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Method of applying procyanidins from lotus seed pots to prevent brain oxidative stress injury caused by extremely low-frequency electromagnetic fields (ELF-EMF)

A technology of proanthocyanidins and oxidative stress, applied in the field of medicine

Inactive Publication Date: 2013-06-19
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been confirmed that LSPCs have strong free radical scavenging and antioxidant activity in vivo and in vitro, can improve the learning and memory ability of aging and various aging model animals, anti-tumor, improve cardiovascular function, etc., and proanthocyanidins are recognized as strong antioxidants and free radicals scavenger, but did not involve its study on ELF-EMR-induced brain oxidative stress injury learning and memory

Method used

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  • Method of applying procyanidins from lotus seed pots to prevent brain oxidative stress injury caused by extremely low-frequency electromagnetic fields (ELF-EMF)
  • Method of applying procyanidins from lotus seed pots to prevent brain oxidative stress injury caused by extremely low-frequency electromagnetic fields (ELF-EMF)
  • Method of applying procyanidins from lotus seed pots to prevent brain oxidative stress injury caused by extremely low-frequency electromagnetic fields (ELF-EMF)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 The preventive effect of lotus pod proanthocyanidins on the learning and memory of mouse brain oxidative stress damage caused by extremely low frequency electromagnetic field

[0026] 1 Experimental materials

[0027] 1.1 Test sample

[0028] The lotus pod proanthocyanidins are prepared with reference to the Chinese authorized patent (ZL02115423.6). Fresh lotus pod raw materials, remove lotus seeds, clean, add sodium nitrite with a solvent volume of 0.5-1‰, seal and extract with boiling water, filter the extract, pass the filtrate through a macroporous absorbent resin column, wash with water to remove impurities that are not adsorbed Finally, wash the resin column with 70% ethanol by volume to obtain ethanol eluted fractions, concentrate under reduced pressure to remove ethanol, and freeze-dry to obtain brown lotus pod proanthocyanidin extract powder. The proanthocyanidin product has a proanthocyanidin content greater than 90%. The molecular weight range of...

Embodiment 2

[0060] Example 2 Preventive effect of lotus pod proanthocyanidins on oxidative stress injury of hippocampal neuron cells induced by extremely low frequency electromagnetic field

[0061] 1 Materials and methods

[0062] 1.1 Lotus pod proanthocyanidins (self-made in this laboratory, purity > 95%), the same as before.

[0063] 1.2 Experimental animals: Newborn SD suckling mice within 24 hours, provided by the Experimental Animal Center of Jiangsu University.

[0064] 1.3 Main reagents: High-glucose DMEM medium was purchased from GIBCO; acridine orange, ethidium bromide, and trypsin were purchased from Sigma; fetal bovine serum, B27 additive, cytarabine, L-polylysine, four Methyl azolium salt (MTT), dimethyl sulfoxide, Hoechst 33342, and ROS kit were all purchased from Beyontian Bioengineering Company.

[0065] 1.4 Instruments and equipment: Multiskan SPectrum full-wavelength microplate reader, Thermo Electric Company of the United States; SPectra MaxGemini fluorescent micropla...

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Abstract

The invention discloses a method of applying procyanidins from lotus seed pots to prevent brain oxidative stress injury caused by extremely low-frequency electromagnetic fields (ELF-EMF) and relates to the field of medical technology. The method includes the steps of adopting ICR mice to carry out animal experiments, utilizing a Morris water maze method to measure the influence of the procyanidins from the lotus seed pots on learning capacity and memory capacity of the mice and brain antioxidase systems of the mice due to the ELF-EMF and measure the content or the activity of lipid peroxidation products, nitric oxide and nitric oxide synthase in brain tissues and hippocampus of the mice, measuring the influence of the procyanidins from the lotus seed pots on the cell survival rate, cell survival forms, the content of antioxidase, the content of reactive oxide radicals, the content of the nitric oxide and the content of nitric oxide synthase through research of an in-vitro cultured hippocampal neuron cell damage model caused by the ELF-EMF. The result proves that the procyanidins from the lotus seed pots have a remarkable preventive effect on learning and memory deterioration due to the brain oxidative stress injury caused by the ELF-EMF in vitro and in vivo.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of lotus pod proanthocyanidins in the preparation of medicines or health food for preventing brain oxidative stress damage caused by extremely low-frequency electromagnetic fields. Background technique [0002] With the rapid development of electromagnetic technology, a large number of related products have flooded into every family and society. While generating huge economic benefits and bringing many conveniences, electromagnetic pollution and hazards that cannot be ignored have also emerged. Extremely low-frequency electromagnetic fields (ELF-EMF) are non-ionizing radiation emitted by electromagnetic waves with a frequency spectrum of 0-300 Hz, mainly due to non-thermal effects, and are mainly generated by power supply equipment, computers, mobile phones and various household appliances. One of the electromagnetic fields most closely related to our daily life ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/353A61P25/00
Inventor 张海晖段玉清王志高武妍张瑞马海乐
Owner JIANGSU UNIV
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