The invention relates to the biological field, and especially relates to a substrate, a method and a kit for in-vitro
quantitative determination of the activity of vWF-CP
enzyme. The substrate comprises a vWF functional area
amino acid sequence. The end N and the end C of the vWF functional area
amino acid sequence are respectively combined with a HIS
label amino acid sequence and a FLAG
label amino acid sequence. The method comprises the following steps: the holes of an ELISA plate are coated with an
antibody in advance; and then, the substrate, a sample to be tested, an ELISA
antibody, and
chromogenic agent are added, and the
absorbance of liquid in the holes of the ELISA plate is measured using an ELISA reader. The kit comprises an ELISA plate, a substrate,
diluent, washing liquid,
buffer solution, an
enzyme-labeled
antibody,
chromogenic solution and stop solution, or comprises an ELISA plate, an antibody, a substrate,
diluent, washing liquid,
buffer solution, an
enzyme-labeled antibody,
chromogenic solution and stop solution. The substrate, the method and the kit for in-vitro
quantitative determination of the activity of vWF-CP enzyme have the advantages of high sensitivity, high accuracy, simple operation and low price, and are suitable for broad
patient screening.