Detection method for oxidative stress functions of different organs of mice

A technology of oxidative stress and detection methods, applied in the field of new species of vertebrates, can solve the problems of lack of toxicological data on the structure of quantum dots, the mechanism of biological toxicity is not completely clear, and the research on the toxicity of quantum dots has a long way to go.

Pending Publication Date: 2019-01-04
XINXIANG MEDICAL UNIV
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Problems solved by technology

Due to the diversity of quantum dot structures and the lack of toxicological data, the mechanism of quantum dot toxicity to organisms is not yet fully understood.
Therefore, this requires researchers to study the toxicity and mechanism of biological materials fro...

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  • Detection method for oxidative stress functions of different organs of mice
  • Detection method for oxidative stress functions of different organs of mice
  • Detection method for oxidative stress functions of different organs of mice

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[0023] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0024] Quantum dots have great application prospects in the fields of inventions such as medicine and life sciences. With the continuous expansion of the application range of quantum dots, their biological safety has gradually attracted widespread attention. Design different particle sizes, different concentrations, and different exposure times to discover the effects of CdSe / ZnS quantum dots on the oxidative stress of different organs in vivo. Observe the effects of quantum dots on DNA damage, liver and kidney antioxidant enzyme-related genes and proteomes in mice, and further reveal the molecular toxicity effects of Cd...

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Abstract

The invention belongs to the technical field of new species of vertebrates, and discloses a detection method for oxidative stress functions of different organs of mice. The clean male ICR mice with the weight of 20 g are selected, the mice are randomly grouped, and CdSe/ZnS QDs establishes a poisoned mouse model through tail intravenous injection; low-dosage groups, middle-dosage groups and high-dosage groups of quantum dots QD605 are prepared, and control groups, low-dosage groups, middle-dosage groups and high-dosage groups of quantum dots QD525 are prepared; all the control groups are treated by normal saline; the exposure times of the dosage groups are 11 days, 22 days and 33 days respectively. According to the method, by observing the influence of the quantum dots on genes and proteomes relevant to DNA impairment of the mice and liver and kidney antioxidase, the molecular toxicity effect of the CdSe/ZnS quantum dots on the animal bodies is further disclosed, and basic materials are provided for toxicology invention and bio-safety evaluation of the quantum dots.

Description

technical field [0001] The invention belongs to the technical field of new varieties of vertebrates, and in particular relates to a method for detecting oxidative stress in different organs of mice. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: Quantum dots have great application prospects in the fields of inventions such as medicine and life sciences. However, with the continuous expansion of the application range of quantum dots, their biological safety has gradually attracted widespread attention. Due to the diversity of quantum dot structures and the lack of toxicological data, the toxic mechanism of quantum dots is still not fully understood. Studies have shown that quantum dots can be distributed in various systems in organisms and gather in certain tissues and organs, which are potentially toxic to organisms. The toxicity of quantum dots to cells and organisms is closely related to the type, size, ...

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Application Information

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IPC IPC(8): G01N33/50
CPCG01N33/5014G01N33/5088
Inventor 张秀华范雪晖王红霞陈素娟毕佳佳
Owner XINXIANG MEDICAL UNIV
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