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Quick in-vivo screening method of antagonistic trichoderma

A screening method, Trichoderma technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of slow re-screening speed of antagonistic Trichoderma, low efficiency of re-screening of antagonistic Trichoderma, and achieve saving materials, solve the heavy workload, and improve the effect of screening efficiency

Active Publication Date: 2018-08-10
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to provide a fast screening method for live antagonistic Trichoderma to solve the problem of slow re-screening of antagonistic Trichoderma for the low efficiency of traditional antagonistic Trichoderma re-screening

Method used

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  • Quick in-vivo screening method of antagonistic trichoderma
  • Quick in-vivo screening method of antagonistic trichoderma
  • Quick in-vivo screening method of antagonistic trichoderma

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Embodiment 1

[0056] This embodiment relates to a rapid screening method for antagonizing Trichoderma living organisms, comprising the following steps:

[0057]1. Cultivate cucumber anthracnose bacteria (gifted by Li Baoju, a researcher at the Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences) on the PDA plate for 7 days. Take the fungus cake from the edge of the colony, transfer it to PD liquid medium, and cultivate it in a constant temperature shaker at 28°C and 180r / min for 5 days. Transfer the cultured fermentation broth to a centrifuge tube, and centrifuge at 10000rr for 10min in a centrifuge to obtain the germ fermentation supernatant.

[0058] 2. In a 5ml centrifuge tube, mix the supernatant of the bacterial fermentation broth with MS medium at a ratio of 1:4, with a total volume of 4ml, to obtain the bacterial inoculum.

[0059] 3. Put the cucumber seeds (Xiafeng No. 2) in a petri dish covered with moist filter paper, and germinate them in a constant temp...

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Abstract

The invention provides a quick in-vivo screening method of antagonistic trichoderma, which comprises the following steps: A, soaking a germinated plant seed in a trichoderma spore suspension for a certain time and then sucking away the trichoderma spore suspension attached to the surface of the plant seed, B, putting a radicle of the plant seed treated in step A downwards in a pathogenic bacteriuminoculation solution, and performing constant temperature illumination culture and seedling emergence, and C, after scanning area S1 of a plant seed leaf after the seedling emergence and comparing the area S1 with area S2 of a seed leaf not inoculated with a pathogenic bacterium and the trichoderma in a control group. A larger ratio leads to a stronger antagonism of the corresponding trichoderma,so that the trichoderma with the excellent antagonism is screened. According to the method, a multi-well plate and a colony area scanner and combined for screening the antagonistic trichoderma, and ascreening result of the method has higher correlation with a potting screening result.

Description

technical field [0001] The invention belongs to the technical field of biological control, and in particular relates to a rapid screening method for antagonizing Trichoderma living organisms. Background technique [0002] As an important biological control microorganism, Trichoderma has a good control effect on a variety of plant diseases and has been widely used in the field. However, in order to obtain excellent antagonistic Trichoderma, multi-step screening under in vitro and in vivo conditions is required. The traditional screening method mainly includes three steps: plate primary screening, pot plant re-screening and field test. Among them, the re-screening of potted plants is an important part of testing the biocontrol potential of Trichoderma strains, and the workload is relatively large and the test time is relatively long. If there are many candidate Trichoderma strains obtained in the preliminary screening, the re-screening of potted plants will consume more manp...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/885
CPCC12N1/14
Inventor 王强强陈捷窦恺李婷婷
Owner SHANGHAI JIAO TONG UNIV
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