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Method for extracting lipid from Chlorella sorokiniana CS-01

An extraction method and technology of chlorella, applied in the direction of fat production, fat oil/fat production, etc., can solve the problem of less application of cell crushing technology

Inactive Publication Date: 2011-09-28
CENT SOUTH UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] Compared with other technologies, the extraction technology after cell crushing has the advantages of economy, high efficiency, and high extraction efficiency, and has been widely used in the extraction of oil crop lipids, but the application of cell crushing technology in microalgae lipid extraction is relatively seldom

Method used

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  • Method for extracting lipid from Chlorella sorokiniana CS-01
  • Method for extracting lipid from Chlorella sorokiniana CS-01
  • Method for extracting lipid from Chlorella sorokiniana CS-01

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Embodiment 1

[0025] 1. Cultivation, collection and drying of algal species

[0026] Chlorella sorokiniana CS-01 (CCTCC M 209220), culture conditions: BG-11 medium (published formula), light intensity 70μmol photons m -2 the s -1 , photoperiod 10:14 (L / D), temperature 25±1°C, air flow 2.817vvm (use 0.22μm filter head, filter to sterilize). The growth curve (OD) was determined by absorbance photometry. 680 ), cultivated to the middle and late stages of logarithmic growth, collected the algal bodies by centrifugation at 6000rpm for 10 minutes, and washed the algae bodies twice with sterile water. The algae mud was vacuum freeze-dried for 8 hours, and stored in a desiccator for future use.

[0027] 2. Ultrasonic crushing

[0028] Chloroform / methanol volume ratio (1:2), solid-liquid ratio (1g:36ml) vibration extraction (15-45s), ultrasonic crushing (440W, 180s), then add 1 / 2 times the volume of water and 1 / 3 Doubling the volume of chloroform solution, ultrasonic crushing (440W, 180s), and ...

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Abstract

The invention discloses a method for extracting lipid from Chlorella sorokiniana CS-01 (CCTCC M209220), namely an ultrasonic-assisted chloroform / methanol extraction method. The method mainly comprises the following steps of: collecting algae, drying, extracting by using chloroform / methanol, performing ultrasonic crushing, washing, and drying. Through the certification, compared with a Soxhlet extraction method, an acid heat extraction method, and a chloroform / methanol extraction method, the method has the advantages that: the extraction ratio of the lipid is improved by 1.4, 1.7 and 0.5 timesrespectively; meanwhile, a lipid extraction phase is positioned under a cell debris solid phase, so the method is favorable for the subsequent direct separation of the lipid, and meets the requirements of mass industrialized production.

Description

Technical field: [0001] The invention relates to a method for extracting lipid from Chlorella sorokiniana CS-01 (CCTCC M209220). Background technique: [0002] Global warming and the oil crisis have given birth to the development of a low-carbon economy, and the research on alternative energy has become urgent. As a new type of green energy, microalgae biodiesel can reduce CO 2 The advantages of low emission, high lipid content, high yield, and small land occupation area have become the research hotspots of countries all over the world. The main process of microalgae biodiesel production includes: microalgae breeding, microalgae cultivation, microalgae harvesting, lipid extraction and conversion process. Each unit is very important, and the cost of lipid extraction is an important factor affecting its large-scale industrial production. [0003] The cell wall of microalgae is tough, and the cell body is smaller than that of oil crops, so the traditional pressing method and...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/10
Inventor 夏金兰邱冠周巩三强王斌梁闫艺瀛聂珍媛
Owner CENT SOUTH UNIV
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