Human glucagon-like peptide-1 analogue

A technology for glucagon and related peptides, applied in the field of genetic engineering, can solve the problems of many steps, low yield and high cost of chemical synthesis method
CN102796192AInactive Publication Date: 2012-11-28CHINA PHARM UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA PHARM UNIV
Publication Date
2012-11-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the field of gene engineering, and discloses a construction and an expression for two engineering bacteria of two human glucagon-like peptide-1 analogues, and a preparation method for the objective peptides. The objective peptides are formed by inserting objective sequence after double digestions, adding His labels at the front ends of fusion proteins, obtaining objective peptides by cutting the fusion proteins with acid via gaining the fusion proteins, and after an isoelectric precipitation, obtaining the objective peptides again through an affinity chromatography of a Ni agarose gel after an isoelectric precipitation. Freeze-dried objective peptides are dissolved in a PBS buffer solution, oral glucose tolerance test and determination of serum insulin concentration are carried out on normal ICR mice. The two analogues are modified peptides of human glucagon-like peptide-1. Experiments demonstrate that both the two peptides have effects for reducing blood glucose and can be used for treating type 2 diabetes mellitus. Furthermore, the invention also provides the preparation method for the peptides.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering and discloses two human glucagon-related peptide-1 analogs pro-pro-h[Gly8]GLP-1(7-36)D peptide and pro-pro-h[Gly8, Glu22] Construction of GLP-1(7-35)PPSRD peptide engineering bacteria, preparation method of polypeptide and detection of hypoglycemic activity. Using the new fusion expression preparation technology platform for active polypeptides with a length of 30-70aa constructed in our laboratory, we constructed a high-efficiency fusion expression of human glucagon-related peptide-1 analogues in E. coli cells.

[0002] pro-pro-h[Gly8]GLP-1(7-36)D genetically engineered bacteria, the N-terminal alanine (Ala) residue of the human glucagon-related peptide-1 analog is replaced by Gly, and the C-terminal Add D amino acid residues. pro-pro-h [Gly8, Glu22] GLP-1 (7-35) PPSRD genetically engineered bacteria, the alanine (Ala) residue at the N-terminal of the human glucagon-related peptide-1 analog is ...

Claims

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