Compositions and methods to culturing neural stem cells with bone marrow stromal cells

A technology of bone marrow stromal cells and neural stem cells, which can be used in bone/connective tissue cells, animal cells, tissue culture, etc., and can solve problems such as dangerous side effects
CN1961068AInactive Publication Date: 2007-05-09THERADIGM INC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THERADIGM INC
Publication Date
2007-05-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention encompasses methods and compositions for enhancing the growth of neural stem cells. Methods for modulating MHC molecule expression on a neural stem cell (NSC) are also included in the invention. Figure 1 is a graph depicting the proliferation of BMSCs in BMSC medium (DMEM-low glucose, 10% lot tested fetal bovine serum) or NSC medium (DMEM-F12, N2-Supplement, EGF 20 ng / ml, bFGF 10 ng / ml, heparin 8 ug / ml, penicillin-streptomycin).
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Description

Background technique

[0001] Bone marrow includes at least two types of stem cells: hematopoietic stem cells and non-hematopoietic tissue stem cells, the latter referred to variously: mesenchymal stem cells, spinal cord stromal cells (MSCs) or bone marrow stromal cells (BMSCs), these terms are used in this document synonymous. Spinal stromal cells are of interest because they can be easily isolated from small bone marrow aspirate and readily generate single-cell-derived colonies. Single cell-derived colonies can have 50 population doublings in 10 weeks and differentiate into osteoblasts, adipocytes, chondrocytes (A.J.Friedenstein et al. 1970 Cell Tissue Kinet.3:393-403; H.Castro-Malaspina et al. 1980 Blood 56:289-301; N.N.Beresford et al. 1992 J.Cell Sci.102:341-351; D.J.Prockop 1997 Science 276:71-74), muscle cells (S.Wakitani et al. 1995 Muscle Nerve 18:1417-1426), astrocytes, oligodendrocytes and neurons (S.A.Azizi et al. 1998 Proc.Natl.Acad.Sci.USA 95:3908-3913; G.C.Kopen...

Claims

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