Kit for cell culture

A kit, stem cell technology, applied in tissue culture, animal cells, vertebrate cells, etc., can solve the problem of stem cell adherence, maintenance of proliferation stability, etc. Unsatisfactory problems, to achieve the effect of maintaining proliferation ability and multi-directional differentiation potential, good proliferation ability and multi-directional differentiation potential, and eliminating the risk of spreading heterogeneous pathogens
CN105420186AInactive Publication Date: 2016-03-23郭镭 +2
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
郭镭
Publication Date
2016-03-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a novel kit for serum-free stem cell culture. The kit comprises a serum-free culture medium, wherein the serum-free culture medium contains 0.05-0.2 part of beta-mercaptoethanol, 0.5-2 parts of a non-essential amino acid aqueous solution, 4-6 parts of human mesenchymal stem cell culture supernatant concentrate, 90-95 parts of a-MEM / DMEM-F12 and human recombinant basic fibroblast growth factors with the final concentration of 5-15 ng / ml by volume. The culture supernatant concentrate is prepared by adopting the following steps of collecting the umbilical cord mesenchymal stem cell culture supernatant, centrifugally removing cells, cellular debris, impurities and the like, performing filtration by using a micro-filtration membrane and performing ultra-filtration concentration. By utilizing the kit for stem cell culture, the multi-directional differentiation potential and stronger proliferation ability of the cells are still kept under the condition of long-term culture.
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Description

technical field

[0001] The invention relates to the research field of stem cells, in particular to a novel, high-efficiency serum-free medium and its preparation method and application. Background technique

[0002] Mesenchymal stem cells are ubiquitous in various tissues and organs of the human body, have multi-directional differentiation potential, stimulate tissue regeneration, regulate immunity and other functions, and have broad application prospects in the field of cell therapy.

[0003] Bone marrow mesenchymal stem cells have been widely used clinically, and current research shows that umbilical cord-derived mesenchymal stem cells can not only become an ideal substitute for bone marrow mesenchymal stem cells, but also have greater application potential. Among them, human umbilical cord mesenchymal stem cells (humanUmbilicalCordmesenchymalstemcells, hUC-MSCs) derived from human umbilical cord express a variety of unique markers of embryonic stem cells, with high differ...

Claims

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