Application of triptonide

A technology of triptolide ketone and drugs, applied in the field of medicine, to achieve the effects of reducing content, reducing the formation of thoracic aortic atherosclerotic plaque, and reducing body weight

Pending Publication Date: 2019-12-24
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is still a lack of ideal drugs for the treatment of atherosclerosis.

Method used

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  • Application of triptonide
  • Application of triptonide
  • Application of triptonide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Effects of Triptolide Administration on Body Weight and Blood Biochemical Indexes of Mice

[0024] (1) Experimental materials

[0025] (1) Experimental animals:

[0026] Strain: C57-ApoE knockout mouse, male;

[0027] Source: Experimental Animal Center of Soochow University School of Medicine;

[0028] Weight: 18-25g;

[0029] Number of animals in each group: 10.

[0030] (2) Mouse feed formula: maltodextrin (12%), sucrose (18%), cholesterol (2%), imported lard (21%), casein (21%), whole milk powder (5%) , Alfalfa Powder (3%), Corn Starch (8.5%), L-Cystine (0.5%), Vegetable Oil (3%), Premix (6%).

[0031] (3) Test drug: Triptolide was diluted with normal saline to the concentration required for the test.

[0032] (2) Experimental method

[0033] 30 C57-ApoE knockout mice, weighing 18-25 g. They were randomly divided into three groups, 10 rats in each group: high-fat diet group (HFD-TN), high-fat diet+triptolide treatment group (HFD+TN) and normal diet...

Embodiment 2

[0048] Example 2: Triptolide inhibits the formation of atherosclerotic plaque in the thoracic aorta of atherosclerosis model mice

[0049] (1) Experimental materials

[0050] The mouse thoracic aorta was obtained from Example 1; Sudan IV staining solution was purchased from Nanjing Jiancheng Biological Co., Ltd.

[0051] (2) Experimental method

[0052] Before sample collection, the mice were starved overnight, anesthetized with 4% chloral hydrate, dissected, blood was collected from the inferior vena cava, and the heart was perfused. The complete thoracic aorta was removed under the operating microscope and placed in a glass of 4% paraformaldehyde. In a 1.5mL centrifuge tube, fix overnight, stain with Sudan IV staining solution, and peel off the red-stained adipose tissue outside the thoracic aorta with the aid of an operating microscope. Then expand the blood vessel, expose the lumen of the blood vessel, fix it flat in black paraffin, and take pictures with a stereo micros...

Embodiment 3

[0055] Embodiment 3: oil red staining experiment

[0056] (1) Experimental materials

[0057] The mouse tricuspid valve was obtained from Example 1; the oil red staining kit was purchased from Nanjing Jiancheng Biological Co., Ltd.

[0058] (2) Experimental method

[0059] When the mice were dissected, the perfused heart was taken out, fixed overnight with 4% paraformaldehyde, and the root of the heart with the tricuspid valve was cut for embedding with OCT embedding agent, and stored in a -80°C refrigerator for later use. Before staining, the tissues stored in the -80°C refrigerator were taken out and sliced ​​in a cryostat, and the temperature inside the microtome was kept at -20°C. The OCT-embedded tissues were fixed and sliced.

[0060] The thickness of the slice is 12um, and the patch and the adhesive glass slide are placed at room temperature for a while; take out the frozen section, warm up at room temperature, wash off the embedding agent with distilled water; soak ...

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Abstract

The invention relates to the technical field of medicines, and discloses an application of triptonide. The invention finds that the Chinese herbal medicine monomer triptonide can significantly reducethe formation of thoracic aortic atherosclerotic plaques, accumulation of tricuspid valve fat and formation of collagenous fibers of high-fat diet mice, and significantly reduce the weight and fat accumulation of the high-fat diet mice. In addition, the triptonide can also obviously reduce the content of low-density lipoprotein and cholesterol in the blood of the high-fat diet obese mouse. Therefore, the triptonide has the treatment effect on atherosclerosis, so that the triptonide can be applied to the preparation of related medicines, the atherosclerosis is a main reason for coronary heart disease, cerebral infarction and peripheral vascular disease, and the triptonide can also be applied to the preparation of the related medicines for coronary heart disease, cerebral infarction and peripheral vascular disease.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of triptolide. Background technique [0002] Atherosclerosis is the main cause of coronary heart disease, cerebral infarction, and peripheral vascular disease. Atherosclerosis is a common disease that seriously endangers human health. In recent years, the incidence of this disease has increased significantly in our country. According to the results of autopsy, the detection rates of coronary artery and aortic atherosclerotic lesions were 58.36% and 88.31% respectively among people aged 40 to 49, and gradually increased with age. Atherosclerosis generally refers to a group of arteriosclerotic diseases, including: atherosclerosis, Arterial medial calcification and arteriole sclerosis. Risk factors for atherosclerosis Hyperlipidemia It is well known that hyperlipidemia is an important risk factor for atherosclerosis. In fact, hyperlipidemia can also be conside...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/585A61P9/10A61P9/00
CPCA61K31/585A61P9/10A61P9/00
Inventor 周泉生曹志飞
Owner SUZHOU UNIV
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