The invention discloses a method for preparing alpha, beta-acetylenic ketone compounds through carbon monoxide-releasing molecular carbonylation carbon-carbon bond coupling. According to the method, CO gas is not used, and the method comprises the following steps: by taking palladium chloride as a catalyst, taking 4,5-bis (diphenylphosphino)-9,9-dimethyl xanthenes as a ligand, taking N, N-dimethyl formamide as a solvent, taking pentacarbonyl iron as a CO supply source of a carbon monoxide-releasing molecule, coupling aryl halides and terminal alkyne under alkaline conditions, thereby obtaining the alpha, beta-acetylenic ketone compounds. According to the method disclosed by the invention, usage of high-pressure poisonous gas carbon monoxide is avoided, and the method is simple and safe in experimental operation, mild in conditions and high in substrate applicability and can be widely applied to preparing the alpha, beta-acetylenic ketone compounds.