The invention relates to a method for alleviating or improving vascular lesions by a cell therapy agent of CD34 positive cells. In one aspect, the cell therapy compositions involved include CD34 + Cells and pharmaceutically acceptable carriers, for example, containing: CD34 + cells, sodium chloride, water for injection, CD34 + The density of cells is 1×10 6 A / mL~5×10 6 Individual / mL, the mass volume percentage of sodium chloride is 0.8% to 1.0% or 0.9%. It also relates to the preparation method of the cell therapy agent comprising CD34 positive cells, and the use of the cell therapy composition in the preparation of medicines for relieving or improving vascular disease, wherein the vascular disease is caused by stenosis and occlusion of limb arteries or diabetes Peripheral arterial disease; preferred limb arterial stenosis and occlusion or lower extremity arterial disease caused by diabetes. The method of the present invention exhibits excellent technical effects as described in the present invention.