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Application of chinaroot greenbrier alcohol extract to preparation of weight and fat reducing medicine

A technology of smilax alcohol and medicine, which is applied in the field of smilax alcohol extract, can solve undiscovered problems, achieve the effects of small side effects, inhibit fat deposition, and reduce weight gain

Inactive Publication Date: 2016-03-02
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] So far, no report has been found on the inhibition of fat metabolism in obese mice induced by high-fat diet

Method used

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  • Application of chinaroot greenbrier alcohol extract to preparation of weight and fat reducing medicine
  • Application of chinaroot greenbrier alcohol extract to preparation of weight and fat reducing medicine
  • Application of chinaroot greenbrier alcohol extract to preparation of weight and fat reducing medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of Smilax smilax alcoholic extract and HPLC determination of its polyphenolic components

[0032] Preparation of the alcoholic extract of Smilax smilax:

[0033] S1. Grind sarsaparilla powder, pass through a 60-mesh sieve, add 60% ethanol by volume fraction according to the ratio of solid-liquid ratio of sarsaparilla powder and ethanol to 1g:20ml, and soak for 1~2h;

[0034] S2. Add the solution in S1 to an ultrasonic circulation extractor and sonicate for 30 minutes. After the extraction is completed, centrifuge to take the supernatant and collect the precipitate;

[0035] S3. The precipitate collected in S2 was ultrasonically extracted again by the method of S2, the supernatants of the two times were combined, concentrated by rotary evaporation, and then freeze-dried, and the obtained powder was the alcoholic extract of Smilax smilax. Store in a desiccator for later use.

[0036] The detection method is spectrophotometry, and the content (%) of...

Embodiment 2

[0039] Example 2 Effects of Alcoholic Extract of Smilax smilax on Food Intake, Intra-abdominal Fat and Weight of Each Organ in Mice

[0040] Fifty 4w female ICR mice, weighing about 20g, were randomly divided into 5 groups with 10 mice in each group after being adaptively fed with common feed for one week, namely: normal group, high-fat group, low-dose group (0.25%), Medium dose group (0.5%) and high dose group (1.0%). Free to eat and drink, feed for 8 weeks, weigh once a week, and measure food intake once a day. Feeding conditions: room temperature (24±2°C, light cycle 12L / 12D (light from 08:00 to 20:00).

[0041] After feeding for 8 weeks, the mice were fasted for more than 12 hours. Anesthetized with ether, blood was collected from the heart. After the blood was allowed to stand at room temperature for half an hour, it was centrifuged at 2400r for 15mim, and the serum was aliquoted and stored in an ultra-low temperature refrigerator at -80°C. Tissues such as liver, sple...

Embodiment 3

[0044] Example 3 Effects of the alcoholic extract of Smilax smilax on the activity of enzymes related to lipid metabolism in mouse liver

[0045] Take out the liver tissue stored at -80°C and put it on ice, add pre-cooled homogenization buffer (Tris / HCl3mmol / L, pH7.2; dithiothreitol 1mmol / L; EDTA1mmol) at a weight / volume ratio of 3:20 / L; sucrose 0.25mol / L; ALLN 25μmol / L; leupeptin 100μmol / L; AEBSF 100μmol / L), homogenate on ice. Then, centrifuge at 3000r at 4°C for 20mim, collect the supernatant, aliquot into 5 parts and store at 4°C for testing.

[0046] According to the instruction manual of the enzyme-linked immunosorbent assay kit, the lipid metabolism-related enzymes in the mouse liver, such as acetyl-CoA carboxylase, fatty acid synthase, acyl-CoA oxidase, carnitine ester acyltransferase and AMP-activated protein, were determined. Enzyme activity of kinases. The results are shown in Table 2:

[0047]

[0048] The results showed that the alcohol extract of Smilax smi...

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Abstract

The invention discloses an application of a chinaroot greenbrier alcohol extract to preparation of a weight and fat reducing medicine. The chinaroot greenbrier alcohol extract can suppress cholesterol synthesis, promote fat oxygenolysis, decrease the lipid content of serum and livers, finally suppress fat deposition in an abdominal cavity and reduce increase of the body weight of mice by adjusting expression of lipid metabolism related genes and protein in high fat diet-induced obese mouse livers and fat tissues and adjusting activity of lipolysis related enzyme in the livers. The weight and fat reducing medicine is clear in target, small in side effect and high in clinical effective rate.

Description

technical field [0001] The invention relates to an alcohol extract of smilax as a lipid-lowering and weight-loss drug, in particular to an application of the natural product smilax alcohol extract in preparing a weight-loss and lipid-lowering drug or a drug with a lipid metabolism regulating effect. Background technique [0002] Obesity refers to a certain degree of obvious overweight and excessive fat layer, which is the manifestation of excessive accumulation of neutral fat in the body. In 1998, the World Health Organization defined BMI ≥ 25kg / m 2 and BMI≥30kg / m 2 Defined as overweight and obese, respectively. However, this is based on the European population. According to the epidemiological characteristics of obesity in the Asia-Pacific region, Chinese experts believe that BMI≥25kg / m 2 Overweight, BMI ≥ 27kg / m 2 That is obesity. According to the cause of the disease, obesity is divided into simple obesity and secondary obesity. Simple obesity is the most common typ...

Claims

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Application Information

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IPC IPC(8): A61K36/896A61P3/04A61P3/06
CPCA61K36/896
Inventor 郑国栋杨丽聪林乐珍
Owner JIANGXI AGRICULTURAL UNIVERSITY
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