49results about How to "Successful separation" patented technology

The invention relates to a method for separating and amplifying a mesenchymal stem cell from a fresh tissue of an umbilical cord. The method comprises the following steps of: sterilizing and cleaning an umbilical cord tissue; shearing the tissue into a block shape; carrying out digestion treatment for 1-2.5 hours and ending the digestion; cleaning the cell obtained by digesting to obtain a cell suspension; adding the cell suspension into a T25 cell culture bottle for culturing; supplementing after culturing the cell suspension for 3-6 days and continuously culturing; carrying out full liquid change for the first time when the cell suspension is cultured for 8-10 days and then carrying out one full medium change every one to three days; when the fusion rate of anchorage-dependent cells in a plate reaches about 50-70 percent, enabling the anchorage-dependent cells to be separated from the bottom of the T25 cell culture bottle by using digestive enzyme; removing supernate by centrifuging, adding a mesenchymal stem cell culture medium for re-suspending the cell, inoculating the cell to the T25 cell culture bottle for carrying out passage and amplifying culture; and then changing liquid once every 1-3 days until the fusion rate reaches 70-90 percent and obtaining the umbilical cord mesenchymal stem cell. The method disclosed by the invention can be effectively used for separating and augmenting the mesenchymal stem cell.

The invention, which belongs to the vacuum electronic technology field, relates to an input / output structure of a broadband phase shift travelling wave tube. The structure comprises six parts of waveguide type elements: a first double-ridge loading rectangular wave guide; a double-ridge loading rectangular wave guide, which is in a bending state of 90 degrees; a second double-ridge loading rectangular wave guide; a double-ridge gradient double-ridge loading rectangular wave guide; a first rectangular wave guide and a second rectangular wave guide. The first double-ridge loading rectangular wave guide and the second double-ridge loading rectangular wave guide are respectively connected with two ends of the double-ridge loading rectangular wave guide that is in a 90 degree bending; the double-ridge gradient double-ridge loading rectangular wave guide is connected between the second double-ridge loading rectangular wave guide and the first rectangular wave guide; and the second rectangular wave guide is connected with an outboard curved surface window of the double-ridge loading rectangular wave guide that is in a 90 degree bending, wherein a central axis of the double-ridge loading rectangular wave guide that is in a 90 degree bending is superposed with a central axis of the first rectangular wave guide. According to the invention, broadband microwave signal energy and a band shape electronic beam can be well introduced and a sine wave guide structure can be lead out; besides, the input / output structure is simple and is easy to process and realize.

The invention provides a bag breaking mechanism and an automatic object and bag separation system. A garbage bag is grabbed to the position above the bag breaking mechanism through a movable mechanical arm, then the plastic garbage bag is scratched through the bag breaking mechanism, and reliable separation of garbage and the garbage bag is achieved. The automatic object and bag separation systemis characterized by comprising a mechanical arm unit, a horizontal moving unit, the bag breaking mechanism, a power unit and a control unit, the mechanical arm unit is used for grabbing the bag put into the bag breaking mechanism, the horizontal moving unit is used for driving the mechanical arm unit to horizontally move above the bag breaking mechanism, the bag breaking mechanism is used for tearing the bag after the mechanical arm unit grabs the bag, so that the object in the bag falls off, and the bag is separated from the object in the bag, the power unit provides power for the mechanicalarm unit, the horizontal moving unit and the bag breaking mechanism, and the control unit controls the mechanical arm unit, the horizontal moving unit, the bag breaking mechanism and the power unit tooperate.

The invention relates to a method for preparing CD34 positive cells from umbilical cord mesenchymal stem cells. The method specifically comprises the following steps: (a) providing mesenchymal stem cells from an umbilical cord; (b) performing primary culture on the mesenchymal stem cells; (c) performing subculture on the mesenchymal stem cells; (d) in a subculture process, adding an inductive agent in a culture medium for inducing the mesenchymal stem cells; (e) obtaining the CD34 positive cells. The invention also relates to cell products of the CD34 positive cells, prepared through the method and application of the cell products. The method disclosed by the invention has excellent technical effects described in the description.

The invention relates to canine umbilical cord mesenchymal stem cells as well as a preparation method and a cryopreservation method thereof. Specifically, the method comprises the following steps: disinfection and washing, digestion treatment, cell culture, cell passage, and cell cryopreservation. The method for preparing mesenchymal stem cells from a canine umbilical cord exhibits an excellent technical effect. The mesenchymal stem cells is beneficial for treatment of canine arthritis, fractures, muscle damage, ligament injury, cartilage damage, joint damage, cognitive dysfunction, immune-mediated diseases, dry eye, recurrence uveitis, liver disease, heart disease, kidney disease, diabetes, gastrointestinal disease, thyroid disease and skin disease.

Provided is a method for separating and extracting mesenchymal stem cells from the human umbilical cord. The method uses healthy neonatal umbilical cord tissue; after cleaning and disinfection, mechanically pulverising same, separating the Wharton's jelly, and after treating with erythrocyte lysate, carrying out suspension culture in a serum-free culture medium. Replacing the liquid every 3-5 days; after the plate adherence rate reaches 30-70%, carrying out trypsin digestion, and then collecting the cells by centrifugation for passage amplification, until the rate of confluence of the cells reaches 80-90% confluence, thereby obtaining high purity umbilical cord mesenchymal stem cells.

The invention relates to a method for preparing mesenchymal stem cells from the umbilical cord of a dog. Specifically, the method comprises the following steps: sterilization and cleaning, digestion treatment, cell culture, cell passage and cell cryopreservation. The method for preparing mesenchymal stem cells from the umbilical cord of the dog, which is provided by the invention, has excellent technical effects; the obtained mesenchymal stem cells are beneficial to treatment on arthritis, bone fracture, muscle injury, ligamentous injury, cartilage injury, joint injury, cognition impairment, immune-mediated diseases, xerophthalmia, recurrent uveitis, hepatic diseases, heart diseases, kidney diseases, diabetes, gastrointestinal diseases, thyroid diseases and skin diseases of canidae.

The invention relates to a coin processing device, in particular to a coin processing device capable of individually and reliably separating a great number of disorderedly inserted coins and identifying the coins. The coin processing device comprises a coin slot, a rotation disc for separating the coins and a coin identification module, the coin separating rotation disc is rotationally arranged on an oblique rotation disc base, a guard plate for preventing the coins from falling off the base is arranged on the periphery of the rotation disc, at least one coin falling groove with the size slightly larger than that of each coin is arranged on the rotation disc, a coin outlet passing by the coin falling grooves during rotating is arranged on the rotation disc base, the guard plate comprises a small-diameter ring and a large-diameter ring, the inner diameter of the large-diameter ring is slightly larger than the diameter of the rotation disc, the large-diameter ring and the rotation disc base are fixedly matched to enable the rotation disc to be disposed inside the large-diameter ring and away from the small-diameter ring, and the inner oblique surface of the large-diameter ring at the lowest section forms an inverted-conical gradient curved surface to be in transition connection with the inner surface of the small-diameter ring.

The invention discloses a synthetic method of 2,3-dichloropyridine(III). The synthetic method of 2,3-dichloropyridine comprises the following steps: (1) taking pyridine (I) as a main starting raw material, taking water or water vapor as a reaction medium, reacting the pyridine with chlorine completely in the presence of a chlorination inducer to obtain pyridine chloride (II) as a reaction raw material for the next step; (2) taking the pyridine chloride (II) obtained from the first step as the reaction raw material, taking water or water vapor as a reaction medium, enabling the pyridine chloride (II) to be in contact with hydrogen in the presence of an acid-bining agent and a catalyst, and carrying out catalyzed hydrogeneration reduction reaction to obtain the 2,3-dichloropyridine (III) of which the purity is 99% or above, wherein the catalyst is a nano IrO2-ZnO-MnO2 composite catalyst. A reaction equation is as shown in the specification, wherein n ranges from 2 to 5. The synthetic method of 2,3-dichloropyridine has the advantages of high reaction conversion, high selectivity, stable product quality, simplicity in production and operation, low cost and the like, and the shortcomings that the quality of the 2,3-dichloropyridine (III) obtained by an existing synthetic method is not stable, and the quality of the 2,3-dichloropyridine (III) can reach up to the standard after the 2,3-dichloropyridine (III) is rectified repeatedly necessarily are overcome.

The invention discloses a method for separating and recycling vanadium and chromium from chromium vanadium reducing slag. The method comprises the following steps that (1) the chromium vanadium reducing slag and oxidability chromium salt are roasted, wherein the roasting condition comprises the temperature of 300 DEG C to 400 DEG C and the time of 60 minutes to 240 minutes; (2) water leaching is carried out on roasted materials in the step (1), then, solid-liquid separation is carried out on the materials, and lixivium containing the vanadium is obtained; (3) acid leaching and / or alkaline leaching are / is carried out on a solid phase obtained after solid-liquid separation in the step (2), then solid-liquid separation is carried out on the solid phase, and lixivium containing the chromium is obtained. Through the method, the vanadium in the chromium vanadium reducing slag can be directly oxidized at the low roasting temperature, the vanadium can be extracted through following water leaching, the chromium is extracted through following acid leaching and / or alkaline leaching, and the vanadium and the chromium in the chromium vanadium reducing slag can be successfully separated.

The invention discloses a freestone type peach pitting device. The freestone type peach pitting device comprises a device support, a transmission mechanism, a valve opening mechanism and a control circuit board, wherein the transmission mechanism comprises a driving motor, a driving chain wheel and a driven chain well, wherein the driving chain wheel and the driven chain wheel are correspondingly arranged on the upper portion of the device support and provided with two transmission chains in a sleeved mode; the driving motor is arranged under the device support and connected with the driving chain wheel in a chain transmission mode; the valve opening mechanism comprises a pressing device, a stone valve separating mechanism, a detecting and positioning unit and at least one group of initial positioning unit. By means of the initial positioning unit, the pressing device and the stone valve separating mechanism, the freestone type peach pitting device can easily achieve separation of peach stones and pulp, thereby effectively solving the problems of manual pitting manners such as injury to hands and high labor intensity.

The invention relates to an algae setting and purifying platform, which is characterized by setting a floating platform, which has a collection area, a reaction area, a concentration area and an isolation area in turn from the front end to the back end, wherein, manual operation areas are respectively arranged on two sides of the reaction area, the concentration area and the isolation area; the floating platform is arranged on two side edges of watertight isolating materials; an upper edge of the two side edges is provided with a floating body of air cell type; two side edges of the collection area are in the shape of a speaker and are toward a front opening of the platform; a hollow bottom is arranged between the two side edges of the collection area; bottoms of the reaction area, the concentration area and the isolation area between the two side edges form a sieve layer. The algae setting and purifying platform can carry out urgent isolation and remove the burst cyanobacteria in water of a large area with a high-efficiency, thus decreasing the environment danger due to the burst of the cyanobacteria and avoiding secondary pollution.

The invention relates to a method for cryopreservation and resuscitation of mesenchymal stem cells. A method for preparation and cryopreservation of the mesenchymal stem cells comprises the followingsteps of disinfection and cleaning, digestion treatment, cell culture, cell passage, addition of a cell cryopreservation solution to the mesenchymal stem cells and cryopreservation in liquid nitrogen.The cell cryopreservation solution contains DMEM-F12, dimethyl sulfoxide, canine blood albumin and the like. The method for cryopreservation and resuscitation of the mesenchymal stem cells comprisesthe following steps that the prepared mesenchymal stem cells and the cell cryopreservation solution are uniformly mixed, then the cell solution is placed in the liquid nitrogen for freezing, and cryopreservation is conducted until the cells are needed; when the cells need to be resuscitated, the cryopreserved cells are taken out of the liquid nitrogen and quickly put into a water bath of 37 DEG Cto make the cryopreservation solution completely thawed, and after the cells are thawed, the cells are immediately transferred to an ice bath to complete the resuscitation of the cells. The inventionfurther relates to the cell cryopreservation solution for the cryopreservation and resuscitation of the mesenchymal stem cells. The method and the cryopreservation solution have excellent technical advantages shown in the description.

The invention relates to a method for improving the survival of mesenchymal stem cells after resuscitation and an adopted cryopreservation solution. Particularly, the invention relates to the method for cryopreservation and resuscitation of the mesenchymal stem cells. The method comprises the following steps that the prepared mesenchymal stem cells are uniformly mixed with the cell cryopreservation solution, and then the cell solution is placed in liquid nitrogen for freezing until the cells are needed; when the cells need to be resuscitated, the cryopreserved cells are taken out of the liquidnitrogen and quickly put into a water bath of 37 DEG C to make the cryopreservation solution completely thawed, and after the cells are thawed, the cells are immediately transferred to an ice bath of4 DEG C to complete the resuscitation of the cells. The invention further relates to the cell cryopreservation solution for the cryopreservation and resuscitation of the mesenchymal stem cells. The cell cryopreservation solution contains DMEM-F12, dimethyl sulfoxide, canine blood albumin and the like. The invention further relates to a method for preparation of the mesenchymal stem cells from thecanine umbilical cord and cryopreservation of the mesenchymal stem cells. The methods and the cryopreservation solution have excellent technical advantages shown in the description.

The invention discloses a preparation method of a full porous silica microparticle chiral chromatography packing. The method comprises the following steps: uniformly mixing tetraethoxysilane, absolute ethyl alcohol, a stabilizer and a pore-foaming agent, and then rising the temperature under the protection of inert gas to perform reaction, so as to obtain polyethoxysiloxane; uniformly mixing the polyethoxysiloxane, deionized water and absolute ethyl alcohol, and then sequentially performing gel coagulation, ageing, washing, drying and heat treatment, so as to obtain porous gel microparticles; packing the gel microparticles into 0.1g / ml hydrochloric acid, so as to be subjected to backflow activization, then putting the gel microparticles into a 0.1g / ml amylase-tris(3,5-dimethylphenylcarbamate) solution, stirring the gel microparticles for 5 to 7 hours, performing modification treatment on the gel microparticles, and finally performing drying, so as to obtain the full porous silica microparticle chiral chromatography packing.

The invention relates to mesenchymal stem cells from canine fetal membranes, a preparation method and a used culture medium. The mesenchymal stem cells from the canine fetal membranes are prepared bythe method including the following steps of: disinfection and cleaning, digestion treatment, cell culture, cell passage and cell cryopreservation. The method for preparing the mesenchymal stem cells from the canine fetal membranes shows excellent technical effects, and by the used culture medium, the efficiency of preparation of the stem cells can be greatly improved. The prepared mesenchymal stemcells has the beneficial effect of treating arthritis, bone fracture, muscle injury, ligamentous injury, cartilage injury, joint injury, cognitive disorder, immunologically mediated diseases, xerophthalmia, recurrent uveitis, liver diseases, heart disease, kidney disease, diabetes, gastrointestinal disease, thyroid disease and skin disease of canine animals.

The invention relates to a method for preparing a canine fetal membrane mesenchymal stem cell and the canine fetal membrane mesenchymal stem cell, in particular to a cell freezing medium for the canine fetal membrane mesenchymal stem cell. The method for preparing the canine fetal membrane mesenchymal stem cell comprises the following steps: sterilizing and cleaning, digestive treatment, cell culture, cell passage and cell freezing. The method for preparing the canine fetal membrane mesenchymal stem cell, disclosed by the invention, shows the following excellent technical benefits; in addition, the used culture medium can greatly improve the preparation efficiency of stem cells. The prepared mesenchymal stem cell can beneficially treat arthritis, fracture, muscle injury, ligamentous injury, cartilage injury, joint injury, cognitive impairment, immunologically mediated disease, xerophthalmia, recurrent uveitis, liver diseases, heart disease, kidney disease, diabetes, gastrointestinal disease, thyroid disease and skin disease of canine.

The present invention discloses a kind of magnetic attraction device whose attraction is reliable and attraction elimination is convenient. It is characterized by that it includes a permanent magnet, between the permanent magnet and attracted magnetic conductive metal an intermediate body which is formed from at least two magnetic relay batons and a magnetic short-circuit bridge is mounted, and said intermediate body can be moved relatively to the permanent magnet.

The invention discloses a modified aluminium oxide microsphere, and a preparation method and application thereof. The modified aluminium oxide microsphere possesses the specific surface area of 207.88 m<2> / g, and the adsorption accumulated total pore volume or desorption accumulated total pore volume of 0.51 cm<3> / g. The preparation method comprises successively adding tween-80, span-80 and heptan-1-ol into n-heptane under the conditions of stirring and heating, then adding an aluminium nitrate solution, stirring at a constant temperature of 50-70 DEG C for a period, adding ammonia water with the concentration of 1 mol / L drop by drop, continuing to stir at a constant temperature of 50-70 DEG C for 1-3 h, cooling and filtering to obtain a colloidal precipitate, and performing cleaning, drying, calcination, cooling and oscillation, and then performing filtering and drying, so as to obtain the modified aluminium oxide microsphere. Compared with an aluminium oxide microsphere prepared through a traditional method, the aluminium oxide microsphere prepared according to the disclosed preparation method possesses the specific surface area larger by about 18%, and possesses the total pore volume larger by about 25%, and is successfully applicable to separation of uranium, molybdenum and neodymium in spent fuel elements.

The invention relates to a compound ion fluorinated alkyl sodium sulfate polymer degradation agent and a preparation method and application. The compound ion fluorinated alkyl sodium sulfate polymer degradation agent comprises the following components in percentage by weight: 0.3-0.5% of perfluorophosphate, 1-1.5% of alkyl benzene sulfonic acid, 20-25% of critic acid, 5-10% of ethylene glycol, 15-20% of ferrous sulfate and the balance of water. The compound ion fluorinated alkyl sodium sulfate polymer degradation agent provided by the invention is suitable for reducing viscosity and assisting filtration of a polymer-containing produced liquid in oil field production, and is wide in applicable temperature range, and the crude oil and the polymer-containing produced liquid are successfully separated at a temperature over the freezing point of the crude oil of the produced liquid. Through multiple experiences, the oil content in filtered water is less than 0.02% which reaches the national standard, and the compound ion fluorinated alkyl sodium sulfate polymer degradation agent is energy-saving and environmental-friendly, the pollution is reduced, and the ecological environment is extremely improved.

The invention discloses a synthetic method of 2,3-dichloropyridine(III). The synthetic method of 2,3-dichloropyridine comprises the following steps: (1) taking pyridine (I) as a main starting raw material, taking water or water vapor as a reaction medium, reacting the pyridine with chlorine completely in the presence of a chlorination inducer to obtain pyridine chloride (II) as a reaction raw material for the next step; (2) taking the pyridine chloride (II) obtained from the first step as the reaction raw material, taking water or water vapor as a reaction medium, enabling the pyridine chloride (II) to be in contact with hydrogen in the presence of an acid-bining agent and a catalyst, and carrying out catalyzed hydrogeneration reduction reaction to obtain the 2,3-dichloropyridine (III) of which the purity is 99% or above, wherein the catalyst is a nano IrO2-ZnO-MnO2 composite catalyst. A reaction equation is as shown in the specification, wherein n ranges from 2 to 5. The synthetic method of 2,3-dichloropyridine has the advantages of high reaction conversion, high selectivity, stable product quality, simplicity in production and operation, low cost and the like, and the shortcomings that the quality of the 2,3-dichloropyridine (III) obtained by an existing synthetic method is not stable, and the quality of the 2,3-dichloropyridine (III) can reach up to the standard after the 2,3-dichloropyridine (III) is rectified repeatedly necessarily are overcome.

The invention discloses a device and a method for low-temperature variable-pressure absorption of chloromethane in waste gas by ionic liquid, and belongs to the technical field of fine chemical separation and purification. An ionic liquid absorbent is adopted, through a low-temperature pressure swing absorption method, chloromethane-containing waste gas enters a gas absorption tower (T1) from the tower bottom, the absorbent enters the gas absorption tower (T1) from the tower top, the bottom material flow of the gas absorption tower (T1) is connected with the middle part of a first flash tank (S1), and air containing trace chloromethane is emptied from the top of the first flash tank (S1); an absorbent rich in chloromethane gas enters the middle of the second flash tank (S2) from the bottom of the first flash tank (S1), the product chloromethane gas is extracted from the top of the second flash tank (S2), and the absorbent is extracted from the bottom of the second flash tank (S2), subjected to heat exchange through the heat exchanger (H1) and then conveyed back to the top of the gas absorption tower (T1) through the conveying pump (P1). The method has the characteristics of low energy consumption, short flow, low equipment cost, high absorption capacity, recyclable absorbent and the like.

The invention discloses a fibrous-protein-modified open-tubular column and an application of the fibrous-protein-modified open-tubular column to monoclonal antibody isomer separation. A preparing method of the open-tubular column includes the following steps that fibrous protein is attached to the inner surface of a capillary column in a physical mode, and the fibrous-protein-modified open-tubular column is obtained. According to the open-tubular column, protein serves as a chromatographic stationary phase, attachment of the protein can be effectively inhibited through rich acting sites on the surface of the open-tubular column, the separation effect is improved, and the separation selectivity is improved. The fibrous-protein-modified open-tubular column has the specific monoclonal-antibody-isomer selectivity; six-different-form isomers of cetuximab are successfully separated, and five isomers of rituximab and five isomers of trastuzumab are successfully separated out of a main peak.

The invention discloses a fibrous-protein-modified open-tubular column and an application of the fibrous-protein-modified open-tubular column to monoclonal antibody isomer separation. A preparing method of the open-tubular column includes the following steps that fibrous protein is attached to the inner surface of a capillary column in a physical mode, and the fibrous-protein-modified open-tubular column is obtained. According to the open-tubular column, protein serves as a chromatographic stationary phase, attachment of the protein can be effectively inhibited through rich acting sites on the surface of the open-tubular column, the separation effect is improved, and the separation selectivity is improved. The fibrous-protein-modified open-tubular column has the specific monoclonal-antibody-isomer selectivity; six-different-form isomers of cetuximab are successfully separated, and five isomers of rituximab and five isomers of trastuzumab are successfully separated out of a main peak.

The invention, which belongs to the vacuum electronic technology field, relates to an input / output structure of a broadband phase shift travelling wave tube. The structure comprises six parts of waveguide type elements: a first double-ridge loading rectangular wave guide; a double-ridge loading rectangular wave guide, which is in a bending state of 90 degrees; a second double-ridge loading rectangular wave guide; a double-ridge gradient double-ridge loading rectangular wave guide; a first rectangular wave guide and a second rectangular wave guide. The first double-ridge loading rectangular wave guide and the second double-ridge loading rectangular wave guide are respectively connected with two ends of the double-ridge loading rectangular wave guide that is in a 90 degree bending; the double-ridge gradient double-ridge loading rectangular wave guide is connected between the second double-ridge loading rectangular wave guide and the first rectangular wave guide; and the second rectangular wave guide is connected with an outboard curved surface window of the double-ridge loading rectangular wave guide that is in a 90 degree bending, wherein a central axis of the double-ridge loading rectangular wave guide that is in a 90 degree bending is superposed with a central axis of the first rectangular wave guide. According to the invention, broadband microwave signal energy and a band shape electronic beam can be well introduced and a sine wave guide structure can be lead out; besides, the input / output structure is simple and is easy to process and realize.

The invention relates to a method for preparing CD34 positive cells from umbilical cord mesenchymal stem cells. The method specifically comprises the following steps: (a) providing mesenchymal stem cells from an umbilical cord; (b) performing primary culture on the mesenchymal stem cells; (c) performing subculture on the mesenchymal stem cells; (d) in a subculture process, adding an inductive agent in a culture medium for inducing the mesenchymal stem cells; (e) obtaining the CD34 positive cells. The invention also relates to cell products of the CD34 positive cells, prepared through the method and application of the cell products. The method disclosed by the invention has excellent technical effects described in the description.

The invention relates to a method for alleviating or improving vascular diseases by using a cell therapeutic agent of CD34 positive cells. On the one hand, the related cell therapy composition comprises CD34+ cells and a pharmaceutically-acceptable carrier, for example, the composition comprises CD34+ cells, sodium chloride and water for injection, wherein the density of the CD34+ cells is 1*106 cells / ml to 5*106 cells / ml, and the mass volume percentage of the sodium chloride is 0.8% to 1.0% or 0.9%. The invention further relates to a preparation method of the cell therapeutic agent containingCD34 positive cells and an application of the cell therapeutic composition in preparing drugs for relieving or improving vascular diseases, wherein the vascular diseases are peripheral arterial diseases caused by limb arterial stenosis and occlusion or diabetes, preferably lower limb arterial diseases caused by limb arterial stenosis and occlusion or diabetes. The method shows an excellent technical effect.

The invention belongs to the field of medicine separation and analysis, and particularly relates to a cis-isomer of anisodamine and a separation and detection method of the cis-isomer. Through combination of HPLC and supercritical chromatography, the cis-isomer of racanisodamine can be effectively separated, and then through creative chromatographic parameter selection in HPLC and supercritical chromatography, the separation degree is improved, so that the purity of the two finally obtained cis-isomer monomers of anisodamine is high, and the purity of the two obtained cis-isomer monomers is high. And a foundation is laid for large-scale industrial application.

The invention discloses an artificial cultivation method of Wuling ginseng. The artificial cultivation method of Wuling ginseng comprises the steps of preparing strains of Wuling ginseng, preparing liquid strains of Wuling ginseng, and cultivating Wuling ginseng, specifically comprising: Preparation of ginseng strains: take fresh Wuling ginseng, sterilize, cut off the internal tissue and insert it into the solid medium for strain separation to culture the tissue for germination, purify 1~3 times of gene detection, and then obtain the target Wuling ginseng strain; Preparation of Wulingshen liquid strain: inoculate the Wulingshen strain into a liquid medium for shaking culture to obtain the target Wulingshen liquid strain; Wulingshen cultivation steps: liquid inoculate the Wulingshen liquid strain, The Wuling ginseng is cultivated until it is mature, and then picked; the present invention successfully domesticates and cultivates the Wuling ginseng, and has the advantages of simple method and sufficient raw materials, so it can be used for further research and exert its greater value. Several sets of experiments have proved that the medium cultivation method of the present invention can successfully domesticate Wuling ginseng.