Preparation method of 3D drug composite stent for polyethylene glycol modified graphene oxide loaded quercetin cross-linked acellular dermal matrix

A decellularized dermis and polyethylene glycol technology, applied in medical science, prostheses, etc., can solve the problems of loss of immune activity, no obvious immunogenicity, etc., to improve water solubility, improve biological functions, and reduce inflammatory reactions Effect

Active Publication Date: 2017-12-29
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

ADM has many advantages: First, ADM removes cellular components, loses the main immune activity of type I and type II cytocompatibil...

Method used

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  • Preparation method of 3D drug composite stent for polyethylene glycol modified graphene oxide loaded quercetin cross-linked acellular dermal matrix
  • Preparation method of 3D drug composite stent for polyethylene glycol modified graphene oxide loaded quercetin cross-linked acellular dermal matrix
  • Preparation method of 3D drug composite stent for polyethylene glycol modified graphene oxide loaded quercetin cross-linked acellular dermal matrix

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Embodiment 1

[0073] A polyethylene glycol-modified graphene oxide-loaded quercetin cross-linked acellular dermal matrix 3D drug composite scaffold for diabetic skin wound repair method, comprising the following steps:

[0074] First, the whole skin of ICR mice was taken to make a circular acellular dermal matrix (ADM) scaffold;

[0075] Secondly, graphene oxide (GO) was catalyzed by EDC, and 6-arm amino polyethylene glycol (PEG) was grafted onto GO to make polyethylene glycol-modified graphene oxide (GO-PEG);

[0076] Then, adsorb quercetin (Que) onto GO-PEG, and the prepared GO-PEG / Que is cross-linked to the surface of ADM scaffold;

[0077] Finally, a 3D drug composite scaffold of polyethylene glycol-modified graphene oxide loaded with quercetin cross-linked acellular dermal matrix (ADM-GO-PEG / Que) was prepared, which can be transplanted into diabetic skin wounds to achieve Delivery of drugs and restoration of wound healing effects.

[0078] Among them, ADM is decellularized dermal mat...

Embodiment 2

[0133] (1) Production of ADM

[0134] 12-week-old male ICR mice were purchased from Guangdong Medical Experimental Animal Center. Neutral protease was purchased from Beijing Aoboxing Biotechnology Co., Ltd. (Beijing, China), and 0.25 g of powder was weighed and dissolved in 100 mL of PBS to prepare a 0.25% neutral protease solution. Both Triton X-100 (Triton X-100) and ethylenediaminetetraacetic acid (EDTA) were purchased from Tianjin Fuchen Chemical Reagent Factory (Tianjin, China), and 28.2mL Triton X-100 was mixed with 72.8mL PBS. Water bath at 37-40°C for 2-3 hours, fully dissolve and mix to make 30% Triton X-100 solution, then take 1mL 30% Triton X-100 and add it to 99mL PBS solution to make 0.3% Triton X-100 solution; Weigh 0.02g EDTA solid powder and dissolve it in 100mL PBS solution to prepare a 0.02% EDTA solution. Absolute ethanol and chloroform were purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Hair removal cream (Veting) was...

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Abstract

The invention discloses a preparation method of a 3D drug composite stent for a polyethylene glycol modified graphene oxide loaded quercetin cross-linked acellular dermal matrix. The preparation method comprises the following steps: firstly, preparing a circular acellular dermal matrix (ADM) stent from ICR rat full skin; secondly, grafting 6-arm amino polyethylene glycol (PEG) to GO to prepare polyethylene glycol modified graphene oxide (GO-PEG) under the condition that graphene oxide (GO) is catalyzed by EDC; thirdly, adsorbing quercetin (Que) to GO-PEG, and cross-linking prepared GO-PEG/Que to the surface of an ADM stent; and finally, preparing the 3D drug composite stent for the polyethylene glycol modified graphene oxide loaded quercetin cross-linked acellular dermal matrix (ADM-GO-PEG/Que). After the composite stent is transplanted to a diabetic skin wound, effects of transferring drug and healing the wound can be achieved.

Description

technical field [0001] The invention relates to the technical field of skin transplantation, in particular to a preparation method of a polyethylene glycol-modified graphene oxide-loaded quercetin-loaded 3D drug composite scaffold cross-linked with acellular dermal matrix. Background technique [0002] Polyethylene glycol-modified graphene oxide (GO-PEG), with high stability and excellent biocompatibility, has attracted more and more attention in the scientific community in recent years as a new type of carrier. . GO-PEG was first reported in 2008. Dai Hongjie’s research group oxidized graphite, then activated the carboxyl group of graphene oxide (GO) under strong alkali conditions, and then prepared polyethylene glycol (PEG) with 6-arm amino group in 1 -Ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC)-catalyzed grafting onto GO yielded a 2D nanocarrier with a size less than 50 nm. GO-PEG has ultra-high drug adsorption capacity, and anticancer drugs such as ca...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/08A61L27/50A61L27/54A61L27/58A61L27/60
CPCA61L27/08A61L27/3633A61L27/3687A61L27/50A61L27/54A61L27/58A61L27/60A61L2300/216A61L2300/412A61L2300/602
Inventor 刘汉平楚婧
Owner SOUTH CHINA NORMAL UNIVERSITY
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