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Application of 4-hydroxylonchocarpin in preparation of medicine for preventing and treating aphids

An aphid and drug technology, applied in the field of pesticides, can solve the problem of no report on aphid killing active substances, and achieve the effects of being environmentally friendly and low in toxicity to humans and animals

Pending Publication Date: 2022-07-15
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many reports on the medicinal activity of 4-hydroxylonchocarpin in recent years, there is no report on the research on pesticide activity, especially as an aphidicidal active substance.

Method used

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  • Application of 4-hydroxylonchocarpin in preparation of medicine for preventing and treating aphids
  • Application of 4-hydroxylonchocarpin in preparation of medicine for preventing and treating aphids
  • Application of 4-hydroxylonchocarpin in preparation of medicine for preventing and treating aphids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Extraction method of 4-hydroxylonchocarpin

[0038]The water chestnut bark of 10kg is pulverized, extracted 3 times with 10L of 95% technical grade absolute ethanol at room temperature; after filtering with Buchner funnel, the filtrate is combined under reduced pressure and concentrated (the temperature of under reduced pressure concentration is 45-60 ℃, pressure: Under the vacuum environment, the time is generally half an hour, using a rotary evaporator) to obtain the water chestnut bark ethanol extract; after the extract is kneaded with water, extracted with 30L petroleum ether, and further concentrated under reduced pressure to obtain 156g of petroleum ether extract; The ether extract was subjected to silica gel column chromatography (the size of the column was 1 m in length, and the silica gel with a diameter of 20 cm was 200-300 mesh), followed by petroleum ether: ethyl acetate = 100:0, 100:1, 50:1, 30 : 1, 20: 1, 10: 1, 5: 1, 3: 1, 1: 1 elution (one drop for 5 sec...

Embodiment 2

[0041] Toxicity assay against aphids

[0042] Use the drip method. The compound 4-Hydroxylonchocarpin prepared in Example 1 was dissolved in acetone to prepare a solution with a concentration of 50 mg / mL, and then diluted with 0.1% Tween-80 water into 1 mg / mL, 0.5 mg / mL, 0.25 mg / mL, 0.125mg / mL, 0.625mg / mL. Pick healthy, wingless aphids of the same size, and drop the medicinal solution on the pronotum of aphids with a 0.3 μL microdropper. After the treatment, the test worms were placed in a 9 cm petri dish with filter paper, placed on the broad bean leaves wrapped with moist cotton in the dish, sealed the petri dish with plastic wrap, and pierced holes for ventilation. Acetone was used as a negative control, and 1 mg / mL matrine was used as a positive control. Each treatment was replicated 3 times with 20 aphids per replicate. Place the treated petri dish in a light incubator with a relative humidity of 60% to 70%, a temperature of 25±1°C, and a light-dark cycle of 16:8. Th...

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Abstract

The invention provides application of 4-hydroxylonchocarpin in preparation of a medicine for preventing and treating aphids, and belongs to the technical field of pesticides. The invention provides an application of 4-hydroxylonchocarpin in the preparation of a medicine for preventing and treating aphids, and relates to the field of medicine for preventing and treating aphids. The 2, 4-hydroxyloncharpin has ideal contact activity on various aphids such as aphis sativa, sitobion avenae and pea aphid, is wide in aphid killing spectrum, is separated from Chinese herbal medicine eleocharis tuberosa peel, has the advantages of being environmentally friendly, high in selectivity, not prone to generating drug resistance of pests and the like, and can be developed as a plant source aphid killing lead compound.

Description

technical field [0001] The invention belongs to the technical field of pesticides, and particularly relates to the application of 4-hydroxylonchocarpin in the preparation of medicines for controlling aphids. Background technique [0002] Aphids belong to the order Hemiptera, which is a general term for insects of the superfamily Aphididae and Aphididae. Aphids can directly damage grain crops such as corn and wheat, as well as economic crops such as vegetables and flowers; and aphids can secrete honeydew to cause the occurrence of various plant diseases such as sooty disease, which has a significant impact on the yield and quality of crops. [0003] At present, the control of aphids at home and abroad mainly relies on chemical agents. The long-term and large-scale use of chemical pesticides not only leads to high resistance of aphids to pesticides such as organophosphorus and pyrethroids, but also brings a series of environmental and ecological problems. Botanical pesticides...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P7/04C07D311/58
CPCA01N43/16C07D311/58Y02A50/30
Inventor 马树杰李晶晶王艺臻高铭张利辉董金皋
Owner HEBEI AGRICULTURAL UNIV.
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