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Cell freezing medium

A cryopreservation solution and cell technology, applied in the field of cell and bioengineering, can solve the problems of protein denaturation, cytotoxicity, etc., and achieve the effect of consistent shape, high survival rate and good proliferation ability

Active Publication Date: 2018-12-14
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the refrigerants currently used, dimethyl sulfoxide (DMSO) is the most commonly used refrigerant in cell preservation, but it also has certain toxicity to cells at the same time, and can denature intracellular proteins at room temperature. Therefore, these deficiencies limit its further application

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0019] A cell cryopreservation solution, the basic component of which is RPMI-1640 medium, and the added components are as follows:

[0020] Glycerin (glycerinum) 10v / v%;

[0021] Fetal calf serum 10v / v%;

[0022] Sodium alginate 0.10w / v%.

Embodiment 2

[0024] A cell cryopreservation solution, the basic component of which is DMEM-F12 medium, and the added components are as follows:

[0025] Glycerin 10v / v%;

[0026] Fetal bovine serum 10v / v%;

[0027] Sodium Alginate 0.01w / v%.

Embodiment 3

[0029] A cell cryopreservation solution, the basic component of which is RPMI-1640 medium, and the added components are as follows:

[0030] Glycerin 10v / v%;

[0031] Fetal bovine serum 10v / v%;

[0032] Sodium hyaluronate (Hyaluronic acid sodium salt) 0.10w / v%.

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Abstract

The invention discloses a cell freezing medium. The cell freezing medium comprises a basal culture medium, glycerin, fetal calf serum and a non-permeable protective agent, and the contents of the components are 80-94 v / v% of the basal culture medium, 5-10 v / v% of glycerol, 1-10 v / v% of the fetal calf serum and 0.01-0.10 w / v% of the non-permeable protective agent, and the basal culture medium comprises RPMI-1640, MEM, high-sugar DMEM, low-sugar DMEM or DMEM-F12; and the non-permeable protective agent comprises sodium alginate, sodium hyaluronate or gamma-polyglutamic acid. Dimethyl sulfoxide isnot contained, a good freezing effect is achieved through different matching of the non-permeable protective agent with glycerin and fetal calf serum, a strong water molecule complexing ability is achieved, the freezing function is similar to the freezing function of dimethyl sulfoxide, but formation of intracellular ice crystals is reduced during freezing; the cell freezing medium has clear added ingredients, high safety, small damage to cells, consistent morphology before and after freezing, a high cell survival rate after cell resuscitation and good proliferative capacity.

Description

technical field [0001] The invention relates to the technical field of cells and bioengineering, in particular to a cell cryopreservation solution. Background technique [0002] Cell cryopreservation is one of the important links in biological research. It freezes artificially cultured cells, prevents cell degradation caused by cell subculture, prevents bacterial contamination in subculture, and solves a series of problems in the process of maintaining subculture. Questions matter. However, cryopreservation of cells can also damage cells to a certain extent. [0003] At present, in the field of cell cryopreservation, cryogens (English full name Cryo-Protectant Agent, CPA for short) are widely used. Described refrigerant comprises osmotic refrigerant and non-permeable refrigerant, and wherein, osmotic refrigerant mainly comprises dimethyl sulfoxide, glycerin, ethylene glycol, propylene glycol, acetamide and methyl alcohol; Its protection mechanism is: in Before the cell fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 蔡海波陈焕芸周乐豪谭文松
Owner EAST CHINA UNIV OF SCI & TECH
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