9 results about "Acyrthosiphon pisum" patented technology

The invention relates to a novel functional transposase system. More specifically, the invention relates to a DNA transposon comprising a heterologous polynucleotide flanked by transposon flanking regions and to a recombinant transposase of Acyrthosiphon pisum (AP) or Aphis craccivora (AC) comprising an amino acid sequence of at least 90% sequence identity with SEQ ID NO: 4 fused to a heterologous nuclear localization signal, optionally comprising at least one activity improving mutation and to a polynucleotide or an expression vector encoding said DNA transposon or transposase as well as an expression system comprising the transposase / transposon pair. The invention also relates to methods for stably integrating a polynucleotide into cells or stably expressing a protein of interest using the transposase / transposon pair.

The invention discloses a flavonoid compound, a composition and application of the flavonoid compound to prevention and treatment of plant pests, the structure of the flavonoid compound is shown in a formula I, and the flavonoid compound can be used for preventing pests with piercing-sucking mouthparts and pests with rasping-sucking mouthparts, especially pests caused by Amyzus pisum and Odontothrips loti to medicago sativa 'Ladak +', and the flavonoid compound can be used for preventing pests caused by Amyzus pisum and Odontothrips loti to medicago sativa 'Ladak +'. The flavonoid compound disclosed by the invention is derived from a plant secondary metabolite, has the advantages of good environmental compatibility, easiness in degradation, relatively strong target specificity and the like, and accords with the development direction of green pesticides. Therefore, the invention not only provides important material basis and application basis for the development of efficient and safe botanical insecticides, but also has positive significance for promoting the production and ecological sustainable development of the grass industry.

The application discloses a steinernema feltiae biological control agent for preventing and treating various pests and application thereof. The various pests include spodoptera litura, poplar leaf-feeding caterpillar, pea aphid and short horned locust. The application studies the infection activity difference of different strains of steinernema feltiae on four different agricultural pests, and understands the prevention and treatment effect of different strains of steinernema feltiae on different insects. Meanwhile, the application also studies the sensitivity difference of biological control natural enemy insects braconidae and serangium japonicum to steinernema feltiae, studies the infection activity on pests and the infection activity on natural enemy insects, and comprehensively discusses the application prospect of steinernema feltiae, which not only lays a foundation for the joint biological control of natural enemy insects and insect pathogenic nematodes, but also helps to determine the best application scene in agricultural production, and then improves the efficiency and overall effect of biological control, and better prevention and treatment effect can be produced when facing a complex harmful organism prevention and treatment environment.

The invention belongs to the technical field of pesticides, and particularly relates to a preparation method and application of pyridopyrimidinedione containing an isoxazole unit. The compound is shown as a general formula I, wherein X independently represents O or S; r1 independently represents hydrogen, methyl or the like; r2 independently represents an aryl group, a heterocyclic group or the like; the compound has an excellent prevention and treatment effect on pests such as pea aphid, brown planthopper and bemisia tabaci.

The invention belongs to the technical field of agricultural biology, and discloses application of miR-996 in regulation and control of yellow taxis of pea aphids. Researches prove that the api-miR-996 regulates the sensitivity of the pea aphid to yellow stimulation by targeting a target gene ApROR and has a negative regulation effect on the yellow taxis of the pea aphid, the up-regulated expression of the api-miR-996 inhibits the sensitivity of the pea aphid to the yellow stimulation, and the yellow taxis proportion is reduced; by inhibiting the expression of the api-miR-996, the yellowing tendency of the pea aphid is enhanced. The invention provides a new target for regulating and controlling the yellow taxis of the pea aphid, provides a new insight for the molecular mechanism of the yellow taxis behavior of the pea aphid, and provides a theoretical basis for developing a new aphid control pesticide on the basis of the yellow taxis of the pea aphid.

This invention belongs to the field of pesticide and phytochemistry technology, specifically relating to an insecticidal alkaloid compound from *Prunus persica* var. *pinnata*, its preparation method, and its application. The structure of the *Prunus persica* var. *pinnata* alkaloid compound is shown below. This alkaloid compound, isolated from *Prunus persica* var. *pinnata*, is designated as compound Pactermine X. It is a novel alkaloid isolated from *Prunus persica* var. *pinnata*. Its stomach poison and repellency activities against pea aphids were determined. The results showed that compound Pactermine X exhibited strong LC50 activity against pea aphids at 48 h. 50 The concentration was 0.272 mg / mL. At concentrations of 0.5 mg / mL, 0.25 mg / mL, 0.125 mg / mL, and 0.0625 mg / mL, the avoidance rates over 12 hours were 100%, 72.5%, 42.5%, and 30%, respectively.

The invention relates to application of apolipoprotein ApApoD2 in regulation and control of pea aphid lipid synthesis, the research finds that the ApApoD2 is specifically expressed in the epidermis of pea aphid, and after the ApApoD2 gene is effectively silenced by injecting the dsRNA of the ApApoD2, the epidermis lipid coating of the ApApoD2 gene is obviously reduced, the drying tolerance of the ApApoD2 gene is obviously reduced, and the apolipoprotein ApApoD2 can be applied to regulation and control of pea aphid lipid synthesis. By combining transcriptome and non-targeted metabolome analysis, it is found that the ApApoD2 gene regulates horny layer lipid synthesis of the pea aphid and accumulation of various nutrient substances, and the apApoD2 gene is very important for maintaining the lipid homeostasis of the body wall. By inhibiting the expression of the ApApoD2 gene, the lipid synthesis of the pea aphid can be effectively inhibited, so that the drying tolerance of the pea aphid is remarkably reduced, and a new thought and a new method are provided for green prevention and treatment of the pea aphid.

The invention belongs to the technical field of agricultural biology, and particularly relates to an RNA interference sequence of a pisum sativum cathepsin B gene as well as a preparation method and application of the RNA interference sequence. DsRNA of target genes ApCatb-1418 and ApCatb-3483 is synthesized in vitro, and the dsRNA of the pisum sativum is fed by an artificial device, so that the ApCatb-1418 and ApCatb-3483 genes of the pisum sativum can be effectively silenced, the silencing efficiency reaches 64%-74%, and the RNA interference sequence can be applied to the preparation of the pisum sativum cathepsin B gene. The survival rate of the pea aphids can be obviously reduced, the body shape is reduced and the reproductive capacity is reduced; the invention solves the problems of drug resistance, environmental pollution and the like caused by abuse of chemical pesticides in the existing aphid prevention and treatment, and the bottleneck of lack of efficient specific target sequences in RNA interference technology application, and is suitable for developing transgenic aphid-resistant crops or novel nucleic acid biopesticides. The method has important application value in research and development of novel RNAi aphid control insecticides and cultivation of aphid-resistant plants.

The present invention relates to the use of a pea aphid protein having an amino acid sequence as shown in SEQ ID No. 7 and / or SEQ ID No. 8 for antifeeding.