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Extraction method of soybean oil body having high oxidation resistance

A technology of soybean oil body and extraction method, which is applied in the direction of fat oil/fat production, fat oil/fat refining, fat production, etc. It can solve the problems of easy volatility, emulsifier instability, toxicity, etc., and achieve the effect of easy operation

Active Publication Date: 2015-03-25
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, proteins are mainly used as surfactants at home and abroad. Oil, protein and water are used to produce emulsions through a homogeneous process. They are used as emulsifiers in the food industry. This emulsifier is extremely unstable and the process requires a lot of energy. , and the industry usually uses organic solvents to extract vegetable oil from plant seeds, such as n-hexane, isopropanol, etc. These organic solvents are generally toxic, flammable, explosive, and volatile. In the process of vegetable oil extraction, immersion in organic solvents is easy to cause Denaturation of vegetable protein and easy residue

Method used

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  • Extraction method of soybean oil body having high oxidation resistance
  • Extraction method of soybean oil body having high oxidation resistance
  • Extraction method of soybean oil body having high oxidation resistance

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specific Embodiment approach 1

[0030] Specific Embodiment 1: This embodiment provides a method for extracting soybean oil bodies with high oxidation resistance. It uses soybean seeds as raw materials and extracts them by soaking, grinding, filtering, centrifuging, homogenizing and other processes. The specific steps as follows:

[0031] (1) Soaking: Soak 80g of soybean seeds in 450ml of water for 12 h before use.

[0032] (2) Grinding: Add the seeds with full particles after soaking into a tissue grinder, and grind at 14000 rpm for 2 min. The grinding medium was 1 L of buffer containing 0.6 M sucrose and 10 mM sodium phosphate, pH 7.5.

[0033] (3) Filtration: The homogenate is filtered through three layers of cheesecloth.

[0034] (4) Centrifugal:

[0035] a. Collect the filtrate for sub-packaging and centrifugation, place every 150-200 ml of filtrate at the bottom of a 500-ml centrifuge tube, and use 150-200 ml of flotation medium for each tube (buffer containing 0.4 M sucrose and 10 mM sodium phosphat...

specific Embodiment approach 2

[0044] Specific embodiment two: this embodiment is used as following explanation to the grease body that specific embodiment one prepares:

[0045] 1. Grease body spray drying

[0046] Soybean oil bodies contain a large amount of higher unsaturated fatty acids, especially oleic acid and linoleic acid. However, unsaturated fatty acids in soybean oil body are prone to oxidation, which reduces its nutritional value, and at the same time generates some substances, which have adverse effects on human health. The microencapsulation of soybean oil bodies can effectively avoid the oxidative deterioration of unsaturated fatty acids in soybean oil bodies and prolong the storage period of soybean oil bodies.

[0047] Microencapsulation process: soybean oil body emulsion + wall material → constant temperature stirring → homogenization → emulsion → spray drying → finished microcapsules.

[0048] Weigh a certain amount of soybean protein isolate and maltodextrin (the ratio of soybean ...

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Abstract

The invention discloses an extraction method of a soybean oil body having high oxidation resistance, and belongs to the field of health food or biotechnology. The soybean oil body having high oxidation resistance is extracted from soybean seeds through such processes as soaking, grinding, filtering, centrifuging and homogenizing. According to the extraction method disclosed by the invention, the oil body is extracted by taking water as a medium, so that the method is non-toxic, and simple and convenient to operate, and the soybean oil body is stable, relatively high in purity and conducive to the application in food. The oil body extracted by virtue of the method can be dried or rehydrated and added to any other foods or animal feed products such as peanut butter.

Description

technical field [0001] The invention belongs to the field of health food or biotechnology, and relates to a preparation method of soybean oil bodies with high oxidation resistance and stability. Background technique [0002] Soybean stores lipids as the main storage material in certain subcellular organelle particles, which are dispersed into many small relatively stable subcellular droplets called oil bodies. The particle size of soybean oil bodies is 0.2-0.5 μm, the interior is liquid triacylglycerol, and the exterior is a semi-unit membrane composed of a single layer of phospholipid molecules and their mosaic protein (oleosin). [0003] At present, proteins are mainly used as surfactants at home and abroad. Oil, protein and water are used to produce emulsions through a homogeneous process. They are used as emulsifiers in the food industry. This emulsifier is extremely unstable and the process requires a lot of energy. , and in industry, organic solvents are usually used ...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/04C11B3/16A23L1/035A23L29/10
CPCA23L25/10A23L29/10C11B1/00C11B1/04C11B3/008
Inventor 侯俊财江连洲王胜男梁新婷刘悦王秋岭
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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