Construction and identification method of super-obese mice

Abstract

The invention discloses a construction and identification method of super-obese mice. The super-obese mice comprise male PPAR alpha- / -mice, female OB / OB mice, a PCR (polymerase chain reaction) amplification instrument, a DNA (deoxyribonucleic acid) amplification product, 2% agarose gel electrophoresis, an identification basis and a leptin gene, wherein a PCR kit is arranged in the PCR amplification instrument; raw materials are arranged in the PCR kit; the raw materials comprise DNA double strands, DNA single strands, primers, TapDNA polymerase, adenine deoxynucleotide, guanine deoxynucleotide, cytosine deoxynucleotide and thymine deoxynucleotide; and the identification basis comprises white adipose tissues, brown adipose tissues, triglyceride, cholesterol, liver, fatty liver, glucose andinsulin. The invention also discloses an identification method of the super-obese mice. The construction and identification method has the advantage that the PPAR alpha delta OB / OB super-obese mice provide a more ideal model for researching obesity and metabolic syndromes thereof and researching the effect of a PPAR alpha signal channel in the obesity occurrence and development process.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for constructing and identifying super obese mice. Background technique [0002] With the improvement of material living standards and changes in life patterns, metabolic syndromes such as obesity, hypertension, hyperlipidemia, diabetes, and atherosclerosis have seriously endangered human health. At present, it is used to study obesity and its metabolic syndrome. The experimental animal models mainly include spontaneous, induced and genetically modified obesity animal models. Commonly used spontaneous obesity mouse models mainly include ob / ob mice, db / db mice, Yellow (Ay / a) mice, etc. , among them, ob / ob mice obesity is related to leptin gene mutation on autosome 6, characterized by gluttony, moderate obesity, hyperglycemia, hyperinsulinemia, hyperlipidemia, glucose and insulin resistance, and low metabolic rate , fatty liver, etc., while db / db mice are spontaneous typ...

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to increase the additional playback delay and the playback fluency is poor. The network status in the playback process cannot be transmitted to the encoding end in time, resulting in the phenomenon of image quality degradation caused by excessive frame skipping and the buffer zone. The problem of playback delay caused by excessive expansion

Images