancer stem cellsby interfering with the expression of Hsa-miR-449a in the liver cancer stem cells. The reagent for interfering with the expression of Hsa-mi-449a can be prepared into the medicine for inhibiting the self-renewal of the liver cancer stem cells, is used for targeted treatment of liver cancer and has an important significance in prolonging the survival time of a liver cancer patient.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering, and relates to a reagent for interfering with the expression of Hsa-miR-449a and its application, in particular to the application in the preparation of a drug for inhibiting the self-renewal of liver cancer stem cells. Background technique

[0002] Liver cancer is one of the most common malignant tumors in the world. Its pathogenesis has not yet been clarified. The current treatment of liver cancer is not ideal. Postoperative recurrence and metastasis are still a major problem to be solved. Recent studies have made the concept of cancer stem cells (CSCs) deeply rooted in the hearts of the people, and more and more evidences show that CSCs are closely related to tumor growth, drug resistance, recurrence and metastasis. So far, the existence of tumor stem cells has been confirmed in more than 30 common tumors such as blood system tumors, glioma, liver cancer, breast cancer, and colorectal cancer...

Claims

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