Method for increasing extraction rate of CD34 positive stromal vascular fraction (SVF) in high-fat tissue

A fat tissue extraction rate technology, applied in tissue culture, cell dissociation methods, bone/connective tissue cells, etc., can solve the problem of low yield of stem/progenitor cells
CN106337036APending Publication Date: 2017-01-18HANGZHOU S EVANS BIOSCI LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HANGZHOU S EVANS BIOSCI LTD
Publication Date
2017-01-18

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Abstract

The invention discloses a method for increasing the extraction rate of the CD34 positive stromal vascular fraction (SVF) in high-fat tissue. The method includes the steps that fat tissue is subjected to suspension with normal saline, centrifugation is carried out to remove an oil layer, sediment is repeatedly centrifuged, sediment obtained in the last time of centrifugation is crushed, and crushed tissue is obtained; then, a collagenase aqueous solution is added into the crushed tissue, oscillation is carried out at 37 DEG C for digestion, centrifugation is carried out after digestion is finished, and sediment, namely, the CD34 positive SVF is taken. By means of the method, low-concentration collagenase can be used for digestion, and the SVF containing high-ratio CD34 positive stem / progenitor cells is obtained from the fat tissue; the ratio of CD34 positive stem / progenitor cells in the SVF is larger than 24%, and more than 6.0*10<5> CD34 positive cells can be obtained from each milliliter of fat tissue.
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Description

[0001] (1) Technical field

[0002] The invention relates to the field of biotechnology, in particular to a method for efficiently preparing SVF from adipose tissue.

[0003] (2) Background technology

[0004] Current studies have found that adipose tissue is one of the sources of pluripotent cells, and these pluripotent cells exist in the stromal vascular fraction (SVF) of adipose tissue. SVF is a group of heterogeneous cell populations obtained after removing mature adipocytes from adipose tissue, and CD34-positive stem / progenitor cells are the most important functional cell population. Studies have shown that adipose-derived SVF not only has the multidirectional potential to differentiate into osteogenic, cartilage, fat, blood vessel and neuron-like cells, but also secretes various factors such as VEGF, bFGF, HGF, angiogenesis factor, angiopoietin 1, etc. This heterogeneity makes it uniquely advantageous in participating in the repair of tissue damage and promoting regenera...

Claims

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