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Connexin core sequence-containing amphiphilic polypeptide and application thereof

An amphiphilic, fibrous material technology, applied in the medical and biological fields, which can solve the problems of poor biological activity, immunogenicity, and poor biodegradability

Inactive Publication Date: 2013-11-06
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although traditional scaffold materials such as chitosan, hyaluronic acid, fibrin, and collagen derivatives can promote cell adhesion, growth, and differentiation, they generally have high mechanical strength, poor biodegradability, and degradation products. It has the disadvantages of toxicity, poor biological activity and immunogenicity, which limit its application in intervertebral disc tissue engineering

Method used

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  • Connexin core sequence-containing amphiphilic polypeptide and application thereof
  • Connexin core sequence-containing amphiphilic polypeptide and application thereof
  • Connexin core sequence-containing amphiphilic polypeptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation of nanofiber material of the present invention

[0045] The nanofiber material of the present invention is composed of the structure of AcN-RADARADARADARADAGGDHLSDNYTLDHDRAIH-CONH 2 The amphipathic polypeptide of (DHL-PA) and the structure is AcN-RADARADARADARADA-CONH 2 (RADA-16-PA) amphiphilic polypeptide self-assembly.

[0046] DHL-PA (AcN-RADARADARADARADAGGDHLSDNYTLDHDRAIH-CONH) was synthesized by a solid-phase automatic peptide synthesizer 2 ) amphiphilic polypeptide, the purity of the polypeptide detected by high performance liquid chromatography is 97.02%, and the molecular weight of the polypeptide detected by mass spectrometer is 3730.92. For detailed parameters, see Figure 10 , 11 .

[0047] The concentrations of DHL-PA and RADA-16-PA amphiphilic polypeptide solutions are 0.25%-10% (m / v) according to the volume ratio of DHL-PA and (DHL-PA+RADA-16-PA) to 10%- 75% in Ca 2+ Triggered self-assembly to form composite nanofibrous materials (DHL-PA / R...

Embodiment 2

[0050] The difference from Example 1 is that the triggering conditions for self-assembly of DHL-PA and RADA-16-PA amphiphilic polypeptide solutions to form composite nanofiber materials are: self-assembly to form composite nanofibers under the trigger of cell culture medium DMEM-F12. fiber material. The rest are the same as in Example 1.

[0051] The following is a sequence listing of amino acids involved in the present invention, wherein: Sequence 1 is: the core sequence of linker protein linkN; Sequence 2 is: RADA-16 evolved from the EAKA16-II fragment of zuotin yeast protein; Sequence 3 is: provided by the present invention of amphipathic polypeptides containing the connexin core sequence.

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Abstract

The invention provides connexin core sequence-containing amphiphilic polypeptide AcN-RADARADARADARADAGGDHLSDNYTLDHDRAIH-CONH2, wherein the amphiphilic polypeptide and the amphiphilic polypeptide with the structure of AcN-RADARADARADARADA-CONH2 are triggered by Ca2+or a cell culture medium DMEM-F12 to automatically form into a compound, i.e. a nanometer-grade fiber material, and the nanometer-grade fiber material is taken as a bracket of the bone mesenchymal stem cells, so that the biological behavior of the cells can be obviously improved, the degenerated intervertebral disc can be repaired, and the original height can be recovered.

Description

Technical field: [0001] The present invention belongs to medicine and biotechnology, and relates to an amphiphilic polypeptide containing a connexin core sequence and its application in medicine. Background technique: [0002] Intervertebral disc degeneration (IVD) is the leading cause of low back pain, and studies show that 60%-80% of people will experience low back pain at least once in their lifetime. The main manifestation of intervertebral disc degeneration is that the water content of the nucleus pulposus decreases, the tension of the nucleus pulposus decreases, and the height of the intervertebral disc decreases. At the same time, hyaluronic acid and keratin sulfate decreased, low-molecular-weight glycoproteins increased, fibrillar degeneration and collagen fiber deposition increased, the nucleus pulposus lost elasticity, and the intervertebral disc structure was relaxed. Once this degeneration occurs, it will fall into a vicious circle and further aggravate the dege...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00A61L27/22D01F8/02
Inventor 邵增务吴永超王佰川杨述华张志才
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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