An
RNA interference method for controlling
insect pests by inhibiting translation of
messenger RNA (mRNA) of target genes in the
insect's
cholinergic system. The method includes preparing double-stranded
RNA (dsRNA) or single-stranded
antisense oligonucleotides specific to target mRNAs encoding
nicotinic acetylcholine receptor subunits or auxiliary proteins, and administering an effective amount to induce mortality or increase sensitivity to insecticides. The target mRNAs include neuronal α and β subunits of
nicotinic receptors and auxiliary proteins such as RIC-3, Lynx, NACHO, and UNC-50. Administration may be topical, oral, or through transgenic plants expressing the interfering
RNA. The method is particularly effective against agricultural pests like
Acyrthosiphon pisum and can restore sensitivity in insecticide-resistant insects. Compositions including the interfering RNA with
transfection agents or solvents are provided, along with transgenic plants expressing the interfering RNA.