Quick in-vivo screening method of antagonistic trichoderma
A screening method, Trichoderma technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of slow re-screening speed of antagonistic Trichoderma, low efficiency of re-screening of antagonistic Trichoderma, and achieve saving materials, solve the heavy workload, and improve the effect of screening efficiency
Active Publication Date: 2018-08-10
SHANGHAI JIAO TONG UNIV
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Abstract
The invention provides a quick in-vivo screening method of antagonistic trichoderma, which comprises the following steps: A, soaking a germinated plant seed in a trichoderma spore suspension for a certain time and then sucking away the trichoderma spore suspension attached to the surface of the plant seed, B, putting a radicle of the plant seed treated in step A downwards in a pathogenic bacteriuminoculation solution, and performing constant temperature illumination culture and seedling emergence, and C, after scanning area S1 of a plant seed leaf after the seedling emergence and comparing the area S1 with area S2 of a seed leaf not inoculated with a pathogenic bacterium and the trichoderma in a control group. A larger ratio leads to a stronger antagonism of the corresponding trichoderma,so that the trichoderma with the excellent antagonism is screened. According to the method, a multi-well plate and a colony area scanner and combined for screening the antagonistic trichoderma, and ascreening result of the method has higher correlation with a potting screening result.
Application Domain
FungiMicroorganism based processes
Technology Topic
SporeScreening method +7
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