Rapid Identification Method of Vicia Bean Resistance to Pea Aphid

Abstract

The invention discloses a method for quickly identifying the resistance of broad beans to pea aphids. The method is to select full-grained broad bean seeds, wash them with clean water, and soak them until they swell completely; place the broad bean seeds evenly in a tray, and alternately light and dark Cultivate and regularly observe the growth of the broad bean seedlings; when the broad bean grows to the 2-3 leaf stage, inoculate 2-3 wingless aphids on the front leaves, and observe their insect sensitivity. The growth and reproduction of aphids are greatly affected by the seasons. Since the environmental conditions in the cultivation room are relatively stable, and the identification system of the present invention is a completely closed system, the repeatability and reliability of the identification results are guaranteed.

Description

technical field

[0001] The invention relates to the technical field of crop resistance breeding, in particular to a rapid identification method for broad bean resistance to pea aphid. Background technique

[0002] Broad bean (Vicia faba L.) is an important leguminous crop in the world. Because it has the characteristics of food, vegetables, feed and green manure, and has wide adaptability and high nitrogen fixation capacity, more than 10 countries in the world grow broad beans 70, the planting area is as high as 2.6 million hm 2 . China's broad bean planting area and output both rank first in the world, accounting for 42.76% and 46.94% of the world's total respectively.

[0003] The pea aphid (Acyrthosiphon pisum Harris) belongs to the Hemiptera Aphididae and is the main pest of leguminous crops and pastures worldwide. Its host range is very wide. The nymphs and adults of all instars gather in the young parts of plants to feed and cause damage. They suck the juice with pi...