Harmonia axyridia larva indoor industrialized culturing method

A heterochromia, factory-like technology, applied in the field of natural enemy insect breeding, can solve the problems of high cost and workload, cannibalism of larvae, and small number of larvae, so as to increase the workload, save the workload, and facilitate The effect of centralized management and feeding

Pending Publication Date: 2019-01-25
北京阔野田园生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the heterochromia larvae are self-mutilating, and centralized feeding can easily lead to cannibalism of the larvae. Therefore, in order to ensure the survival rate, single-headed or a small amount of centralized feeding is mostly used in feeding.
However, the cost and workload of this feeding method are relatively large, and the number of larvae raised is small, so it is not suitable for application in production. purpose of breeding

Method used

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  • Harmonia axyridia larva indoor industrialized culturing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: the hatching test of heterochromia ladybug egg

[0051] 1. From the 8°C cold storage, select eggs with storage days of 1d, 4d, and 7d for the test. The number of storage days in the cold storage was divided into treatment groups, and each treatment group had 3 repetitions. Egg hatching experiments were carried out at 25°C and 20°C, respectively.

[0052] 2. Calculate the hatching rate of 18 groups of experiments, compare the hatching rate difference under the same storage days and different temperatures, and compare the hatching rate differences at the same temperature and different storage days.

[0053] Table 1 Hatch rate of H. heterochromia eggs in different storage periods

[0054]

[0055] Note: 1d, 4d, and 7d in the treatment group represent the days of storage in cold storage respectively.

[0056] By comparison, it was found that at 25°C, the hatching rate of Heterochromia was high and stable; under the incubation condition of 25°C, the hatch...

Embodiment 2

[0057] The test of embodiment 2 Heterochromia ovum hatching time

[0058] From Example 1, it can be seen that the optimum hatching temperature for the eggs of the heterochromia is 25°C. Eggs stored in cold storage for 1d (group A), 4d (group B) and 7d (group C) were also tested, and the hatching time was counted. Each treatment was replicated three times. Count three times a day at 9:00, 15:00, and 19:00. Record the sum of the three statistics. For example, the first batch of experiments started on September 14, 2018, that is, September 15, 2018 is the first day, recorded as 1d, which is the number of days for eggs to hatch, and so on.

[0059] Table 2 The hatching number and hatching time of different storage time eggs

[0060]

[0061]

[0062] It can be clearly known from the above table that the eggs are concentrated on the third day for hatching, that is, the number of days for hatching is 2 days. That is, eggs stored in cold storage for 1-7 days have no signif...

Embodiment 4

[0064] 1) Select the eggs stored for 1d-4d from the cold storage at 8°C, and place them in a centralized incubation box (breeding conditions are temperature 25±1°C, humidity 70±10%, photoperiod 16L:8D). Place the number of eggs according to the size of the hatching box so that the eggs cover 50% of the bottom area of ​​the hatching box. The hatching box is a transparent plastic box with a cover that can be closed. According to the production target, the number of eggs hatched in the same batch is generally 35% more than the desired number of adults.

[0065] Observe the hatching of the eggs three times a day, and the observation time can be set at 9:00, 14:00, and 17:00. During the last observation, if there are blackened egg masses, they can be taken separately to a unified incubator box, stored overnight in the cold storage, and taken out at 8 o'clock the next day for unified incubation.

[0066] In most cases, the hatching time of the piles is consistent, which is convenie...

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Abstract

The invention discloses a harmonia axyridia larva indoor industrialized culturing method. The method comprises the steps that eggs which are stored in 1-4 days from a cold storage, the eggs are gathered and placed in a hatching box to be hatched, initially hatched larvae are placed in culturing boxes to be cultured; a layer of filter paper is laid at the bottom of each of the culturing boxes, andthe culturing boxes are internally provided with silk-shaped filler to expand the moving range of the larvae; sufficient acyrthosiphon pisum is regularly placed in each culturing box, and horsebean seedlings with cut roots are fixedly placed until the larvae become chrysalides; the feeding of the acyrthosiphon pisum to the harmonia axyridia which becomes a chrysalis is stopped, and the emergence harmonia axyridia is cultured in an insect culturing cage for production. According to the method, industrialized culturing of the harmonia axyridia in the whole developing period is achieved to ensurethe uniformity of the hatching time, the pupation time and the emergence time, convenience is provided for manual concentration management culturing, the killing rate among the harmonia axyridia in the harmonia axyridia larva period is reduced, the duration of egg production of the harmonia axyridia is improved to obtain the adult harmonia axyridia with uniform developing duration and high-yieldeggs, and time and space of industrialized harmonia axyridia propagation are saved.

Description

technical field [0001] The invention relates to the technical field of breeding natural enemy insects, in particular to a method for indoor industrial breeding of ladybug larvae. Background technique [0002] Heterochromia belongs to Coleoptera insects, and it is one of the main natural enemy insects in the field. Both its adults and larvae can prey on aphids, thrips, spider mites, eggs and young larvae of Lepidoptera. Therefore, when the number of heterochromia reaches a certain amount, the number of field aphids and some small pests can be well controlled, and crops can be protected to a certain extent. According to the predation characteristics of Heterochromia, people have applied it to the control of pests in fields and greenhouses to achieve the purpose of biological control. Now, there are more than a dozen biological control companies in the country, most of which sell Heterochromia products. Under the agricultural development trend of "focusing on biological contr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 吴林珂李海燕
Owner 北京阔野田园生物技术有限公司
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