Method for separating hematopoietic stem cells from umbilical cord blood and amplifying CD34 positive cells

A technology of hematopoietic stem cells and positive cells, applied in the field of CD34+ cell preparation, can solve the problems of limited supply of human umbilical cord blood, limited number of CD34+ cells, etc.
CN106701682AInactive Publication Date: 2017-05-24BOYALIFE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BOYALIFE
Publication Date
2017-05-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for separating hematopoietic stem cells from umbilical cord blood and amplifying CD34 positive cells. The method comprises the following specific steps: separating hematopoietic stem cells from umbilical cord blood; purifying; separating by using a Ficoll separating solution; counting the CD34 positive cells by using a flow cytometer, diluting, inoculating and culturing; changing the solution and culturing; transferring the cells to new plates and culturing; evaluating the cell amplifying effect. The invention further relates to cell products containing the CD34 positive cells prepared by the method and application of the cell products. The method has excellent technical effects as described in the specification.
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Description

technical field

[0001] The present invention belongs to the field of treating clinical diseases with cell biology techniques. Specifically, the present invention relates to a method for preparing CD34 from umbilical cord blood hematopoietic stem cells + cell method. Background technique

[0002] Stem cells mediate regeneration and the transmission of genetic information to subsequent cell generations. They can self-renew and produce differentiated progeny. In recent years, advances have been made in our understanding of the molecular mechanisms underlying the interactions between stem cells and their tissue microecological environment. This has led to a better understanding of the molecular regulatory mechanisms at work in stem cells.

[0003] While gene therapy is still experimental, the technology holds the promise of having a major impact on human health. The scope and definition of gene therapy has changed and expanded over the past few years. In addition to correc...

Claims

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