Cell culture medium

a cell culture medium and cell technology, applied in the field of cell culture medium and culture media, can solve the problems of reducing lactate accumulation, which may become toxic to cells, and achieve the effect of increasing protein yield
US20190055513A1Inactive Publication Date: 2019-02-21AGENCY FOR SCI TECH & RES
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
AGENCY FOR SCI TECH & RES
Publication Date
2019-02-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to serum-free cell culture medium, wherein the medium either contains maltose as the sole carbohydrate source, or contains maltose and at least one other saccharide as carbohydrate sources. In a preferred embodiment, the additional saccharide is a monosaccharide, preferably glucose, and the medium comprises DMEM-F12. In a preferred embodiment the cells to be cultured may be CHO or HEK293 cells. Also disclosed are methods of growing and / or culturing a cell using the cell culture medium as described herein, methods of increasing protein yield using the cell culture medium as described herein, and kits thereof.
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Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application claims the benefit of priority of Singapore patent application No. 10201601284Y, filed 22 Feb. 2016, the contents of it being hereby incorporated by reference in its entirety for all purposes.FIELD OF THE INVENTION

[0002] The present invention relates to compositions for propagation, preservation, or maintenance of cells. In particular, the present invention relates to culture media. The present invention also relates to compositions for supporting protein production by cultured cells.BACKGROUND OF THE INVENTION

[0003] From the first day of its inception, cell culture medium has progressively been improved and researched depending on the intention of the culture. The first few cell culture solution provides irrigation, supply of water, inorganic anions required for cell metabolism, osmotic balance, a buffering system, and a carbohydrate source for growth.

[0004] As research into how cells function improves, cell culture medium ...

Claims

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