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Novel use of adipogenic differentiation-associated proteins as screening markers for HBV-HCC patients

A technology related to protein and fat is applied in the field of adipose differentiation-related protein as a screening marker for HBV-HCC patients, which can solve the problems of inability to distinguish abnormal masses or masses, and low detection rate of early liver cancer.

Inactive Publication Date: 2016-11-09
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Imaging studies can be used to locate specific areas of abnormal tissue in the liver, but cannot distinguish tumors from other abnormal masses or masses of tissue (nodules) in the liver
AFP is an important indicator for the diagnosis and monitoring of HCC, but only 50%-75% of patients with primary liver cancer have abnormally high serum levels of AFP, and its detection rate for early liver cancer is not high

Method used

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  • Novel use of adipogenic differentiation-associated proteins as screening markers for HBV-HCC patients
  • Novel use of adipogenic differentiation-associated proteins as screening markers for HBV-HCC patients
  • Novel use of adipogenic differentiation-associated proteins as screening markers for HBV-HCC patients

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. The process of discovering that fat differentiation-related proteins can be used as markers for screening hepatocellular carcinoma

[0021] Paired samples: 5 cases of HBx transgenic mice of different ages, and littermates of wild normal mice.

[0022] The mouse liver that had been internally standardized by SILAC was used as the internal standard protein, and quantitative proteomics UPLC-LTQOrbitrap-Velos ultra-high pressure liquid chromatography and mass spectrometry were used to analyze the protein expression changes in the liver tissue of HBx transgenic mice at different ages; The search engine (version 4.0.4) searches the Swiss-Prot (version 20110710) database and selects the analysis strategy incorporating internal standards. Classic card value: protein abundance increased by 1.0 times or protein abundance decreased by 1.0 times. By manually detecting the mass spectrum, a quantitative peptide of ADFP protein (GAVTGSVERTK) was selected, and it was found...

Embodiment 2

[0024] Example 2, ADFP Level Differences in Cancer Tissues and Paracancerous Tissues

[0025] Paired samples: cancer tissues and paired paracancerous tissues of 79 HBV-HCC patients;

[0026] Cancer tissues and matched paracancerous tissues of 11 non-HBV-HCC patients.

[0027] All patients were volunteers who gave informed consent.

[0028] Each sample was taken separately for immunohistochemical detection. The primary antibody used was rabbit anti-ADFP antibody from EPITOMICS Company, Cat. No. 3293-1. The immunohistochemistry kit used SP Rabbit&Mouse HRP Kit Rabbit / Mouse Universal Streptavidin-HRP kit from Kangwei Century Biotechnology Co., Ltd., catalog number CW0120A. DAB color reaction was used. For the results of an HBV-HCC patient and a non-HBV-HCC patient, see figure 2 (A is a 100 times magnified unit photo, B is a 200 times magnified photo). Image-Pro Plus6.0 software was used to scan and analyze the optical density value of the photos of cancer tissue and adjace...

Embodiment 3

[0030] Embodiment 3, the level difference of ADFP in the serum of different populations

[0031] Five groups of people: 10 normal people, 10 patients with chronic hepatitis C, 10 patients with hepatitis B, 10 patients with HBV-HCC, and 10 patients with drug-induced acute liver injury, all volunteers with informed consent.

[0032] Serum from each volunteer was collected, subjected to 10% polyacrylamide gel electrophoresis, and then western blot. The primary antibody used was rabbit anti-ADFP antibody from EPITOMICS (Cat. No. 3293-1). The secondary antibody used was horseradish peroxidase-labeled mouse anti-rabbit IgG from KPL (Product No. 474-1506). Chemiluminescence Reagent Wwestern Plus-ECL is a product of PerkinElmer (Cat. No. 203-12381). A normal person (lane 1), a hepatitis C patient (lane 2), a hepatitis B patient (lane 3), an HBV-HCC patient (lane 4), a drug-induced acute liver injury For patient (lane 5) results see Figure 4 .

[0033] Use Bandscan5.0 software t...

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Abstract

The invention discloses a novel application of adipose differentiation related protein as the HBV-HCC patient screening marker. The invention demands to protect the application of adipose differentiation related protein (ADFP) in preparation of kits for screening HBV-HCC patients. The invention also demands to protect the application of ADFP in preparation of kits for detecting or co-detecting whether a patient is a HBV-HCC patient or not. The invention also protects a kit for screening HBV-HCC patients, and the kit comprises a substance for detecting ADFP. The invention also protects a kit for detecting or co-detecting whether a patient is a HBV-HCC patient or not, and the kit comprises a substance for detecting ADFP. ADFP can be used to screen HBV-HCC patients so as to give an early warning, moreover, the result is precise, the operation is convenient, and thus the ADFP has a practical value.

Description

technical field [0001] The invention relates to a new application of fat differentiation-related protein as a screening marker for HBV-HCC patients. Background technique [0002] Hepatocellular carcinoma (HCC) is one of the malignant tumors worldwide, with high morbidity and mortality. About 650,000 people die from HCC every year, and more than 50% of them occur in China. The incidence rate shows an increasing trend year by year. 90% of HCC patients in China have a history of hepatitis B virus (HepatitisB virus, HBV) infection. According to whether they carry hepatitis B virus, HCC patients can be divided into HBV-HCC patients (full name is hepatitis B virus-associated hepatocellular carcinoma, that is, patients with hepatocellular carcinoma carrying hepatitis B virus) and non-HBV-HCC patients (full name is non-hepatitis B Virus-associated hepatocellular carcinoma, that is, patients with hepatocellular carcinoma who do not carry the hepatitis B virus). Drug chemotherapy, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/574G01N33/68
Inventor 徐平徐忠伟翟琳辉衣泰龙樊锋旭李衍常李宁常蕾
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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