Application of carbon monoxide molecules in inhibition on acute rejection after skin grafting

A technique for skin grafting, acute rejection

Inactive Publication Date: 2012-08-01
AFFILIATED HOSPITAL OF JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limitations of non-specific anti-immune rejection, many fatal complications will appear in the case of long-term administr

Method used

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  • Application of carbon monoxide molecules in inhibition on acute rejection after skin grafting
  • Application of carbon monoxide molecules in inhibition on acute rejection after skin grafting
  • Application of carbon monoxide molecules in inhibition on acute rejection after skin grafting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Experimental observation of exogenous carbon monoxide releasing molecule CORM-2 on grafts after skin transplantation in allogeneic mice Test method:

[0023] (1) Animal and allogeneic mouse skin graft models

[0024] Clean-grade ICR mice and BALB / C mice were used as donors and recipients to establish allogeneic skin transplantation models, which were divided into 5 groups: 1 group of sham operation group, 2 groups of allogeneic skin transplantation group, and 3 groups of allogeneic skin transplantation Allogeneic skin transplantation + CORM-2 group, 4 allogeneic skin transplantation + iCORM-2 groups, 5 allogeneic skin transplantation + CsA groups; postoperative administration was given according to the requirements of each group, and the degree of rejection of the grafted skin was observed. The observation period was 7 days. At the end of the observation period, the grafted skin pieces were removed and fixed with 4% paraformaldehyde, embedded in paraffin, sectioned, ro...

Embodiment 2

[0058] Effects of exogenous carbon monoxide releasing molecule (CORM) 2 on spleen lymphocyte function after skin transplantation in allogeneic mice

[0059] Acute rejection after allogeneic skin transplantation in mice is mainly characterized by monocyte-mediated cellular immunity dominated by T lymphocytes. Among them, the ratio of the two subsets of T lymphocytes (CD4 + / CD8 + ) has been recognized to a certain extent in reflecting the degree of immune rejection of transplantation. CD4 + T cells are helper T lymphocytes, which are the main cells in the process of immune response to transplantation, and CD8 + T is cytotoxic T lymphocytes, which are effector cells in the process of transplantation immune response and play a certain role in regulating immune cells. CD4 + / CD8 + The increase of the ratio is closely related to the degree of acute rejection, so it can be used as an important index to measure the degree of rejection.

[0060] In addition, after transplantati...

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Abstract

The invention discloses an application of carbon monoxide molecules in inhibition on acute rejection after skin grafting. According to a large number of cell tests, the inventor discovers that after the carbon monoxide molecules are dissolved into a dimethylsulfoxide (DMSO) solvent and placed into an organism, CO can be continuously released, so the carbon monoxide molecules can serve as exogenous CO donors. The skin of ICR mice and BALB/C mice can serve as a donor and a receptor; an allogeneic gene acute rejection model can be established; exogenous CO is injected into the caudal veins of the mice; and early intervention and the test are performed, so that the carbon monoxide release molecules have inhibition effect on the acute rejection after skin grafting. The carbon monoxide release molecules are used for inhibiting the acute rejection after skin grafting; the concentration is controlled relatively easily and correctly; during long-term use, the carbon monoxide release molecules are added conveniently; and the carbon monoxide molecules can serve as an immuno-inhibitor with high specificity, low toxicity and high efficiency.

Description

technical field [0001] The patent of the present invention relates to the new application of carbon monoxide releasing molecules in immunosuppression, and specifically relates to a method for early application of carbon monoxide releasing molecules to suppress acute rejection after skin transplantation. Background technique [0002] Allotranspantation is currently the main way to treat end-stage organ failure. As a convenient means of organ transplantation, allogeneic skin transplantation is not only widely used in the research of anti-graft rejection, but also solves the ethical problems caused by the lack of autologous skin and xenogeneic skin transplantation in clinical practice to a certain extent. However, in individuals who have received allogeneic tissue and organ transplantation, the MHC antigens of the grafts are recognized by the autoimmune system, resulting in rejection. As early as the 18th century, humans began to explore skin allografts. Initial attempts were...

Claims

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Application Information

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IPC IPC(8): A61K33/00A61P37/06
Inventor 孙炳伟孙志伟孙艳
Owner AFFILIATED HOSPITAL OF JIANGSU UNIV
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