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Connexin core sequence-containing amphiphilic polypeptide and application thereof

A core sequence, linking protein technology, applied in the medical and biological fields, can solve the problems of high mechanical strength, immunogenicity, and toxicity of degradants

Inactive Publication Date: 2011-07-06
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although traditional scaffold materials such as chitosan, hyaluronic acid, fibrin, and collagen derivatives can promote cell adhesion, growth, and differentiation, they generally have high mechanical strength, poor biodegradability, and degradation products. It has the disadvantages of toxicity, poor biological activity and immunogenicity, which limit its application in intervertebral disc tissue engineering

Method used

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  • Connexin core sequence-containing amphiphilic polypeptide and application thereof
  • Connexin core sequence-containing amphiphilic polypeptide and application thereof
  • Connexin core sequence-containing amphiphilic polypeptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation of nanoscale fiber material of the present invention

[0045] The nanoscale fiber material of the present invention has a structure of AcN-RADARADARADARADAGGDHLSDNYTLDHDRAIH-CONH 2 (DHL-PA) Amphiphilic Peptide with structure AcN-RADARADARADARADA-CONH 2 (RADA-16-PA) is formed by self-assembly of amphiphilic polypeptides.

[0046] DHL-PA (AcN-RADARADARADARADAGGDHLSDNYTLDHDRAIH-CONH 2 ) amphiphilic polypeptide, the purity of the polypeptide detected by high performance liquid chromatography is 97.02%, and its molecular weight detected by mass spectrometer is 3730.92. For detailed parameters, see Figure 10 , 11 .

[0047] The concentration is 0.25%-10% (m / v) DHL-PA and RADA-16-PA amphipathic polypeptide solution according to the volume ratio of DHL-PA and (DHL-PA+RADA-16-PA) is 10%- 75% proportion in Ca 2+ Triggered self-assembly to form a composite nanofiber material (DHL-PA / RADA-16-PA). The composite material has good material-cell interface compatibili...

Embodiment 2

[0050] The difference from Example 1 is that the trigger condition for the self-assembly of DHL-PA and RADA-16-PA amphiphilic polypeptide solutions to form composite nanofibrous materials is: self-assembly under the trigger of cell culture medium DMEM-F12 to form composite nanofibers fiber material. All the other are with embodiment 1.

[0051] The following is the sequence list of amino acids involved in the present invention, wherein: Sequence 1 is: link protein core sequence linkN; Sequence 2 is: RADA-16 evolved from the EAKA16-II fragment of zuotin yeast protein; Sequence 3 is: provided by the present invention An amphipathic polypeptide containing a connexin core sequence.

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Abstract

The invention provides connexin core sequence-containing amphiphilic polypeptide AcN-RADARADARADARADAGGDHLSDNYTLDHDRAIH-CONH2, wherein the amphiphilic polypeptide and the amphiphilic polypeptide with the structure of AcN-RADARADARADARADA-CONH2 are triggered by Ca2+or a cell culture medium DMEM-F12 to automatically form into a compound, i.e. a nanometer-grade fiber material, and the nanometer-grade fiber material is taken as a bracket of the bone mesenchymal stem cells, so that the biological behavior of the cells can be obviously improved, the degenerated intervertebral disc can be repaired, and the original height can be recovered.

Description

Technical field: [0001] The invention belongs to medicine and biotechnology, and relates to an amphipathic polypeptide containing a connexin core sequence and its application in medicine. Background technique: [0002] Intervertebral disc degeneration (IVD) is the main cause of low back pain, research shows that 60%-80% of people will have low back pain at least once in their life. The degeneration of the intervertebral disc is mainly manifested by the reduction of the water content of the nucleus pulposus, which reduces the tension of the nucleus pulposus and the height of the intervertebral disc. At the same time, hyaluronic acid and keratin sulfate decreased, low-molecular-weight glycoproteins increased, fibrillar degeneration and collagen fiber deposition increased, nucleus pulposus lost elasticity, and the intervertebral disc structure relaxed. Once this degeneration occurs, it will fall into a vicious circle, which will further aggravate the degeneration of the interv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00A61L27/22D01F8/02
Inventor 邵增务吴永超王佰川杨述华张志才
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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