The invention relates to the medical field, and discloses a culture medium for neural stem cell proliferation and capable of inducing neural stem cell to differentiate to dopaminergic neuron and an application and proliferation inducing method. The proliferation culture medium is a culture medium using DMEM/F12 as the base. The culture medium comprises insulin human, progesterone, hydrocortisone, dexamethasone, estradiol, transferrin, EGF, FGF, CTGF, B-NGF, VEGF, a soybean pancreatin inhibitor, L-glutathione, oleic acid, Tween 80, phosphatidic acid, sodium selenite, panax notoginseng saponins, resveratrol, lithium chloride, cholesterol, WNT3a, and glutamine. A novel neural stem cell culture medium is formed by using a plurality of suitable components with low cost and extensive sources, the proliferation speed of the neural stem cells is remarkably improved, and other induced differentiation components are added on the basis of the neural stem cell, so the neural stem cell is induced to be differentiated to the dopaminergic neuron, and the differentiation proportion is remarkably improved.