Mesenchymal stem cell serum-free culture medium

A serum-free medium and stem cell technology, applied in the field of mesenchymal stem cell serum-free medium, can solve the problem that the content of components and specific functions are not completely determined, etc.
CN106190964AActive Publication Date: 2016-12-07GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
Publication Date
2016-12-07

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Abstract

The invention discloses a mesenchymal stem cell serum-free culture medium. The culture medium takes an L-DMEM, DMEM-F12 or alpha-MEM culture medium as a basic culture medium and also includes the following addition components: fibronectin, laminin, transferrin, trypsin, aprotinin, growth hormone, insulin, hydrocortisone, dexamethasone, bFGF, EGF, B27, IGF-I, IGF-II, choline, linoleic acid, sodium selenite, phosphorylethanolamine, glutathione, vitamin C, vitamin E, vitamin B12 and biotin. The culture medium has the advantages of defined composition and controllable quality, and mesenchymal stem cells cultured by the culture medium grow normal; and after multiple passages, the 'dryness' of the mesenchymal stem cells and the ability of the mesenchymal stem cells to be differentiated into osteoblasts, chondrocytes, adipocytes, neurons and other types of cells are still kept.
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Description

technical field

[0001] The invention belongs to the field of biomedicine, and in particular relates to a serum-free medium for mesenchymal stem cells. Background technique

[0002] Mesenchymal stem cells (MSCs) are pluripotent stem cells derived from mesoderm with high self-renewal ability and multi-directional differentiation potential. It is isolated and can be differentiated into osteoblasts, chondrocytes, adipocytes and neurons under specific conditions. With a wide range of sources and no ethical and moral issues, MSC has been proven in many ways to be an ideal seed cell for autologous and allogeneic cell transplantation and treatment. At present, a number of clinical application studies of MSCs have been carried out at home and abroad, involving various types of MSCs from various sources and various types of diseases.

[0003] The medium components used in the preparation of clinically applied stem cell preparations should have sufficient purity and meet the quality ...

Claims

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