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Method for extracting hemopoietic stem cells from placentas

A technology for hematopoietic stem cells and placenta, applied in embryonic cells, germ cells, animal cells, etc., can solve the problems of complex methods, cells susceptible to bacteria, and a small number of hematopoietic stem cells.

Active Publication Date: 2014-11-19
BOYALIFE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Invention Patent Application No. 01131190.8 discloses a method of obtaining hematopoietic stem cells by shredding and digesting. The method is complicated, the number of obtained hematopoietic stem cells is small, the purity is low, and the cells are easily contaminated with bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1: Method for separating and extracting hematopoietic stem cells from placenta

[0073] The placenta came from the obstetrics and gynecology department of the local hospital. First, after obtaining the consent of the pregnant woman or her family to donate the placenta, check all the pregnant women’s examination reports and confirm that there is no virus, syphilis or blood-related viral infection, and then ask the pregnant woman’s family medical history, infection and infection history. After confirming that everything is normal, collect 5 mL of maternal blood, put the placenta and maternal blood into the placenta storage and transportation box, and affix the label and barcode. During the storage and transportation of the placenta, the temperature in the box is maintained at 4-10°C and transported to the laboratory within 24 hours.

[0074] After arriving in the laboratory, enter the barcode and label into the computer, take out the placenta carefully with tweezers, ...

Embodiment 2

[0080] Example 2: Method for separating and extracting hematopoietic stem cells from placenta

[0081] The placenta came from the obstetrics and gynecology department of the local hospital. First, after obtaining the consent of the pregnant woman or her family to donate the placenta, check all the pregnant women’s examination reports and confirm that there is no virus, syphilis or blood-related viral infection, and then ask the pregnant woman’s family medical history, infection and infection history. After confirming that everything is normal, collect 5 mL of maternal blood, put the placenta and maternal blood into the placenta storage and transportation box, and affix the label and barcode. During the storage and transportation of the placenta, the temperature in the box is maintained at 4-10°C and transported to the laboratory within 24 hours.

[0082] Refer to the method in Example 1 to perform the initial inspection of the placenta, pre-sterilization of the placenta, and dete...

Embodiment 3

[0087] Example 3: Method for separating and extracting hematopoietic stem cells from placenta

[0088] The placenta came from the obstetrics and gynecology department of the local hospital. First, after obtaining the consent of the pregnant woman or her family to donate the placenta, check all the pregnant women’s examination reports and confirm that there is no virus, syphilis or blood-related viral infection, and then ask the pregnant woman’s family medical history, infection and infection history. After confirming that everything is normal, collect 5 mL of maternal blood, put the placenta and maternal blood into the placenta storage and transportation box, and affix the label and barcode. During the storage and transportation of the placenta, the temperature in the box is maintained at 4-10°C and transported to the laboratory within 24 hours.

[0089] Refer to the method in Example 1 to perform the initial inspection of the placenta, pre-sterilization of the placenta, and dete...

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Abstract

The invention provides a method for extracting hemopoietic stem cells from placentas, and especially relates to a method for extracting hemopoietic stem cells from placentas in a lavage manner. In one embodiment of the invention, the method comprises the following steps: 1, collecting the placentas; 2, carrying out initial inspection on the placentas; 3, pre-disinfecting the placentas; 4, detecting the placentas and maternal blood; 5, lavaging hemopoietic stem cells of the placentas; and 6, concentrating and purifying the hemopoietic stem cells. The method has the advantages presented in the specification, and a flow cytometer detection result of the hemopoietic stem cells obtained through the method shows that the hemopoietic stem cells have a very high percentage of CD34 positive cells.

Description

Technical field [0001] The invention relates to a method for separating and extracting hematopoietic stem cells from the placenta, in particular to a method for separating and extracting hematopoietic stem cells from the placenta by lavage. Background technique [0002] Hematopoietic stem cells refer to a type of cells with self-renewal and multidirectional differentiation capabilities. Its basic characteristic is self-renewal ability, that is, after a cell cycle activity, it can produce two hematopoietic stem cells with the same properties as before division, and at the same time have the ability of multidirectional differentiation, that is, under certain environmental conditions, hematopoietic stem cells have The ability to differentiate into various blood cells. [0003] Hematopoietic stem cell transplantation is currently widely used in malignant hematological diseases (such as acute leukemia, chronic myeloid leukemia, etc.), non-malignant refractory blood diseases (such as ap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0735
Inventor 许晓椿王正
Owner BOYALIFE
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