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Rapid identification method for broad bean resistance to Acyrthosiphon pisum

An identification method, pea aphid technology, applied in botany equipment and methods, horticulture, plant cultivation, etc., to achieve the effect of reducing yield and quality loss, ensuring repeatability and reliability

Active Publication Date: 2017-05-10
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Germplasm resources with excellent resistance are the material basis for insect-resistant breeding. However, there are no evaluation criteria and reports on the identification of resistance to aphids in broad beans at home and abroad so far. Therefore, the establishment of germplasm resources for identification of aphids in broad beans method, which is of great significance for the screening of broad bean resistance to pea aphid resources and the research of resistance breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation of embodiment 1 for testing pea aphid

[0031] 1) Mass rearing of pea aphids indoors: the pea aphids are collected from the field of broad beans and subcultured on the broad bean seedlings planted in our laboratory. The temperature is (23±1)°C, the photoperiod is 16L:8D, and the humidity is 75%. .

[0032] 2) Preparation of the test pea aphid: the test nymphs were cultivated in an artificial light incubator by using the improved leaf disc method. Specifically: put fresh broad bean leaves in a petri dish containing an agar layer (1.5%) after soaking them in a pasteurized solution for 10 minutes, select healthy adult aphids and place them on the leaves, cover them, and seal them with a parafilm Prevent pea aphids from crawling out. After 24 hours, the adult aphids in the petri dish were taken out. After the 4th day, 4-day-old nymphs were taken for artificial inoculation experiment. Aphid cultivation conditions are as follows: temperature is (23±1)°C, p...

Embodiment 2

[0033] Example 2 Indoor insect resistance identification

[0034] Each broad bean resource was used as the experimental material, and when the broad bean grew to the 2-3 leaf stage, 2-3 wingless aphids were inoculated on the front leaves. 10 days after pea aphid inoculation, the number of aphids was counted by counting method, and the average number of aphids on the same variety of broad bean plants was obtained, so as to evaluate the resistance according to the following grading standards,

[0035] Grading is based on the ratio of the average amount of aphids per plant per plant, and is divided into 5 levels:

[0036] 1) Level 0: the average ratio of aphids per plant of broad bean is 0-0.25;

[0037] 2) Grade 1: the average ratio of aphids per plant of broad bean is 0.26-0.50;

[0038] 3) Grade 2: the average ratio of aphids per plant of broad bean is 0.51-0.90;

[0039] 4) Grade 3: the average ratio of aphids per plant is 0.91-1.25;

[0040] 5) Grade 4: the average ratio...

Embodiment 3

[0042] Example 3 mung bean resource resistance identification result

[0043] Using the above method, the 4 broad bean resources used in production were identified and evaluated against the pea aphid. The identification results are shown in Table 1.

[0044] Table 1 Identification results of indoor aphid resistance of broad bean

[0045] Variety Average ratio of aphids per plant Degree of resistance Chengdu Dabai 0.4176 R Lincan 5 0.7848 MR Ecan 1 1.0609 S Jing broad beans 130 2.4280 HS

[0046] It can be seen from the above table that there are significant differences in the aphid resistance performance of the various broad bean resources tested, which proves that the identification method provided by the present invention can quickly and accurately distinguish broad bean varieties resistant to aphids and susceptible varieties.

[0047] It can be seen that for the identification of aphid resistance of varieties, the method of ar...

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Abstract

The invention discloses a rapid identification method for broad bean resistance to Acyrthosiphon pisum. The rapid identification method includes: selecting full broad bean seeds, using clean water to clean the broad bean seeds, soaking the broad bean seeds till the broad bean seeds completely expand; evenly arranging the broad bean seeds in a tray, cultivating the broad bean seeds in a lighting-dark alternative condition, and regularly observing the growth condition of broad bean seedlings; and putting 2-3 Acyrthosiphon pisum without wings on a front surfaces of each leaf while each broad bean seedling has 2-3 leaves, and observing the susceptibility of the broad bean seedlings. Growth and reproduction of the Acyrthosiphon pisum is affected by seasons, the environment condition of a cultivation room is stable, and the identification system is a complete closed system, and in this way, the repeatability and the reliability of the identification result can be ensured.

Description

technical field [0001] The invention relates to the technical field of crop resistance breeding, in particular to a rapid identification method for broad bean resistance to pea aphid. Background technique [0002] Broad bean (Vicia faba L.) is an important leguminous crop in the world. Because it has the characteristics of food, vegetables, feed and green manure, and has wide adaptability and high nitrogen fixation capacity, more than 10 countries in the world grow broad beans 70, the planting area is as high as 2.6 million hm 2 . China's broad bean planting area and output both rank first in the world, accounting for 42.76% and 46.94% of the world's total respectively. [0003] The pea aphid (Acyrthosiphon pisum Harris) belongs to the Hemiptera Aphididae and is the main pest of leguminous crops and pastures worldwide. Its host range is very wide. The nymphs and adults of all instars gather in the young parts of plants to feed and cause damage. They suck the juice with pi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00
CPCA01G22/00
Inventor 刘昌燕万正煌李阳焦春海李莉陈宏伟刘良军伍广洪王兴敏
Owner INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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