32 results about "Acyrthosiphon pisum" patented technology

The invention discloses a method, a primer and a kit for detecting blue light phototaxis of aphids and relates to the field of agriculture biotechnology. The method for detecting the blue light phototaxis of the aphids disclosed by the invention comprises the following steps: comparing amino acid sequences of CRY1 proteins of target aphids with amino acid sequences of CRY1 proteins of pea aphids; and determining the blue light phototaxis of the target aphids according to sequence comparing results. The detection method is short in time and high in accuracy; the defects such as heavy workload, long time and high possibility of influence of environment factors and the like of the conventional method for detecting phototaxis are avoided; the method is taken as significant technical renovation; the efficiency is greatly improved; the key technical support is provided for early warning and green control of the aphids; the method has an extremely important application value in the field of control of the aphids and can be applied to the fields of control of the aphids, prediction of aphid situation or early warning of the aphids.

The invention provides a purification and expanding propagation method for propylaea japonica. The method comprises the steps that 1, the propylaea japonica is fed in an insect feeding cage, wherein horsebean seedlings are planted and enough pea aphids are fed in the insect feeding cage, a flowerpot of horsebean seedling planting is wrapped with a preservative film, and the number of the stretching blades of the horsebean seedlings is as little as possible so that most of spawn is spawned on the preservative film; 2, the spawned preservative film is collected, and larvae are hatched in a warm and humid condition; 3, the larvae are taken out in time and put into another insect feeding cage to be fed, after the larvae pupate, the spawn collecting operation in the step 2 is stopped; 4, after the spawn in another insect feeding cage in the step 3 is collected through the preservative film, the step 2, the step 3 and the step 4 are repeated until five generations, and the purified spawn is obtained; 5, after the purified spawn is hatched, expanding propagation in the insect feeding cage is conducted. The purification and expanding propagation method is beneficial to purify the generation number of propylaea japonica in the field and reduce newly hatched larvae and imagoes feeding of the spawn indoors, the operation is easy, the cost is low, and purification and expanding propagation for propylaea japonica indoors can be completed effectively.

The invention discloses a low-density breeding method capable of maintaining a wingless state of acyrthosiphon pisum viviparous female. The low-density breeding method includes the steps of planting of broad beans, cultivation of aphids, group experiments, observation and data recording. The low-density breeding method has the advantages of simple operation, stable state, no seasoning restrictionand the like, materials for studying a wing-shaped differentiation mechanism of aphids are provided, and a good foundation is laid for studying the aphid pest control.

The invention provides a biological assaying method for evaluating the effect of transgene cotton on chrysopa pallens. The method comprises the following steps: paving agar on the bottom of a culture dish; placing transgene cotton leaves on the surface of the agar, inoculating newly-hatched helicoverpa armigera larvae; feeding newly-hatched chrysopa pallens larvae for 1 to 3 days with pea aphid, then feeding the chrysopa pallens larvae with the helicoverpa armigera larvae which has been fed by the transgene cotton leaves for 1 to 3 days; observing the growth situation of the chrysopa pallens larvae since the first day when the chrysopa pallens larvae is fed with the helicoverpa armigera larvae until the chrysopa pallens larvae pupate and perform eclosion; setting a reference group; comparing the differences of the experiment group and the reference group on the indexes of pupation rate, eclosion rate, larva weight, imago weight, and total growth time, and analyzing the difference significance through variance analysis so as to evaluate the effect of transgene cotton on chrysopa pallens. The application range of the biological assaying method is wide. The effect of high dose protein in transgene plants on predators can be effectively measured by utilizing the nutrition relationship evaluation of transgene plant-pest-predator.

The invention discloses a method for evaluating the influence of insecticidal protein on adults of propylaea japonica and a feeding device. The invention firstly designs a feeding device for simulating the plant gland, and the suction mode of cotton flower extrafloral nectaries is simulated, and then a cotton juice extract is used as a solvent to dissolve the exogenous insecticidal protein, and fructose is added to serve as a phagostimulant of the propylaea japonica adults; a single-pair of the propylaea japonica adults are put into the feeding device with a feeding solution, and enough pea aphids are supplemented to culture in an illumination cultivation box. According to the indexes of the to-be-detected solution on the death rate of the propylaea japonica adults, the egg laying early stage, the egg laying amount and the weight, whether the insecticidal protein has the influence on the propylaea japonica adults or not is judged. On the base of establishing the risk theory of evaluating that the secondary metabolites and the insecticidal protein in the plant interact to influence the propylaea javanica, the natural environment of the insecticidal protein threat which is faced by the propylaea ladyridis can be reflected more truly, the evaluation method is simple, and the applicability is high.

The invention discloses a method for quickly identifying the resistance of broad beans to pea aphids. The method is to select full-grained broad bean seeds, wash them with clean water, and soak them until they swell completely; place the broad bean seeds evenly in a tray, and alternately light and dark Cultivate and regularly observe the growth of the broad bean seedlings; when the broad bean grows to the 2-3 leaf stage, inoculate 2-3 wingless aphids on the front leaves, and observe their insect sensitivity. The growth and reproduction of aphids are greatly affected by the seasons. Since the environmental conditions in the cultivation room are relatively stable, and the identification system of the present invention is a completely closed system, the repeatability and reliability of the identification results are guaranteed.