Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cell culture medium

a cell culture medium and cell technology, applied in the field of cell culture medium and culture media, can solve the problems of reducing lactate accumulation, which may become toxic to cells, and achieve the effect of increasing protein yield

Inactive Publication Date: 2019-02-21
AGENCY FOR SCI TECH & RES
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent text describes a serum-free cell culture medium that uses maltose as the main carbohydrates source. The medium can also contain other saccharides. This medium is suitable for growing and culturing cells without the need for serum. The technical effects of this invention include increased protein yield and improved cell growth and culture. A kit containing the components of this medium is also provided.

Problems solved by technology

In particular, the potential use of alternative carbohydrate source may lead to the improvement of carbohydrate loading in the batch culture medium, and possibly lead to the decrease lactate accumulation which may become toxic to cells.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell culture medium
  • Cell culture medium
  • Cell culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027]Most cultured cells (such as mammalian cells) are chemoheterotrophic and typically require a carbohydrate source for growth in cultures. As carbohydrates have low permeability through the phospholipid bilayer that makes the bulk of the cell membrane (Bresseleers et al., 1984; Wood et al., 1968), sugar transport into the cell is facilitated by transporter proteins (Jones and Nickson, 1982; Thorens, 1996; Wright et al., 1994). Hence, for the cultivation of cells (such as mammalian cells), glucose is the single most commonly used carbohydrate, because it can be efficiently transported into the cells through two major families of monosaccharide transporters, the sodium-glucose linked transporters (SGLT) (Wright et al., 1994) and glucose transporters (GLUT).

[0028]In addition to glucose, other carbohydrate sources have been tested for their ability to support growth of animal cell cultures (Altamirano et al., 2000; Morgan and Faik, 1981). In these reports, monosaccharides galactose,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
densityaaaaaaaaaa
concentrationsaaaaaaaaaa
Login to View More

Abstract

The invention relates to serum-free cell culture medium, wherein the medium either contains maltose as the sole carbohydrate source, or contains maltose and at least one other saccharide as carbohydrate sources. In a preferred embodiment, the additional saccharide is a monosaccharide, preferably glucose, and the medium comprises DMEM-F12. In a preferred embodiment the cells to be cultured may be CHO or HEK293 cells. Also disclosed are methods of growing and / or culturing a cell using the cell culture medium as described herein, methods of increasing protein yield using the cell culture medium as described herein, and kits thereof.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims the benefit of priority of Singapore patent application No. 10201601284Y, filed 22 Feb. 2016, the contents of it being hereby incorporated by reference in its entirety for all purposes.FIELD OF THE INVENTION[0002]The present invention relates to compositions for propagation, preservation, or maintenance of cells. In particular, the present invention relates to culture media. The present invention also relates to compositions for supporting protein production by cultured cells.BACKGROUND OF THE INVENTION[0003]From the first day of its inception, cell culture medium has progressively been improved and researched depending on the intention of the culture. The first few cell culture solution provides irrigation, supply of water, inorganic anions required for cell metabolism, osmotic balance, a buffering system, and a carbohydrate source for growth.[0004]As research into how cells function improves, cell culture medium ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12N5/00
CPCC12N5/0037C12N2500/90C12N2500/34C12N5/0031
Inventor NG, SAY KONGLEONG, SOW ZONG DAWNTAN, GEK LING JANICETEO, KAH HUL BRIAN
Owner AGENCY FOR SCI TECH & RES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com