49results about How to "Successful separation" patented technology

The invention relates to a method for separating and amplifying a mesenchymal stem cell from a fresh tissue of an umbilical cord. The method comprises the following steps of: sterilizing and cleaning an umbilical cord tissue; shearing the tissue into a block shape; carrying out digestion treatment for 1-2.5 hours and ending the digestion; cleaning the cell obtained by digesting to obtain a cell suspension; adding the cell suspension into a T25 cell culture bottle for culturing; supplementing after culturing the cell suspension for 3-6 days and continuously culturing; carrying out full liquid change for the first time when the cell suspension is cultured for 8-10 days and then carrying out one full medium change every one to three days; when the fusion rate of anchorage-dependent cells in a plate reaches about 50-70 percent, enabling the anchorage-dependent cells to be separated from the bottom of the T25 cell culture bottle by using digestive enzyme; removing supernate by centrifuging, adding a mesenchymal stem cell culture medium for re-suspending the cell, inoculating the cell to the T25 cell culture bottle for carrying out passage and amplifying culture; and then changing liquid once every 1-3 days until the fusion rate reaches 70-90 percent and obtaining the umbilical cord mesenchymal stem cell. The method disclosed by the invention can be effectively used for separating and augmenting the mesenchymal stem cell.

The invention relates to a method for preparing CD34 positive cells from umbilical cord mesenchymal stem cells. The method specifically comprises the following steps: (a) providing mesenchymal stem cells from an umbilical cord; (b) performing primary culture on the mesenchymal stem cells; (c) performing subculture on the mesenchymal stem cells; (d) in a subculture process, adding an inductive agent in a culture medium for inducing the mesenchymal stem cells; (e) obtaining the CD34 positive cells. The invention also relates to cell products of the CD34 positive cells, prepared through the method and application of the cell products. The method disclosed by the invention has excellent technical effects described in the description.

The invention relates to canine umbilical cord mesenchymal stem cells as well as a preparation method and a cryopreservation method thereof. Specifically, the method comprises the following steps: disinfection and washing, digestion treatment, cell culture, cell passage, and cell cryopreservation. The method for preparing mesenchymal stem cells from a canine umbilical cord exhibits an excellent technical effect. The mesenchymal stem cells is beneficial for treatment of canine arthritis, fractures, muscle damage, ligament injury, cartilage damage, joint damage, cognitive dysfunction, immune-mediated diseases, dry eye, recurrence uveitis, liver disease, heart disease, kidney disease, diabetes, gastrointestinal disease, thyroid disease and skin disease.

Provided is a method for separating and extracting mesenchymal stem cells from the human umbilical cord. The method uses healthy neonatal umbilical cord tissue; after cleaning and disinfection, mechanically pulverising same, separating the Wharton's jelly, and after treating with erythrocyte lysate, carrying out suspension culture in a serum-free culture medium. Replacing the liquid every 3-5 days; after the plate adherence rate reaches 30-70%, carrying out trypsin digestion, and then collecting the cells by centrifugation for passage amplification, until the rate of confluence of the cells reaches 80-90% confluence, thereby obtaining high purity umbilical cord mesenchymal stem cells.

The invention relates to a method for preparing mesenchymal stem cells from the umbilical cord of a dog. Specifically, the method comprises the following steps: sterilization and cleaning, digestion treatment, cell culture, cell passage and cell cryopreservation. The method for preparing mesenchymal stem cells from the umbilical cord of the dog, which is provided by the invention, has excellent technical effects; the obtained mesenchymal stem cells are beneficial to treatment on arthritis, bone fracture, muscle injury, ligamentous injury, cartilage injury, joint injury, cognition impairment, immune-mediated diseases, xerophthalmia, recurrent uveitis, hepatic diseases, heart diseases, kidney diseases, diabetes, gastrointestinal diseases, thyroid diseases and skin diseases of canidae.

The invention discloses a method for separating and recycling vanadium and chromium from chromium vanadium reducing slag. The method comprises the following steps that (1) the chromium vanadium reducing slag and oxidability chromium salt are roasted, wherein the roasting condition comprises the temperature of 300 DEG C to 400 DEG C and the time of 60 minutes to 240 minutes; (2) water leaching is carried out on roasted materials in the step (1), then, solid-liquid separation is carried out on the materials, and lixivium containing the vanadium is obtained; (3) acid leaching and/or alkaline leaching are/is carried out on a solid phase obtained after solid-liquid separation in the step (2), then solid-liquid separation is carried out on the solid phase, and lixivium containing the chromium is obtained. Through the method, the vanadium in the chromium vanadium reducing slag can be directly oxidized at the low roasting temperature, the vanadium can be extracted through following water leaching, the chromium is extracted through following acid leaching and/or alkaline leaching, and the vanadium and the chromium in the chromium vanadium reducing slag can be successfully separated.

The invention discloses a freestone type peach pitting device. The freestone type peach pitting device comprises a device support, a transmission mechanism, a valve opening mechanism and a control circuit board, wherein the transmission mechanism comprises a driving motor, a driving chain wheel and a driven chain well, wherein the driving chain wheel and the driven chain wheel are correspondingly arranged on the upper portion of the device support and provided with two transmission chains in a sleeved mode; the driving motor is arranged under the device support and connected with the driving chain wheel in a chain transmission mode; the valve opening mechanism comprises a pressing device, a stone valve separating mechanism, a detecting and positioning unit and at least one group of initial positioning unit. By means of the initial positioning unit, the pressing device and the stone valve separating mechanism, the freestone type peach pitting device can easily achieve separation of peach stones and pulp, thereby effectively solving the problems of manual pitting manners such as injury to hands and high labor intensity.

The invention relates to a method for cryopreservation and resuscitation of mesenchymal stem cells. A method for preparation and cryopreservation of the mesenchymal stem cells comprises the followingsteps of disinfection and cleaning, digestion treatment, cell culture, cell passage, addition of a cell cryopreservation solution to the mesenchymal stem cells and cryopreservation in liquid nitrogen.The cell cryopreservation solution contains DMEM-F12, dimethyl sulfoxide, canine blood albumin and the like. The method for cryopreservation and resuscitation of the mesenchymal stem cells comprisesthe following steps that the prepared mesenchymal stem cells and the cell cryopreservation solution are uniformly mixed, then the cell solution is placed in the liquid nitrogen for freezing, and cryopreservation is conducted until the cells are needed; when the cells need to be resuscitated, the cryopreserved cells are taken out of the liquid nitrogen and quickly put into a water bath of 37 DEG Cto make the cryopreservation solution completely thawed, and after the cells are thawed, the cells are immediately transferred to an ice bath to complete the resuscitation of the cells. The inventionfurther relates to the cell cryopreservation solution for the cryopreservation and resuscitation of the mesenchymal stem cells. The method and the cryopreservation solution have excellent technical advantages shown in the description.

The invention relates to a method for improving the survival of mesenchymal stem cells after resuscitation and an adopted cryopreservation solution. Particularly, the invention relates to the method for cryopreservation and resuscitation of the mesenchymal stem cells. The method comprises the following steps that the prepared mesenchymal stem cells are uniformly mixed with the cell cryopreservation solution, and then the cell solution is placed in liquid nitrogen for freezing until the cells are needed; when the cells need to be resuscitated, the cryopreserved cells are taken out of the liquidnitrogen and quickly put into a water bath of 37 DEG C to make the cryopreservation solution completely thawed, and after the cells are thawed, the cells are immediately transferred to an ice bath of4 DEG C to complete the resuscitation of the cells. The invention further relates to the cell cryopreservation solution for the cryopreservation and resuscitation of the mesenchymal stem cells. The cell cryopreservation solution contains DMEM-F12, dimethyl sulfoxide, canine blood albumin and the like. The invention further relates to a method for preparation of the mesenchymal stem cells from thecanine umbilical cord and cryopreservation of the mesenchymal stem cells. The methods and the cryopreservation solution have excellent technical advantages shown in the description.

The invention discloses a preparation method of a full porous silica microparticle chiral chromatography packing. The method comprises the following steps: uniformly mixing tetraethoxysilane, absolute ethyl alcohol, a stabilizer and a pore-foaming agent, and then rising the temperature under the protection of inert gas to perform reaction, so as to obtain polyethoxysiloxane; uniformly mixing the polyethoxysiloxane, deionized water and absolute ethyl alcohol, and then sequentially performing gel coagulation, ageing, washing, drying and heat treatment, so as to obtain porous gel microparticles; packing the gel microparticles into 0.1g/ml hydrochloric acid, so as to be subjected to backflow activization, then putting the gel microparticles into a 0.1g/ml amylase-tris(3,5-dimethylphenylcarbamate) solution, stirring the gel microparticles for 5 to 7 hours, performing modification treatment on the gel microparticles, and finally performing drying, so as to obtain the full porous silica microparticle chiral chromatography packing.

The invention relates to mesenchymal stem cells from canine fetal membranes, a preparation method and a used culture medium. The mesenchymal stem cells from the canine fetal membranes are prepared bythe method including the following steps of: disinfection and cleaning, digestion treatment, cell culture, cell passage and cell cryopreservation. The method for preparing the mesenchymal stem cells from the canine fetal membranes shows excellent technical effects, and by the used culture medium, the efficiency of preparation of the stem cells can be greatly improved. The prepared mesenchymal stemcells has the beneficial effect of treating arthritis, bone fracture, muscle injury, ligamentous injury, cartilage injury, joint injury, cognitive disorder, immunologically mediated diseases, xerophthalmia, recurrent uveitis, liver diseases, heart disease, kidney disease, diabetes, gastrointestinal disease, thyroid disease and skin disease of canine animals.

The invention relates to a method for preparing a canine fetal membrane mesenchymal stem cell and the canine fetal membrane mesenchymal stem cell, in particular to a cell freezing medium for the canine fetal membrane mesenchymal stem cell. The method for preparing the canine fetal membrane mesenchymal stem cell comprises the following steps: sterilizing and cleaning, digestive treatment, cell culture, cell passage and cell freezing. The method for preparing the canine fetal membrane mesenchymal stem cell, disclosed by the invention, shows the following excellent technical benefits; in addition, the used culture medium can greatly improve the preparation efficiency of stem cells. The prepared mesenchymal stem cell can beneficially treat arthritis, fracture, muscle injury, ligamentous injury, cartilage injury, joint injury, cognitive impairment, immunologically mediated disease, xerophthalmia, recurrent uveitis, liver diseases, heart disease, kidney disease, diabetes, gastrointestinal disease, thyroid disease and skin disease of canine.

The present invention discloses a kind of magnetic attraction device whose attraction is reliable and attraction elimination is convenient. It is characterized by that it includes a permanent magnet, between the permanent magnet and attracted magnetic conductive metal an intermediate body which is formed from at least two magnetic relay batons and a magnetic short-circuit bridge is mounted, and said intermediate body can be moved relatively to the permanent magnet.

The invention relates to a compound ion fluorinated alkyl sodium sulfate polymer degradation agent and a preparation method and application. The compound ion fluorinated alkyl sodium sulfate polymer degradation agent comprises the following components in percentage by weight: 0.3-0.5% of perfluorophosphate, 1-1.5% of alkyl benzene sulfonic acid, 20-25% of critic acid, 5-10% of ethylene glycol, 15-20% of ferrous sulfate and the balance of water. The compound ion fluorinated alkyl sodium sulfate polymer degradation agent provided by the invention is suitable for reducing viscosity and assisting filtration of a polymer-containing produced liquid in oil field production, and is wide in applicable temperature range, and the crude oil and the polymer-containing produced liquid are successfully separated at a temperature over the freezing point of the crude oil of the produced liquid. Through multiple experiences, the oil content in filtered water is less than 0.02% which reaches the national standard, and the compound ion fluorinated alkyl sodium sulfate polymer degradation agent is energy-saving and environmental-friendly, the pollution is reduced, and the ecological environment is extremely improved.

The invention discloses a fibrous-protein-modified open-tubular column and an application of the fibrous-protein-modified open-tubular column to monoclonal antibody isomer separation. A preparing method of the open-tubular column includes the following steps that fibrous protein is attached to the inner surface of a capillary column in a physical mode, and the fibrous-protein-modified open-tubular column is obtained. According to the open-tubular column, protein serves as a chromatographic stationary phase, attachment of the protein can be effectively inhibited through rich acting sites on the surface of the open-tubular column, the separation effect is improved, and the separation selectivity is improved. The fibrous-protein-modified open-tubular column has the specific monoclonal-antibody-isomer selectivity; six-different-form isomers of cetuximab are successfully separated, and five isomers of rituximab and five isomers of trastuzumab are successfully separated out of a main peak.

The invention relates to a method for alleviating or improving vascular diseases by using a cell therapeutic agent of CD34 positive cells. On the one hand, the related cell therapy composition comprises CD34+ cells and a pharmaceutically-acceptable carrier, for example, the composition comprises CD34+ cells, sodium chloride and water for injection, wherein the density of the CD34+ cells is 1*106 cells/ml to 5*106 cells/ml, and the mass volume percentage of the sodium chloride is 0.8% to 1.0% or 0.9%. The invention further relates to a preparation method of the cell therapeutic agent containingCD34 positive cells and an application of the cell therapeutic composition in preparing drugs for relieving or improving vascular diseases, wherein the vascular diseases are peripheral arterial diseases caused by limb arterial stenosis and occlusion or diabetes, preferably lower limb arterial diseases caused by limb arterial stenosis and occlusion or diabetes. The method shows an excellent technical effect.