Detection method for oxidative stress functions of different organs of mice

Abstract

The invention belongs to the technical field of new species of vertebrates, and discloses a detection method for oxidative stress functions of different organs of mice. The clean male ICR mice with the weight of 20 g are selected, the mice are randomly grouped, and CdSe / ZnS QDs establishes a poisoned mouse model through tail intravenous injection; low-dosage groups, middle-dosage groups and high-dosage groups of quantum dots QD605 are prepared, and control groups, low-dosage groups, middle-dosage groups and high-dosage groups of quantum dots QD525 are prepared; all the control groups are treated by normal saline; the exposure times of the dosage groups are 11 days, 22 days and 33 days respectively. According to the method, by observing the influence of the quantum dots on genes and proteomes relevant to DNA impairment of the mice and liver and kidney antioxidase, the molecular toxicity effect of the CdSe / ZnS quantum dots on the animal bodies is further disclosed, and basic materials are provided for toxicology invention and bio-safety evaluation of the quantum dots.

Description

technical field [0001] The invention belongs to the technical field of new varieties of vertebrates, and in particular relates to a method for detecting oxidative stress in different organs of mice. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: Quantum dots have great application prospects in the fields of inventions such as medicine and life sciences. However, with the continuous expansion of the application range of quantum dots, their biological safety has gradually attracted widespread attention. Due to the diversity of quantum dot structures and the lack of toxicological data, the toxic mechanism of quantum dots is still not fully understood. Studies have shown that quantum dots can be distributed in various systems in organisms and gather in certain tissues and organs, which are potentially toxic to organisms. The toxicity of quantum dots to cells and organisms is closely related to the type, size, ...

AI Technical Summary

Problems solved by technology

Due to the diversity of quantum dot structures and the lack of toxicological data, the mechanism of quantum dot toxicity to organisms is not yet fully understood.

Therefore, this requires researchers to study the toxicity and mechanism of biological materials from the perspective of the safety of biological materials. For the study of toxicological mechanisms, scientists have done a lot of work on the cytotoxicity of quantum dots in vitro. But there is still a long way to go for the toxicity research of quantum dots in vivo

Images