Preparation method for plant mutant
A technology for mutants and plants, which is applied in the field of preparation of plant mutants, and can solve problems such as the inability to realize mass reproduction of sterile line seeds
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[0046] The present invention is described in detail below in conjunction with embodiment. In order to make the purpose, technical solutions and advantages of the present invention clearer and clearer, the present invention will be further described in detail below, but the present invention is not limited to these examples.
[0047] In order to solve the above-mentioned technical problems, the present invention provides a method for preparing a plant mutant, comprising the following steps:
[0048] T1. Construct a CRISPR / cas9 gene knockout vector to knock out the CYP78A13 gene;
[0049] T2. Agrobacterium transformation;
[0050] T3. Gene knockout detection;
[0051] T4. Phenotype identification of transgenic plants.
[0052] The step T1. constructing the CRISPR / cas9 gene knockout vector, knocking out the CYP78A13 gene, the sgRNA sequence is as follows:
[0053] 5'-ggcaCACAATGGCTCCAGCATTCC-3'
[0054] 5'-AAACGGAATGCTGGAGCCATTGTG-3'.
[0055]In order to solve the above-men...
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