A kind of artificial cultivation method of Wuling ginseng

A technology of artificial cultivation and Uling ginseng, which is applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problems of high cost and the destruction price of wild resources, and achieve the effects of low cost, simple cultivation method and easy mastery.

Active Publication Date: 2022-01-11
KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] In recent years, there have been many studies on C. nigriscens and its sclerotia-Wulingshen, an important medicinal fungus, many people know that it has high medicinal value, but because Wulingshen cannot be cultivated artificially at present Serious damage to wild resources and high prices
At present, there is no artificial cultivation report of Wuling ginseng, and this invention is the first time that Wuling ginseng has been artificially cultivated

Method used

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Examples

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Effect test

preparation example Construction

[0027] The preparation method of described strain isolation medium comprises the following steps:

[0028] 1) Cut the peeled potatoes and fresh Wuling ginseng into small pieces and mix well to obtain material a, add water to material a, boil and filter to obtain filtrate b;

[0029] 2) Add glucose, agar powder, peptone, potassium dihydrogen sulfate, magnesium sulfate and vitamin B to the filtrate b, add water to the volume, stir to dissolve, subpackage, sterilize and pour the plate to obtain the target strain isolation medium .

[0030] Its specific operation is as follows:

[0031] Step 1: Cut 200 g of peeled potatoes into small pieces, 20 g of fresh Wuling ginseng, cut into small pieces, add 500ml of water and boil until rotten;

[0032] Step 2: Filter, take the filtrate, add 20g of glucose, 20g of agar powder, 5g of peptone, 2g of potassium dihydrogen phosphate, 2g of magnesium sulfate, and 1-3 tablets of vitamin B;

[0033] Step 3: Add water to make up to 1000 mL;

[0...

Embodiment 1

[0068] The formula is: 60% sawdust, 24% wheat bran, 14% wheat, 1% gypsum, 0.5% potassium dihydrogen phosphate, and 0.5% magnesium sulfate as cultivation materials. The domestication and cultivation of Wuling ginseng includes the following steps:

[0069] (1) Mix the ingredients according to the formula, and the water content is 60%;

[0070] (2) Use 650mL tissue culture bottle as the cultivation container;

[0071] (3) Compact the filling of each bottle to one-third;

[0072] (4) Sterilization, using high-pressure steam at a temperature of 121°C for 2h to 3h;

[0073] (5) Take out and cool after the sterilization is completed;

[0074] (6) Cooling and inoculation, when the temperature of the cultivation medium is cooled to about 25°C, liquid inoculation is carried out, and the liquid strain of Wuling ginseng prepared in advance is used, and the inoculation amount is 5% to 8% of the mass of the culture medium;

[0075] (7) Mycelia culture, the temperature is controlled at 24...

Embodiment 2

[0080] The formula is: 40% cottonseed husk, 20% sawdust, 14% wheat, 1% gypsum, 0.5% potassium dihydrogen phosphate, and 0.5% magnesium sulfate as cultivation materials. The domestication and cultivation of Wuling ginseng includes the following steps:

[0081] (1) Mix the ingredients according to the formula, and the water content is 60%;

[0082] (2) Use 650mL tissue culture bottle as the cultivation container;

[0083] (3) Compact the filling of each bottle to one-third;

[0084] (4) Sterilization, using high-pressure steam at a temperature of 121°C for 2h to 3h;

[0085] (5) Take out and cool after the sterilization is completed;

[0086] (6) Cooling and inoculation, when the temperature of the cultivation medium is cooled to about 25°C, liquid inoculation is carried out, and the liquid strain of Wuling ginseng prepared in advance is used, and the inoculation amount is 5% to 8% of the mass of the culture medium;

[0087] (7) Mycelia culture, the temperature is controlled ...

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Abstract

The invention discloses an artificial cultivation method of Wuling ginseng. The artificial cultivation method of Wuling ginseng comprises the steps of preparing strains of Wuling ginseng, preparing liquid strains of Wuling ginseng, and cultivating Wuling ginseng, specifically comprising: Preparation of ginseng strains: take fresh Wuling ginseng, sterilize, cut off the internal tissue and insert it into the solid medium for strain separation to culture the tissue for germination, purify 1~3 times of gene detection, and then obtain the target Wuling ginseng strain; Preparation of Wulingshen liquid strain: inoculate the Wulingshen strain into a liquid medium for shaking culture to obtain the target Wulingshen liquid strain; Wulingshen cultivation steps: liquid inoculate the Wulingshen liquid strain, The Wuling ginseng is cultivated until it is mature, and then picked; the present invention successfully domesticates and cultivates the Wuling ginseng, and has the advantages of simple method and sufficient raw materials, so it can be used for further research and exert its greater value. Several sets of experiments have proved that the medium cultivation method of the present invention can successfully domesticate Wuling ginseng.

Description

technical field [0001] The invention belongs to the technical field of cultivation of medicinal fungi, and in particular relates to an artificial cultivation method of Wuling ginseng. Background technique [0002] C. nigricans ( Xylaria nigripes ) belongs to the kingdom of fungi, phylum Ascomycota, subphylum Ascomycota, class, subclass, Xyroceratales, Xyrocerataceae, and Xyrocera genus. C. nigriscens grows in the waste nests of termites, and sclerotia will form in the gap between the soil and termite nests. It is commonly known as Wulingshen and is a valuable traditional Chinese medicine. Wuling ginseng, also known as Wuling bacteria, Lei Zhenzi, chicken poria cocos, chicken fir egg, ground charcoal stick, Wuli ginseng, etc., is spherical, ellipsoidal or ovoid to irregular in shape, occasionally flat, with brown outer skin Black to black, hard, 1-8cm in diameter, one end is connected with the upper fruiting body to form a rhizome, hanging in the termite nest, the other end...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/40A01G18/00A01G18/20
CPCA01G18/40A01G18/00A01G18/20
Inventor 张俊波刘绍雄罗孝坤岳万松李建英马明
Owner KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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