Detection method for DNA repair-related genes which respond to low-level radiation

Abstract

Disclosed is a detection method for DNA repair-related genes which respond to low-level radiation, which includes the steps of (1) breeding AKR / J mice, as a thymus cancer model, and normal ICR mice in a low-dose radiation environment; (2) collecting thymuses from the AKR / J thymus cancer mouse model and the normal ICR mice which have been bred at the step (1); (3) analyzing genes in the thymuses collected at the step (2); (4) detecting, among the genes analyzed at the step (3), a DNA repair-associated gene expressed commonly or differentially in the AKR / J thymus cancer mouse model and the normal ICR mice; and (5) amplifying the gene detected at the step (4) and measuring the expression level thereof.

Description

TECHNICAL FIELD[0001]The present invention relates to a detection method for DNA repair-associated genes responsive to low-dose radiation, and more particularly, to a method comprising exposing AKR / J mice, as a thymus cancer model, and normal ICR mice to low-dose radiation, collecting thymuses from the mice, identifying DNA repair-associated genes that are expressed commonly or differentially in response to radiation exposure, and amplifying the genes to measure expression levels thereof.BACKGROUND ART[0002]A variety of strategies have been applied to identify specific genes which are sensitive to low-dose radiation (0.7 mGy / h), but gene expression at the protein level has not been confirmed. In contrast, the genes identified in the present invention are revealed by differences in gene expression at the RNA level as well as at the protein level. Also, no attempts have been made to compare the expression patterns of genes associated with DAN damage and repair between healthy mice and...

AI Technical Summary

Benefits of technology

[0019]As described above, the present invention provides a method of detecting a DNA repair-associated gene which responds to low-dose radiation, comprising the steps of (1) breeding AKR / J mice, as a thymus cancer model, and normal ICR mice in a low-dose radiation environment; (2) collecting thymuses from the AKR / J thymus cancer mouse model and the normal ICR mice which have been bred at the step (1); (3) analyzing genes in the thymuses collected at the step (2); (4) detecting, among the genes analyzed at the step (3), a DNA repair-associated gene expressed commonly or differentially in the AKR / J thymus cancer mouse model and the normal ICR mice; and (5) amplifying the gene detected at the step (4) and measuring the expression level thereof. Thereby, the present invention has the following effects: the genes have the potential for use as genetic markers for DNA repair in response to low-dose radiation, making it possible to evaluate the relationship between radiation exposure and carcinogenesis for industrial and medical workers; the genes can be applied as indicators for DNA repair in response to low-dose radiation for evaluating cancer progress and the effects of radiation therapy for cancer patients; and the genes can be applied to assess the dose-response relationship between radiation doses and cancer incidence.

Problems solved by technology

Also, no attempts have been made to compare the expression patterns of genes associated with DAN damage and repair between healthy mice and cancer-induced mice, both of which are subjected to low doses of radiation.

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