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Cell development map and development marker gene of mandible tissue of human four-month-old embryo

A mandibular, cellular technology, applied in the direction of genomics, proteomics, microbial determination/inspection, etc.

Inactive Publication Date: 2022-05-27
AFFILIATED HOSPITAL OF NANTONG UNIV
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Problems solved by technology

[0005] However, the developmental map of human embryonic mandibular tissue has not yet attracted attention and has not been reported. Therefore, the construction of a cell developmental map of human four-month-old embryonic mandibular tissue will play an important role in the study of the growth and development of the mandible.

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  • Cell development map and development marker gene of mandible tissue of human four-month-old embryo
  • Cell development map and development marker gene of mandible tissue of human four-month-old embryo
  • Cell development map and development marker gene of mandible tissue of human four-month-old embryo

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Embodiment Construction

[0023] In order to make the technical problems, technical solutions and advantages to be solved by the present invention more clear, the following will be described in detail with reference to specific embodiments.

[0024] The invention performs single-cell sequencing on human four-month-old embryo mandibular tissue, uses UMAP (Uniform Manifold Approximation and Projection for Dimension Reduction, uniform manifold approximation and projection algorithm) to reduce dimensionality into groups, and utilizes Marker gene (cell group characteristic expression gene) ) annotated cell populations and constructed a cellular developmental map of human embryonic mandibular tissue. The specific steps are:

[0025] 1. Preparation of single cell suspension

[0026] The mandibular tissue was washed three times with Hanks equilibration solution, cut into 1-2 mm tissue pieces, and placed in 2 ml GEXSCOPE. TM (Singleron Biotechnologies) tissue dissociation solution was digested at 37°C for 15 ...

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Abstract

The invention discloses a cell development map of mandible tissues of human four-month-old embryos. Comprising 19 cell populations including endothelial cells, Schwann cells, proliferating cells, satellite cells, muscle cells, red blood cells, myoblasts, transformed cells 3, transformed cells 1, mesenchymal stem cells, monocytes, osteoclasts, transformed cells 2, neural crest cells, osteoblasts, epithelial cells, chondrocytes, pericytes and tendons. The invention further discloses a construction method of the cell development map of the mandible tissue of the human four-month-old embryo, and the cell development map of the mandible tissue of the human four-month-old embryo is constructed by carrying out single cell sequencing on the mandible tissue of the human four-month-old embryo, carrying out UMAP dimension reduction grouping and annotating a cell population by utilizing a Marker gene. The invention provides a complete and comprehensive cell development map of mandible tissues of human four-month-old embryos, and provides new basic data for researching the growth and development of mandible.

Description

technical field [0001] The invention belongs to the technical field of biological sciences, and relates to a cell developmental atlas and developmental marker genes of human four-month-old embryonic mandibular tissue, in particular to a cell developmental atlas of human four-month-old embryonic mandibular tissue, a four-month-old human embryonic The application of cell development marker genes of mandibular tissue and human four-month-old embryonic mandibular tissue in judging the developmental stage of embryonic mandibular tissue. Background technique [0002] Currently, research in developmental biology is mainly based on model organisms. Human embryonic development (from fertilized egg to fetal birth) remains a poorly understood "black box" due to practical challenges. Recently, a joint study from multinational research institutions elaborated the roadmap for the construction of the Human Developmental Cell Atlas and the Reference Map during Pregnancy. The related result...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6869G16B20/00
CPCC12Q1/6888C12Q1/6869G16B20/00C12Q2535/122
Inventor 冯兴梅谭妙盈朱谦齐李立人孙旭奎韩剑鹏李磊
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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