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Rapid Identification Method of Vicia Bean Resistance to Pea Aphid

An identification method, a pea aphid technology, is applied in the field of rapid identification of broad bean resistance to the pea aphid, achieving the effects of reducing yield and quality loss, ensuring repeatability and reliability

Active Publication Date: 2020-10-27
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Germplasm resources with excellent resistance are the material basis for insect-resistant breeding. However, there are no evaluation criteria and reports on the identification of resistance to aphids in broad beans at home and abroad so far. Therefore, the establishment of germplasm resources for identification of aphids in broad beans method, which is of great significance for the screening of broad bean resistance to pea aphid resources and the research of resistance breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation of embodiment 1 for testing pea aphid

[0031] 1) Mass rearing of pea aphids indoors: the pea aphids are collected from the field of broad beans and subcultured on the broad bean seedlings planted in our laboratory. The temperature is (23±1)°C, the photoperiod is 16L:8D, and the humidity is 75%. .

[0032] 2) Preparation of the test pea aphid: the test nymphs were cultivated in an artificial light incubator by using the improved leaf disc method. Specifically: put fresh broad bean leaves in a petri dish containing an agar layer (1.5%) after soaking them in a pasteurized solution for 10 minutes, select healthy adult aphids and place them on the leaves, cover them, and seal them with a parafilm Prevent pea aphids from crawling out. After 24 hours, the adult aphids in the petri dish were taken out. After the 4th day, 4-day-old nymphs were taken for artificial inoculation experiment. Aphid cultivation conditions are as follows: temperature is (23±1)°C, p...

Embodiment 2

[0033] Example 2 Indoor insect resistance identification

[0034] Each broad bean resource was used as the experimental material, and when the broad bean grew to the 2-3 leaf stage, 2-3 wingless aphids were inoculated on the front leaves. 10 days after pea aphid inoculation, the number of aphids was counted by counting method, and the average number of aphids on the same variety of broad bean plants was obtained, so as to evaluate the resistance according to the following grading standards,

[0035] Grading is based on the ratio of the average amount of aphids per plant per plant, and is divided into 5 levels:

[0036] 1) Level 0: the average ratio of aphids per plant of broad bean is 0-0.25;

[0037] 2) Grade 1: the average ratio of aphids per plant of broad bean is 0.26-0.50;

[0038] 3) Grade 2: the average ratio of aphids per plant of broad bean is 0.51-0.90;

[0039] 4) Grade 3: the average ratio of aphids per plant is 0.91-1.25;

[0040] 5) Grade 4: the average ratio...

Embodiment 3

[0042] Example 3 mung bean resource resistance identification result

[0043] Using the above method, the 4 broad bean resources used in production were identified and evaluated against the pea aphid. The identification results are shown in Table 1.

[0044] Table 1 Identification results of indoor aphid resistance of broad bean

[0045] Variety Average ratio of aphids per plant Degree of resistance Chengdu Dabai 0.4176 R Lincan 5 0.7848 MR Ecan 1 1.0609 S Jing broad beans 130 2.4280 HS

[0046] It can be seen from the above table that there are significant differences in the aphid resistance performance of the various broad bean resources tested, which proves that the identification method provided by the present invention can quickly and accurately distinguish broad bean varieties resistant to aphids and susceptible varieties.

[0047] It can be seen that for the identification of aphid resistance of varieties, the method of ar...

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Abstract

The invention discloses a method for quickly identifying the resistance of broad beans to pea aphids. The method is to select full-grained broad bean seeds, wash them with clean water, and soak them until they swell completely; place the broad bean seeds evenly in a tray, and alternately light and dark Cultivate and regularly observe the growth of the broad bean seedlings; when the broad bean grows to the 2-3 leaf stage, inoculate 2-3 wingless aphids on the front leaves, and observe their insect sensitivity. The growth and reproduction of aphids are greatly affected by the seasons. Since the environmental conditions in the cultivation room are relatively stable, and the identification system of the present invention is a completely closed system, the repeatability and reliability of the identification results are guaranteed.

Description

technical field [0001] The invention relates to the technical field of crop resistance breeding, in particular to a rapid identification method for broad bean resistance to pea aphid. Background technique [0002] Broad bean (Vicia faba L.) is an important leguminous crop in the world. Because it has the characteristics of food, vegetables, feed and green manure, and has wide adaptability and high nitrogen fixation capacity, more than 10 countries in the world grow broad beans 70, the planting area is as high as 2.6 million hm 2 . China's broad bean planting area and output both rank first in the world, accounting for 42.76% and 46.94% of the world's total respectively. [0003] The pea aphid (Acyrthosiphon pisum Harris) belongs to the Hemiptera Aphididae and is the main pest of leguminous crops and pastures worldwide. Its host range is very wide. The nymphs and adults of all instars gather in the young parts of plants to feed and cause damage. They suck the juice with pi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/40A01G7/06
CPCA01G22/00
Inventor 刘昌燕万正煌李阳焦春海李莉陈宏伟刘良军伍广洪王兴敏
Owner INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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