Microfluidic multi-channel online enrichment maldi-tof detection device and method

Abstract

The invention relates to a microfluidic multichannel on-line enriching MALDI-TOF (Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry) detection device and a method. The device comprises an MALDI-TOF target plate, a microfluidic cover plate and a microfluidic device, wherein a plurality of target points are distributed in an array manner on the MALDI-TOF target plate; enriching material layers are arranged on the target points; microfluidic channels are formed in the microfluidic cover plate; the shape and the size of the microfluidic cover are matched with those of the MALDI-TOF target plate; the microfluidic device is used for feeding samples and controlling enriched flow phase. By adopting a microfluidic mode, on-line sample pretreatment can be achieved, a great deal of tedious and complex pretreatment operation such as MALDI-TOF sample enrichment, elution and desalting can be effectively avoided, the time can be effectively shortened, and the detection efficiency and the detection accuracy can be improved.

Description

technical field [0001] The invention belongs to the field of mass spectrometry, and in particular relates to a microfluidic multi-channel online enrichment MALDI-TOF detection device and method. Background technique [0002] Many low-abundance components in complex biological samples carry a large number of disease-related biomarkers, and the separation and detection of these low-abundance samples is also a research hotspot in the fields of biology and chemistry in recent years. Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry MALDI-TOF (Matrix Assisted Laser Desorption Ionization Time of FlightMass Spectrometry) is an efficient and high-resolution technique for component detection in complex biological samples. This technology has been widely used in many fields such as biotechnology, drug research and development, chemical detection, environmental monitoring, etc., and it is also being further expanded to other fields. This method has the advan...

AI Technical Summary

Problems solved by technology

Many cumbersome pretreatment steps are time-consuming and may lead to sample loss, resulting in a decrease in the sensitivity of target detection

It can be seen that the current technology is very inconvenient for screening and testing of large-scale samples.

At the same time, the MALDI-TOF target plates currently used in commercial instruments mainly use reusable target plates, which are expensive and mostly cleaned after work, further increasing the workload, time-consuming and prone to residues

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