Application of AGP1, SERPINA3 and CDH1 content detection system in screening active tuberculosis patients

Abstract

The invention discloses application of an AGP1, SERPINA3 and CDH1 content detection system in screening active tuberculosis patients. The inventor of the application discovers that three proteins with specific expression, namely AGP1, SERPINA3 and CDH1, in the active tuberculosis patients and establishes an active tuberculosis patient screening model with sensitivity of 81.18%, specificity of 96.15% and accuracy of 88.17% by use of content of three proteins in plasma. By use of the content of three proteins in plasma and the active tuberculosis patient screening model, the active tuberculosis patients can be screened, and active tuberculosis patient screening products can be prepared by use of substances for detecting the content of three proteins in plasma.

Description

technical field [0001] The invention relates to the application of a system for detecting the contents of AGP1, SERPINA3 and CDH1 in the screening of active tuberculosis patients in the field of biotechnology. Background technique [0002] Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis (MTB) infection, which seriously threatens human health. The fifth national tuberculosis epidemiological survey shows that there are more than 5 million adults with active pulmonary tuberculosis in China. After adults are infected with Mycobacterium tuberculosis, some of them are living with the bacteria and will not develop tuberculosis, which is called latent tuberculosis infection (LTBI); about 5-10% of latent infections will continue to develop into active tuberculosis ( Active tuberculosis, ATB). Active pulmonary tuberculosis refers to a person with positive sputum smear, which proves that Mycobacterium tuberculosis has been excreted, and the lesi...

AI Technical Summary

Problems solved by technology

Although the sputum smear staining method can produce results on the same day, it cannot distinguish tuberculosis bacilli from non-tuberculous mycobacteria, nor can it distinguish whether MTB is a living or dead bacteria

Although the sensitivity of Mycobacterium tuberculosis culture method is higher, it takes a long time, and it takes 1 month to get the result even for rapid culture.

In addition, for a large number of clinically active tuberculosis patients with extrapulmonary tuberculosis and smear-negative or bacterium-negative patients, the application of gold standard detection methods is limited, which exacerbates the difficulty of diagnosis and brings resistance to timely treatment.

Although some advanced nucleic acid amplification-based molecular biology detection technologies (such as XpertMTB / RIFassay) have emerged in recent years, they cannot be fully promoted due to the limitations of equipment and diagnostic costs, as well as the high false positive rate.

Therefore, none of the current tuberculosis diagnostic methods or auxiliary diagnostic methods can achieve rapid and effective differential diagnosis of latent infection of Mycobacterium tuberculosis and active tuberculosis, which makes clinical problems such as serious treatment delays and overtreatment

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