Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

ASD (autism spectrum disorder) detection kit

A kit and autism technology, applied in the field of autism detection kits, can solve problems such as delayed treatment timing and achieve the effect of specific detection

Inactive Publication Date: 2018-02-09
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current routine confirmation methods for autism can only be diagnosed after the patient has symptoms of autism, which delays the timing of treatment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • ASD (autism spectrum disorder) detection kit
  • ASD (autism spectrum disorder) detection kit
  • ASD (autism spectrum disorder) detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: The onset of autism is related to the down-regulation of ERβ gene expression in the offspring’s brain after exposure to prenatal oral contraceptives (COC)

[0033] Three-month-old pregnant SD rats were divided into 4 groups: blank control group (VEH), levonorgestrel group (LNG), ethinyl estradiol group (EE) and levonorgestrel / ethinyl estradiol The alcohol group (LNG / EE) was given subcutaneous injections of 0.1ml saline, 20μg levonorgestrel, 10μg ethinyl estradiol and 20μg levonorgestrel plus 10μg ethinyl estradiol for 21 consecutive days. At the 10th week after the offspring are born, each group randomly selects several male offspring and female offspring for the following experiments:

[0034] 1. Autism behavior study: Use the following widely recognized standard methods to evaluate the autistic behavior of offspring rats, and analyze whether prenatal rats exposed to compound oral contraceptives (COC) lead to autism in offspring Symptomatic behavior.

[0035] (1)...

Embodiment 2

[0051] Example 2: Detection of ERβ and its target gene expression in CD34-positive cells isolated from the bone marrow and peripheral blood of the offspring can be used as biomarkers for the diagnosis of autism

[0052] Three-month-old pregnant SD rats were divided into 4 groups: blank control group (VEH), levonorgestrel group (LNG), ethinyl estradiol group (EE) and levonorgestrel / ethinyl estradiol The alcohol group (LNG / EE) was given subcutaneous injections of 0.1ml saline, 20μg levonorgestrel, 10μg ethinyl estradiol and 20μg levonorgestrel plus 10μg ethinyl estradiol for 21 consecutive days. At the 10th week after the offspring are born, each group randomly selects several male and female offspring, and extracts bone marrow and peripheral blood to separate CD34 positive cells, then extracts mRNA, and uses real-time quantitative PCR to detect ERβ and its targets The gene expression levels of SOD2 and ERRα were analyzed to analyze whether the expression of ERβ and its target gene...

Embodiment 3

[0054] Example 3: A kit for detecting autism

[0055] Including RT reaction solution (RNA reverse transcription reaction), Taq DNA polymerase, PCR amplification detection reagents, primers.

[0056] Among them: the composition of the RT reaction solution is shown in Table 1.

[0057] Table 1 Composition of RT reaction solution

[0058] Component

Dosage

10×Buffer RT

30μl

dNTP Mix(5mM)

20μl

RNase-Free Water

1ml

[0059] PCR amplification detection reagents include: dNTPs, negative control products and positive quality control products.

[0060] The primer is composed of forward primer and reverse primer of one of ERβ, ERα, SOD2, ERRα, OXT, OXTR, GPER, PGR or AR. The length of each primer is about 21 bp, and the sequence is shown in Table 2. Among them, β-actin or GAPDH is used as an internal control.

[0061] Table 2 Primer sequence

[0062]

[0063] Autism gene detection kit also includes TaqMan fluorescent probe and SYBR GREEN I fluorescent dye.

[0064] The autism gene dete...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biology and discloses an ASD (autism spectrum disorder) detection kit. Discoveries find that expression of CD34 positive cell ER beta in bone marrow andperipheral blood and related genes of the CD34 positive cell ER beta is closely related with production of ASD-like behaviors and can serve as markers of ASD for detection and pre-screening of ASD. The ASD detection kit can be used for clinically detecting and pre-screening ASD rapidly, sensitively and specifically, and effective preventing and intervention treatment measures can be taken for children patients with ASD according to detection results.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and specifically relates to a detection kit, especially a kit for autism detection. Background technique [0002] Autism spectrum disorder (ASD, autism for short) is a group of developmental disorders, including complete autism, Asperger's syndrome, and other pervasive developmental disorders. Nowadays, the detection rate of autism is more than 1%, and the prevalence is increasing, and men with autism account for 80%. Children with autism refuse to change their original habits and behaviors, and have difficulty adapting to the new environment. Most of the children also show purposeless activities, hyperactivity, monotonous and repetitive jumping, clapping, waving, running and spinning, and some even appear Self-injury and self-harm, such as repeated nose picking, mouth picking, lip biting, and sucking. The language of children with autism is incompatible with the language of normal people in ter...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
Inventor 陈海佳葛啸虎王一飞陈婉玲姚大纯
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com